GMPPB Overexpression Lysate

Western Blot: GMPPB Overexpression Lysate (Adult Normal) [NBL1-11145] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GMPPB.

Western Blot: GMPPB Overexpression Lysate (Adult Normal) [NBL1-11145] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for GMPPB.

• Reactivity: Hu
• Applications: WB

GMPPB Overexpression Lysate Summary

• Description: Transient overexpression lysate of GDP-mannose pyrophosphorylase B (GMPPB), transcript variant 2 The lysate was created in HEK293T cells, using Plasmid ID RC200186 and based on accession number NM_021971. The protein contains a C-MYC/DDK Tag.
• Gene: GMPPB

Applications/Dilutions

• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 39.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GMPPB Overexpression Lysate

• EC 2.7.7.13
• GDP-mannose pyrophosphorylase BKIAA1851
• GTP-mannose-1-phosphate guanylyltransferase beta
• mannose-1-phosphate guanyltransferase beta
• mannose-1-phosphate guanylyltransferase

Background

This gene is thought to encode a GDP-mannose pyrophosphorylase. The encoded protein catalyzes the conversion of mannose-1-phosphate and GTP to GDP-mannose, a reaction involved in the production of N-linked oligosaccharides. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]. Transcript Variant: This variant (2) lacks a portion of an exon in the 3' coding region compared to variant 1. The encoded protein (isoform 2) is shorter but has the identical N- and C-termini compared to isoform 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: GMPPB