GASP-1/WFIKKN2 Antibody (288518) [Unconjugated]

GASP-1/WFIKKN2 was detected in immersion fixed paraffin-embedded sections of Human Brain Cortex using Mouse Anti-Human GASP-1/WFIKKN2 Monoclonal Antibody (Catalog # MAB20701) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clone: 288518
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

GASP-1/WFIKKN2 Antibody (288518) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human GASP‑1/WFIKKN2
  Leu35-His576
  Accession # Q8TEU8
• Specificity: Detects human GASP‑1/WFIKKN2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human GASP-2 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: WFIKKN2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GASP-1/WFIKKN2 Antibody (288518) [Unconjugated]

• GASP1
• GASP-1
• Growth and differentiation factor-associated serum protein 1
• hGASP-1
• WAP, follistatin, immunoglobulin, kunitz and NTR domain-containing-relatedprotein
• WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2
• WAP, FS, Ig, two KU and NTR module related protein
• WAP, kazal, immunoglobulin, kunitz and NTR domain-containing protein 2
• WFIKKN2
• WFIKKN-related protein
• WFIKKNRP
• WFIKKNRPmultivalent protease inhibitor protein

Background

Growth and differentiation factor-associated serum protein-1 (GASP-1) was isolated in a screen to identify proteins in mice that copurify with myostatin (GDF-8), a potent negative regulator of skeletal muscle mass (1). The GASP‑1 cDNA encodes a 571 amino acid protein that contains a 29 amino acid signal sequence and is comprised of many conserved domains: WAP, follistatin/Kazal, immunoglobulin, two tandem Kunitz, and netrin (1). Two related human proteins which contain the same domain structure are called WFIKKN (based on the presence and order of these conserved domains) and WFIKKNRP (WFIKKN-related protein) (2). Mouse GASP‑1 is homologous to human WFIKKNRP and mouse GASP‑2 to human WFIKKN. Human GASP‑1 is the same protein as WFIKKNRP.

WAP, follistatin, Kazal and netrin domains are all implicated in protease inhibition, and these proteins may be multivalent protease inhibitors (3). GASP‑1 and -2 show distinct expression patterns both in the developing fetus and the adult. In the developing fetus, GASP‑1 expression is highest in the brain, skeletal muscle, thymus and kidney while GASP‑2 is abundant in the lung, skeletal muscle and liver (4). In the adult, GASP‑1 is primarily expressed in the ovary, testis, and brain while GASP‑2 is in the pancreas, liver, and thymus (4). GASP‑1 inhibits myostatin and the highly related protein, GDF‑11, but not Activin or TGF‑ beta in vitro (1). In addition, GASP‑1 binds directly but independently to both mature myostatin and the myostatin propeptide (1). By amino acid sequence, human GASP‑1 is 90% and 55% identical to mouse GASP‑1 and human GASP‑2, respectively.

1. Hill, J.J. et al. (2003) Mol. Endo. 17:1144.
2. Trexler, M. et al. (2001) Proc. Natl. Acad. Sci. 98:3705.
3. Nagy, A. et al. (2003) Eur. Jour. Biochem. 270:2101.
4. Trexler, M. et al. (2002) Biol. Chem. 383:223.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WFIKKN2
• Uniprot:
  • Q8TEU8()