Gamma Adaptin Antibody - BSA Free

Western Blot: Gamma Adaptin Antibody [NB110-7877] - Analysis shows strong detection of a 91-kDa band corresponding to Human AP1G1 in a HeLa whole cell lysate (lane 1). Peptide competition (using 1 ug/ml of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1 (lane 2). Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP1G1 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. System was used for signal detection (Roche) using a 60-sec exposure time.

Western blot using Affinity Purified anti-AP1G1 antibody shows strong detection of a 91-kDa band corresponding to Human AP1G1. Lane 1: HeLa whole cell lysate

• Reactivity: Hu, Mu, Pm-Or
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: BSA Free

Gamma Adaptin Antibody - BSA Free Summary

• Description: This affinity purified antibody is directed against human Gamma Adaptin. The product was affinity purified from antiserum by immunoaffinity purification
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This affinity purified Gamma Adaptin Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 625-675 of Human Gamma Adaptin. (Uniprot: O43747)
• Localization: Cytoplasmic
• Predicted Species: Mouse (94%), Orangutan (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AP1G1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:2000-1:8000
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:2000
• Application Notes: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in size corresponding to AP1G1 by western blotting in the appropriate cell lysate or extract.
• Control:

  Gamma Adaptin Overexpression Lysate

• Control Peptide:

  Gamma Adaptin Protein (NBP1-96939)

Reactivity Notes

A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee and orangutan based on 100% homology for the immunogen sequence. Cross reactivity with Gamma Adaptin protein from mouse, rat, dog and chicken may occur as sequence homology varies by only one amino acid residue in this sequence as indicated by BLAST analysis. Reactivity with Gamma Adaptin proteins from other sources is not known.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Gamma Adaptin Antibody - BSA Free

• Adapter-related protein complex 1 subunit gamma-1
• Adaptor protein complex AP-1 subunit gamma-1
• adaptor-related protein complex 1, gamma 1 subunit
• ADTGclathrin assembly protein complex 1 gamma large chain
• AP-1 complex subunit gamma-1
• CLAPG1clathrin-associated/assembly/adaptor protein, large, gamma 1
• Clathrin assembly protein complex 1 gamma-1 large chain
• gamma adaptin
• gamma1-adaptin
• golgi adaptor HA1/AP1 adaptin gamma subunit
• Golgi adaptor HA1/AP1 adaptin subunit gamma-1
• MGC18255

Background

AP1G1 is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AP1G1
• Entrez:
  • 164(Human)
  • 11765(Mouse)
  • 171494(Rat)
• Uniprot:
  • O43747(Human)