Gamma Adaptin Antibody (NB110-7877)

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Western Blot: Gamma Adaptin Antibody [NB110-7877] - Analysis shows strong detection of a 91-kDa band corresponding to Human AP1G1 in a HeLa whole cell lysate (lane 1). Peptide competition (using 1 ug/ml of the ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

Gamma Adaptin Antibody Summary

Immunogen
Synthetic peptide: CTAPTSKPSSAGGEL, corresponding to amino acids 646 - 659 of Human AP1G1.
Localization
Cytoplasmic
Specificity
AP1G1
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AP1G1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:2000-1:8000
  • Immunoprecipitation 1:100
Application Notes
This affinity purified antibody has been tested for use in ELISA and by western blot. Expect a band approximately 91 kDa in size corresponding - AP1G1 by western blotting in the appropriate cell lysate or extract.
Positive Control
Gamma Adaptin Lysate (NBL1-07575)
Control Peptide
Gamma Adaptin Protein (NBP1-96939)

Reactivity Notes

Expected to cross-react with Human (100% identity with immunogen), Rat (92% identity with immunogen) and Mouse (92% identity with immunogen) due to sequence homology. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Gamma Adaptin Antibody

  • Adapter-related protein complex 1 subunit gamma-1
  • Adaptor protein complex AP-1 subunit gamma-1
  • adaptor-related protein complex 1, gamma 1 subunit
  • ADTGclathrin assembly protein complex 1 gamma large chain
  • AP-1 complex subunit gamma-1
  • CLAPG1clathrin-associated/assembly/adaptor protein, large, gamma 1
  • Clathrin assembly protein complex 1 gamma-1 large chain
  • gamma adaptin
  • gamma1-adaptin
  • golgi adaptor HA1/AP1 adaptin gamma subunit
  • Golgi adaptor HA1/AP1 adaptin subunit gamma-1
  • MGC18255

Background

AP1G1 is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AP1G1
Entrez