Gamma Adaptin Antibody (100/3)

Western Blot: Gamma Adaptin Antibody (100/3) [NBP2-22155] - Bovine brain extract was separated on SDS-PAGE and probed.

Immunocytochemistry/Immunofluorescence: Gamma Adaptin Antibody (100/3) [NBP2-22155] - 3T3 fibroblasts

Product Discontinued

Gamma Adaptin Antibody (100/3) Summary

• Immunogen: AP-1 adaptor from bovine brain.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: AP1G1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Electron Microscopy 1:10-1:500
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100
• Application Notes: Immunblotting: a minimum working dilution of 1:100 was determined by indirect western blotting using bovine brain extract.

Reactivity Notes

Reacts with Human, Bovine and Primate. This antibody does not react with Rat, Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for Gamma Adaptin Antibody (100/3)

• Adapter-related protein complex 1 subunit gamma-1
• Adaptor protein complex AP-1 subunit gamma-1
• adaptor-related protein complex 1, gamma 1 subunit
• ADTGclathrin assembly protein complex 1 gamma large chain
• AP-1 complex subunit gamma-1
• CLAPG1clathrin-associated/assembly/adaptor protein, large, gamma 1
• Clathrin assembly protein complex 1 gamma-1 large chain
• gamma adaptin
• gamma1-adaptin
• golgi adaptor HA1/AP1 adaptin gamma subunit
• Golgi adaptor HA1/AP1 adaptin subunit gamma-1
• MGC18255

Background

The antibody reacts in immunoblotting with the 104 kDa polypeptide of the Golgi adaptor complex AP-1. It reacts with polypeptides of ~100 kDa in bovine liver, human heart fibroblasts, and Madin-Darby bovine kidney cultured cells (MDBK), but not with any components in the 110-115 kDa range from these sources or from neuroblastoma or astrocytes. This suggests that the 110 and 115 kDa polypeptides may be specific variants occurring only in some cell types of brain. The antibody does not recognize the gamma-subunit in rodents (rat and mouse). In immunofluorescence using MDBK cells, human heart fibroblasts, and African green monkey kidney cells (Vero), the binding of antibody appears to be largely confined to the trans-Golgi network. The antibody has been used for studies on the effects of Brefeldin A, a substance causing rapid redistribution of coat proteins associated with the clathrin-coated vesicles that bud from the trans-Golgi network. The antibody is also useful for the immunoaffinity purification of the Golgi adaptor complex AP-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AP1G1