Fucosyltransferase 11/FUT11 Antibody [Alexa Fluor® 700]

• Applications: WB, IP, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 700

Fucosyltransferase 11/FUT11 Antibody [Alexa Fluor® 700] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Fucosyltransferase 11/FUT11
  Gly25-Leu492
  Accession # AAH36037
• Specificity: Detects human FUT11 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FUT3, rhFUT5, and rhFUT8 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Fucose is frequently found at terminal sites on various glycans and is essential for the generation of many sugar epitopes. Well-known fucose containing glycans include Lewis and ABO blood group antigens. Lewis epitopes are key elements involved in leukocyte homing and extravasation process, thus are essential for lymphocyte maturation and natural defense functions (1). O-fucosylation on Notch receptor is found to be essential for its signaling function (2). So far, 11 carbohydrate specific fucosyltransferases are found in humans (3). FUT1 and FUT2 are alpha 1-2 fucosyltransferases and are responsible for ABO blood group antigen synthesis (4). FUT3, FUT4, FUT5, FUT6, FUT7, and FUT9 are alpha 1-3/4 fucosyltransferases and are responsible for Lewis antigen generation (5). FUT10 and FUT11 are newly cloned alpha 1-3 fucosyltransferases that are distinct from the alpha 1-3/4 fucosyltransferase subfamily and are able to introduce fucose to the innermost core GlcNAc of the N-glycan on conalbumin glycopeptides and biantennary N-glycan acceptors but not onto short lactosaminyl acceptor substrates (6). Predicted as a type II transmembrane protein and a Golgi resident enzyme, the exact function of this enzyme needs to be further characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.