FbxL12 Antibody

Images

 
Immunohistochemistry-Paraffin: FbxL12 Antibody [NB100-1295] - (4ug/ml) staining Human Lung. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC, IHC-P, IP, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

FbxL12 Antibody Summary

Immunogen
Peptide with sequence RACPKESMDWWM corresponding to C-Terminus according to NP_060173.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FBXL12
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 4 - 6 ug/ml
  • Immunoprecipitation 1:10 - 1:500
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Preliminary experiments gave no signal but low background in human brain lysates at up to 1 ug/ml. IHC-P: Human Lung shows staining of ciliated epithelial cells in the bronchioles. IP and ICC uage are reported in scientific literature (PMID: 23707388).
Positive Control
FbxL12 Lysate (NBL1-10613)
Publications
Read Publications using
NB100-1295 in the following applications:

  • 1 publication
  • IP
    1 publication
  • WB
    1 publication

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for FbxL12 Antibody

  • Fbl12
  • F-box and leucine-rich repeat protein 12FLJ20188
  • F-box protein FBL12
  • F-box/LRR-repeat protein 12

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FbxL12 Antibody (NB100-1295)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(1)
IP
(1)
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for FbxL12 Antibody (NB100-1295) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for FbxL12 Antibody (NB100-1295)

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Bioinformatics

Gene Symbol FBXL12
Entrez
Uniprot