FARSB Antibody (2F11) - Azide and BSA Free

Western Blot: FARSB Antibody (2F11) [H00010056-M01] - FARSLB monoclonal antibody (M01), clone 2F11 Analysis of FARSLB expression in HepG2.

Immunohistochemistry-Paraffin: FARSB Antibody (2F11) [H00010056-M01] - Analysis of monoclonal antibody to FARSLB on formalin-fixed paraffin-embedded human cerebellum. Antibody concentration 3 ug/ml.

Immunohistochemistry-Paraffin: FARSB Antibody (2F11) [H00010056-M01] - Analysis of monoclonal antibody to FARSLB on formalin-fixed paraffin-embedded human tonsil tissue. Antibody concentration 3 ug/ml.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 2F11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

FARSB Antibody (2F11) - Azide and BSA Free Summary

• Immunogen: FARSLB (NP_005678, 234 a.a. ~ 341 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PPIINGDHSRITVNTRNIFIECTGTDFTKAKIVLDIIVTMFSEYCENQFTVEAAEVVFPNGKSHTFPELAYRKEMVRADLINKKVGIRETPENLAKLLTRMYLKSEVI
• Specificity: FARSLB - phenylalanine-tRNA synthetase-like, beta subunit
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FARSB
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for FARSB Antibody (2F11) - Azide and BSA Free

• EC 6.1.1
• EC 6.1.1.20
• FARSLBphenylalanine-tRNA ligase beta chain
• FRSBphenylalanine tRNA ligase 1, beta, cytoplasmic
• PheHB
• Phenylalanine--tRNA ligase beta chain
• phenylalanine-tRNA synthetase-like, beta subunit
• phenylalanyl-tRNA synthetase beta chain
• phenylalanyl-tRNA synthetase beta-subunit
• phenylalanyl-tRNA synthetase, beta subunit
• phenylalanyl-tRNA synthetase-like, beta subunit
• PheRS

Background

This gene encodes a highly conserved enzyme that belongs to the aminoacyl-tRNA synthetase class IIc subfamily. This enzyme comprises the regulatory beta subunits that form a tetramer with two catalytic alpha subunits. In the presence of ATP, this tetramer is responsible for attaching L-phenylalanine to the terminal adenosine of the appropriate tRNA. A pseudogene located on chromosome 10 has been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FARSB Antibody (H00010056-M01)(1)

Publication using H00010056-M01

• Xu Z, Lo WS, Beck DB et al. Bi-allelic Mutations in Phe-tRNA Synthetase Associated with a Multi-system Pulmonary Disease Support Non-translational Function. Am J Hum Genet 2018-07-05 [PMID: 29979980]

Bioinformatics

• Gene Symbol: FARSB
• Entrez:
  • 10056(Human)
• OMIM:
  • 609690(Human)
• Uniprot:
  • NP_005678(Human)