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FAAP Overexpression Lysate

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Western Blot: C22orf28 Overexpression Lysate (Adult Normal) [NBL1-08394] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for C22orf28.
Western Blot: C22orf28 Overexpression Lysate (Adult Normal) [NBL1-08394] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for C22orf28.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

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FAAP Overexpression Lysate Summary

Description
Transient overexpression lysate of chromosome 22 open reading frame 28 (C22orf28) The lysate was created in HEK293T cells, using Plasmid ID RC204989 and based on accession number NM_014306. The protein contains a C-MYC/DDK Tag.
Gene
C22ORF28

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for FAAP Overexpression Lysate

  • ankyrin repeat domain 54
  • C22orf28
  • chromosome 22 open reading frame 28
  • DJ149A16.6
  • EC 6.5.1.3
  • HSPC117
  • hypothetical protein LOC51493
  • novel protein HSPC117
  • RP1-149A16.6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line and MBA‑MB‑468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for EphB2 at approximately 139 and 146 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Bioinformatics

Gene Symbol C22ORF28