EMR3 Antibody (908235) [Allophycocyanin]

HEK293 human embryonic kidney cell line transfected with either (A) human EMR3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human EMR3 APC-conjugated Monoclonal Antibody (Catalog # FAB8496A). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human EMR3 APC-conjugated Monoclonal Antibody (Catalog # FAB8496A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 908235
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

EMR3 Antibody (908235) [Allophycocyanin] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: NS0 mouse myeloma cell line transfected with human EMR3
  Met1-Tyr652
  Accession # NP_115960
• Specificity: Stains human EMR3 transfected but not irrelevant transfected cells in flow cytometry.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: EMR3
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EMR3 Antibody (908235) [Allophycocyanin]

• egf-like module containing, mucin-like, hormone receptor-like 3
• EGF-like module receptor 3
• EGF-like module-containing mucin-like hormone receptor-like 3
• egf-like module-containing mucin-like receptor 3
• EMR3

Background

EMR3 is a member of the class B seven-span transmembrane (TM7) receptor family. It is expressed predominantly by cells of the immune system, with highest levels in neutrophils, monocytes and macrophages. It may play a role in myeloid-myeloid interactions during immune and inflammatory responses. EMR3 upregulation in glioblastoma multiforme (GBM) is associated with poor survival and may be a potential therapeutic target.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EMR3
• Uniprot:
  • NP_115960()