EMR3 Antibody (3D7) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: Flow, IP
• Clone: 3D7
• Clonality: Monoclonal
• Host: Armenian Hamster
• Conjugate: CoraFluor 1

EMR3 Antibody (3D7) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
• Immunogen: ARHO-EMR3-CD97 (EGF1) transfectants
• Localization: Cell membrane; Multi-pass membrane protein. EMR3 subunit alpha: Secreted, extracellular space. Note: A soluble form is also detected.
• Specificity: NB100-65935 recognizes human epidermal growth factor (EGF) module-containing mucin-like hormone receptor 3 (EMR3), which is a 56kD member of the EGF-7 transmembrane (TM7) family of adhesion receptors. EMR3 is expressed at the cell surface as a heterodimer. The molecule is predominantly expressed on granulocytes, and at lower levels on mature myeloid cells, monocytes and dendritic cells. EMR3 is absent on lymphocytes, haematopoietic stem cells and myeloid progenitors. Studies suggest that the EMR3 molecule is up-regulated during late stages of neutrophil differentiation and is a marker for terminally differentiated cells. The exact functions of EMR3 and its ligands have not yet been determined.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Armenian Hamster
• Gene: ADGRE3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunoprecipitation
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for EMR3 Antibody (3D7) [CoraFluor™ 1]

• egf-like module containing, mucin-like, hormone receptor-like 3
• EGF-like module receptor 3
• EGF-like module-containing mucin-like hormone receptor-like 3
• egf-like module-containing mucin-like receptor 3
• EMR3

Background

EMR3 is an Orphan-B GPCR with an unknown ligand. Two variants are produced by alternative splicing. One form encodes a 652-amino acid GPCR consisting of two EGF-like domains and a mucin stalk, while the other is a truncated soluble form containing only two EGF-like domains. EMR3 and EMR2 share a high degree of sequence identity in the transmembrane domains. EMR3 has been reported to be expressed in neutrophils, monocytes, and macrophages. ESTs have been isolated from liver/spleen and mixed libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ADGRE3