DYRK4 Overexpression Lysate (Native)

Images

 
Western Blot: DYRK4 Overexpression Lysate (Adult Normal) [NBL1-10078] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DYRK4.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

DYRK4 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC206929 and based on accession number NM_003845. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (DYRK4), mRNA.
Gene
DYRK4

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
59.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for DYRK4

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for DYRK4 Overexpression Lysate (Native)

  • dual specificity tyrosine-phosphorylation-regulated kinase 4
  • dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4
  • EC 2.7.12
  • EC 2.7.12.1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00001859-M01
Species: Hu
Applications: WB, ELISA, IHC, IP
NB110-94452
Species: Hu, Ch, Mu(-), Rt(-)
Applications: WB, ICC/IF
NBP1-33464
Species: Hu, Mu
Applications: WB, ICC/IF
NBP1-92589
Species: Hu, Dr
Applications: WB, ICC/IF, IHC, IHC-P
AF5409
Species: Hu, Mu, Rt
Applications: WB
NBP1-92684
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ICC/IF
NBP1-89396
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF4914
Species: Hu
Applications: WB
NBP1-77127
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-88927
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB100-56633
Species: Hu
Applications: WB
AF2940
Species: Hu
Applications: WB, Flow, CyTOF-ready

Publications for DYRK4 Lysate (NBL1-10078) (0)

There are no publications for DYRK4 Lysate (NBL1-10078).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DYRK4 Lysate (NBL1-10078) (0)

There are no reviews for DYRK4 Lysate (NBL1-10078). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DYRK4 Lysate (NBL1-10078) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional DYRK4 Products

Bioinformatics Tool for DYRK4 Lysate (NBL1-10078)

Discover related pathways, diseases and genes to DYRK4 Lysate (NBL1-10078). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DYRK4 Lysate (NBL1-10078)

Discover more about diseases related to DYRK4 Lysate (NBL1-10078).
 

Pathways for DYRK4 Lysate (NBL1-10078)

View related products by pathway.

PTMs for DYRK4 Lysate (NBL1-10078)

Learn more about PTMs related to DYRK4 Lysate (NBL1-10078).
 

Research Areas for DYRK4 Lysate (NBL1-10078)

Find related products by research area.

Blogs on DYRK4

There are no specific blogs for DYRK4, but you can read our latest blog posts.

Customers Who Bought This Also Bought

DYRK4 Antibody
NBP1-89511

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DYRK4 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol DYRK4