DHTKD1 Antibody

Western Blot: DHTKD1 Antibody [H00055526-B01P] - Analysis of DHTKD1 expression in human liver.

Western Blot: DHTKD1 Antibody [H00055526-B01P] - Analysis of DHTKD1 expression in A-431.

Western Blot: DHTKD1 Antibody [H00055526-B01P] - Analysis of DHTKD1 expression in transfected 293T cell line by DHTKD1 polyclonal antibody. Lane 1: DHTKD1 transfected lysate(101.09 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu, Mu
• Applications: WB, IB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated

DHTKD1 Antibody Summary

• Immunogen: DHTKD1 (NP_061176.3, 1 a.a. - 919 a.a.) full-length human protein. MASATAAAARRGLGRALPLLWRGYQTERGVYGYRPRKPESREPQGALERPPVDHGLARLVTVYCEHGHKAAKINPLFTGQALLENVPEIQALVQTLQGPFHTAGLLNMGKEEASLEEVLVYLNQIYCGQISIETSQLQSQDEKDWFAKRFEELQKETFTTEERKHLSKLMLESQEFDHFLATKFSTVKRYGGEGAESMMGFFHELLKMSAYSGITDVIIGMPHRGRLNLLTGLLQFPPELMFRKMRGLSEFPENFSATGDVLSHLTSSVDLYFGAHHPLHVTMLPNPSHLEAVNPVAVGKTRGRQQSRQDGDYSPDNSAQPGDRVICLQVHGDASFCGQGIVPETFTLSNLPHFRIGGSVHLIVNNQLGYTTPAERGRSSLYCSDIGKLVGCAIIHVNGDSPEEVVRATRLAFEYQRQFRKDVIIDLLCYRQWGHNELDEPFYTNPIMYKIIRARKSIPDTYAEHLIAGGLMTQEEVSEIKSSYYAKLNDHLNNMAHYRPPALNLQAHWQGLAQPEAQITTWSTGVPLDLLRFVGMKSVEVPRELQMHSHLLKTHVQSRMEKMMDGIKLDWATAEALALGSLLAQGFNVRLSGQDVGRGTFSQRHAIVVCQETDDTYIPLNHMDPNQKGFLEVSNSPLSEEAVLGFEYGMSIESPKLLPLWEAQFGDFFNGAQIIFDTFISGGEAKWLLQSGIVILLPHGYDGAGPDHSSCRIERFLQMCDSAEEGVDGDTVNMFVVHPTTPAQYFHLLRRQMVRNFRKPLIVASPKMLLRLPAAVSTLQEMAPGTTFNPVIGDSSVDPKKVKTLVFCSGKHFYSLVKQRESLGAKKHDFAIIRVEELCPFPLDSLQQEMSKYKHVKDHIWSQEEPQNMGPWSFVSPRFEKQLACKLRLVGRPPLPVPAVGIGTVHLHQHEDILAKTFA
• Specificity: Reacts with dehydrogenase E1 and transketolase domain containing 1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: DHTKD1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoblotting
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: This antibody is reactive against tissue and transfected lysate in western blot, and as a detection antibody in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DHTKD1 Antibody

• dehydrogenase E1 and transketolase domain containing 1
• Dehydrogenase E1 and transketolase domain-containing protein 1
• DKFZp762M115
• EC 1.2.4.2
• KIAA1630DKFZP762M115
• MGC3090
• probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial

Background

The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DHTKD1 Antibody (H00055526-B01P)(3)

Publications using H00055526-B01P

• Xu WY, Zhu H, Shen Y et al. DHTKD1 deficiency causes Charcot-Marie-Tooth disease in mice. Mol Cell Biol 2018-04-16 [PMID: 29661920]
• Hagen J, Te Brinke H, Wanders RJ et al. Genetic basis of alpha-aminoadipic and alpha-ketoadipic aciduria. J Inherit Metab Dis 2015-04-10 [PMID: 25860818]
• Xu W, Zhu H, Gu M et al. DHTKD1 is essential for mitochondrial biogenesis and function maintenance. FEBS Lett. 2013-09-27 [PMID: 24076469]

Bioinformatics

• Gene Symbol: DHTKD1
• Entrez:
  • 55526(Human)
• Uniprot:
  • NP_061176.3(Human)