DDX28 Antibody - BSA Free

Western Blot: DDX28 Antibody [NB100-2345] - Detection of Human DDX28 on HeLa whole cell lysate. DDX28 was also immunoprecipitated using rabbit anti-DDX28 antibody NB100-2344.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

DDX28 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 500 and the C-terminus (residue 540) of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 28 using the numbering given in entry NP_060850.1 (GeneID 55794).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX28
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:1000-1:5000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  DDX28 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DDX28 Antibody - BSA Free

• DEAD (Asp-Glu-Ala-Asp) box polypeptide 28
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 28
• EC 3.6.1
• EC 3.6.4.13
• FLJ11282
• M
• MDDX28probable ATP-dependent RNA helicase DDX28
• Mitochondrial DEAD box protein 28
• mitochondrial DEAD-box polypeptide 28

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene is intronless. It encodes an RNA-dependent ATPase. The encoded protein is localized in the mitochondria and the nucleus, and can be transported between the mitochondria and the nucleus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDX28
• Entrez:
  • 55794(Human)
• Uniprot:
  • Q9NUL7(Human)