DAPP1 Antibody

Western Blot: DAPP1 Antibody [NBP2-27542] - HEK293 lysate (10ug protein in RIPA buffer) over expressing Human DAPP1 with DYKDDDDK tag probed with NBP2-27542 (0.1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1:3000) in lane C. Mock-transfected HEK293 probed with MBP2-27542 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DAPP1 Antibody Summary

• Immunogen: Peptide with sequence C-KLSQIRKQLNQGE corresponding to internal region (near C-Terminus) according to NP_055210.2.
• Predicted Species: Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DAPP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: In transfected HEK293 transiently expressing full-length human DAPP1 (myc and DYKDDDDK tagged), a band of approx. 35 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DAPP1 Antibody

• B lymphocyte adapter protein Bam32
• BAM32
• BAM32DKFZp667E0716
• B-cell adapter molecule of 32 kDa
• DAPP1
• dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide
• dual adaptor of phosphotyrosine and 3-phosphoinositides
• hDAPP1

Background

B cell adapter molecule (BAM32) is also designated a dual adapter for phosphotyrosine and 3-phosphotyrosine, and 3-phosphoinositide (DAPP1) or B lymphocyte adapter protein. BAM32 is a B cell-associated adapter that is crucial for B cell antigen receptor signaling regulation. BAM32 interacts with Ptdlns and PLC gamma2 and, upon B cell activation, the protein is phosphorylated on tyrosine residues. It is a mainly cytoplasmic protein that can translocate to the cell membrane after cell stimulation. BAM32, which contains one PH domain and one SH2 domain, is primarily expressed in placenta and lung tissues, but can also be detected in heart, liver, pancreas and brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DAPP1 Antibody (NBP2-27542)(1)

Publication using NBP2-27542

• Niiro H, Allam A, Stoddart A et al. The B lymphocyte adaptor molecule of 32 kilodaltons (Bam32) regulates B cell antigen receptor internalization. J Immunol 2004-11-01 [PMID: 15494510]

Bioinformatics

• Gene Symbol: DAPP1
• Uniprot:
  • NP_055210.2(Human)