DAPP1 Antibody [Alexa Fluor® 594]

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 594

DAPP1 Antibody [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human DAPP1
  Gly2-Ser163
  Accession # Q9UN19
• Specificity: Detects human and mouse DAPP1 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DAPP1 Antibody [Alexa Fluor® 594]

• B lymphocyte adapter protein Bam32
• BAM32
• BAM32DKFZp667E0716
• B-cell adapter molecule of 32 kDa
• DAPP1
• dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide
• dual adaptor of phosphotyrosine and 3-phosphoinositides
• hDAPP1

Background

DAPP1 (Dual Adaptor for Phosphotyrosine and 3-Phosphoinosidides 1; also Bam32 and PHISH) is a 31-32 kDa member of Ig-superfamily of proteins. It shows restricted expression, being found in mast cells, dendritic cells, and germinal center B cells. With respect to B cells, and upon B cell receptor engagement, PI3-kinase is activated, resulting in the generation of membrane-embedded PI(3,4)P2. This product serves as a ligand for cytosolic DAPP1, resulting in its immobilization at the cell membrane. Here, it is phosphorylated on Tyr139, directly regulating HPK1 (hematopoietic progenitor kinase 1) activity, and indirectly regulation HPK1 downstream targets ERK and JNK. Functionally, DAPP1 plays a role in BCR internalization, antibody isotype switching, antigen processing and presentation, and B cell survival. Human DAPP1 is 280 amino acids (aa) in length. It contains one SH2 domain (aa 35‑129), a utilized phosphorylation site at Tyr139 and a C-terminal PH domain (aa 164‑259). When phosphorylated, the observed MW of DAPP1 may be increased by 2-4 kDa in SDS-PAGE. There are four potential alternative splice variants. Two contain a five and 22 aa substitution for aa 259-280, respectively, while a third possesses a 14 aa substitution for aa 1-229, and a fourth shows deletions of aa 35‑75 and aa 180-200 coupled to a three aa substitution for aa 249-280. Over aa 1-163, human DAPP1 shares 91% aa sequence identity with mouse DAPP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.