Recombinant Human Cyclophilin-E His Protein

SDS-Page: Cyclophilin-E Protein [NBC1-18531] - PPIE, 37.5 kDa (337 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

• Reactivity: Hu
• Applications: PAGE, Enzyme Activity
• Concentration: 1 mg/ml

Recombinant Human Cyclophilin-E His Protein Summary

• Description: A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-301 of Human Cyclophilin-E
  
  

  Source: E.coli

  Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMATT KRVLYVGGLA EEVDDKVLHA AFIPFGDITD IQIPLDYETE KHRGFAFVEF ELAEDAAAAI DNMNESELFG RTIRVNLAKP MRIKEGSSRP VWSDDDWLKK FSGKTLEENK EEEGSEPPKA ETQEGEPIAK KARSNPQVYM DIKIGNKPAG RIQMLLRSDV VPMTAENFRC LCTHEKGFGF KGSSFHRIIP QFMCQGGDFT NHNGTGGKSI YGKKFDDENF ILKHTGPGLL SMANSGPNTN GSQFFLTCDK TDWLDGKHVV FGEVTEGLDV LRQIEAQGSK DGKPKQKVII ADCGEYV

• Details of Functionality: Specific activity is > 210 nmol/min/ug, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 1C in Tris-Hcl pH8.0 using chymotrypsin.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PPIE
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 37.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0)
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human Cyclophilin-E His Protein

• Cyclophilin E
• cyclophilin-33
• CYP33
• CyP-33
• EC 5.2.1.8
• MGC111222
• MGC3736
• peptidyl-prolyl cis-trans isomerase E
• peptidylprolyl isomerase E (cyclophilin E)
• PPIase E
• Rotamase E

Background

Cyclophilins possess the activity of peptidyl-prolyl cis-trans isomerase (PPIase) and are involved in cellular protein folding and protein interactions. PPIE contains two RNA binding domains at the N-terminal region and a PPIase domain at the C-terminal region. It was not known what cellular and physiological roles of PPIE. Recombinant human PPIE was expressed in E.coli and purified by conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PPIE