CSRP2BP Overexpression Lysate (Native)

Images

 
Western Blot: CSRP2BP Overexpression Lysate (Adult Normal) [NBL1-09541] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CSRP2BP.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CSRP2BP Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC202620 and based on accession number NM_177926. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens CSRP2 binding protein (CSRP2BP), transcript variant 2, mRNA.
Gene
CSRP2BP

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
74.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CSRP2BP

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CSRP2BP Overexpression Lysate (Native)

  • ADA2A-containing complex subunit 2
  • ATAC component 2 homolog
  • ATAC2CRP2BPKAT14
  • CRP2 binding partner
  • CRP2 binding protein
  • CRP2-binding partner
  • CSRP2 binding protein
  • CSRP2-binding protein
  • cysteine rich protein 2 binding protein
  • cysteine-rich protein 2-binding protein
  • dJ717M23.1
  • MGC15388
  • PRO1194

Background

CSRP2 is a protein containing two LIM domains, which are double zinc finger motifs found in proteins of diverse function. CSRP2 and some related proteins are thought to act as protein adapters, bridging two or more proteins to form a larger protein complex. The protein encoded by this gene binds to one of the LIM domains of CSRP2 and contains an acetyltransferase domain. Although the encoded protein has been detected in the cytoplasm, it is predominantly a nuclear protein. Two transcript variants encoding different isoforms have been found for this gene. Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1, resulting in an isoform (b) that has a shorter N-terminus compared to isoform a.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol CSRP2BP