![Western Blot: CoREST3/RCOR3 Antibody [NB100-60637] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. NB100-60637 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. RCOR3 was also immunoprecipitated by rabbit anti-RCOR3 antibody NB100-60636, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/CoREST3-RCOR3-Antibody-Western-Blot-NB100-60637-img0002.jpg "Western Blot: CoREST3/RCOR3 Antibody [NB100-60637] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. NB100-60637 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. RCOR3 was also immunoprecipitated by rabbit anti-RCOR3 antibody NB100-60636, which recognizes an upstream epitope.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | Novus Biologicals Rabbit CoREST3/RCOR3 Antibody (NB100-60637) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 445 and 495 of human REST corepressor 3 using the numbering given in entry NP_060724.1 (GeneID 55758). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RCOR3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | RCOR3 |
| Entrez | |
| Uniprot |
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![Immunocytochemistry/Immunofluorescence: CoREST3/RCOR3 Antibody [NB100-60637] - FFPE section of NBF-fixed HeLa Cells Antibody: Affinity purified rabbit anti- RCOR3 used at a dilution of 1:80 (2.5 ug/ml). Detection: Green-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight (R) 488 Conjugatedused at a dilution of 1:100.](http://images.novusbio.com/fullsize/CoREST3-RCOR3-Antibody-Immunocytochemistry-NB100-60637-img0004.jpg "Immunocytochemistry/Immunofluorescence: CoREST3/RCOR3 Antibody [NB100-60637] - FFPE section of NBF-fixed HeLa Cells Antibody: Affinity purified rabbit anti- RCOR3 used at a dilution of 1:80 (2.5 ug/ml). Detection: Green-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight (R) 488 Conjugatedused at a dilution of 1:100.")
![Immunohistochemistry: CoREST3/RCOR3 Antibody [NB100-60637] - Sample: FFPE sections of human testicular seminoma (left) and breast carcinoma (right). Antibody: Affinity purified rabbit anti- RCOR3 used at a dilution of 1:200 (1ug/ml) and 1:80 (2.5 ug/ml). Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG-heavy](http://images.novusbio.com/fullsize/CoREST3-RCOR3-Antibody-Immunohistochemistry-NB100-60637-img0003.jpg "Immunohistochemistry: CoREST3/RCOR3 Antibody [NB100-60637] - Sample: FFPE sections of human testicular seminoma (left) and breast carcinoma (right). Antibody: Affinity purified rabbit anti- RCOR3 used at a dilution of 1:200 (1ug/ml) and 1:80 (2.5 ug/ml). Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG-heavy")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")