COPB2 Antibody

Product Discontinued

COPB2 Antibody Summary

• Immunogen: Synthetic peptide corresponding to residues E(496) A G E L K P E E E I T V G P V Q K(513) of rat beta-COP.
• Specificity: Beta COP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COPB2
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:2000
  • Western Blot 1:1000
• Application Notes: WB: Detects an approx. 110kDa protein which represents beta-COP from rat brain homogenate. Indirect IF: Staining of beta-COP in NIH-3T3 cells with NB120-2899 results in beta-COP localization to the Golgi complex and to distinct vesicular structures scattered throughout the cytoplasm. Golgi localization of beta-COP is sensitive to treatment with brefeldin A or ATP depletion. Use in FLOW cytometry reported in scientific literature ( PMID 27510922).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, containing 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 4.4 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for COPB2 Antibody

• beta prime subunit
• Beta'-coat protein
• Beta'-COP
• betaprime-COP
• coatomer protein complex, subunit beta 2 (beta prime)
• coatomer subunit beta'
• p102

Background

Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COP I and COP II) and although they have mechanistic parallels, they are molecularly distinct. The COP I coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COP I) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COP I, and ARF. COP I has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COP II facilitates anterograde transport between these subcellular organelles. COP II has been shown to be independently and selectively recruited to the ER relative to COP I subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for COPB2 Antibody (NB120-2899)(3)

Publications using NB120-2899

• Karanasios E, Walker SA, Okkenhaug H et al. Autophagy initiation by ULK complex assembly on ER tubulovesicular regions marked by ATG9 vesicles. Nat Commun. 2016-08-11 [PMID: 27510922] (FLOW, Mouse)
• Suarez-Causado A, Caballero-Diaz D, Bertran E et al. HGF/c-Met signaling promotes liver progenitor cell migration and invasion by an epithelial-mesenchymal transition-independent, phosphatidyl inositol-3 kinase-dependent pathway in an in vitro model. Biochim. Biophys. Acta. 2015-05-20 [PMID: 26001768] (ICC/IF, Mouse)
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (Human)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

Bioinformatics

• Gene Symbol: COPB2
• Entrez:
  • 9276(Human)
  • 60384(Rat)
• Uniprot:
  • 70349(Mouse)
  • O35142(Rat)