Cofilin [p Tyr140, p Ser156] Antibody

Product Discontinued

Cofilin [p Tyr140, p Ser156] Antibody Summary

• Immunogen: A synthetic peptide from the phospho Y140/S156 of human Cofilin-1 (Cofilin, non-muscle isoform) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat, mouse and monkey.
• Modification: p Tyr140, p Ser156
• Localization: subcellular location: nucleus matrix. cytoplasm, cytoskeleton. note: almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide. tissue specificity: widely distributed in various tissues.
• Specificity: Cofilin-1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CFL1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 10-50 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 10-50 ug/ml
• Control:

  Cofilin Overexpression Lysate

Reactivity Notes

Mouse, Rat and Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: IgG purified
• Reconstitution Instructions: Reconstitute in 500 ul of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability.

Alternate Names for Cofilin [p Tyr140, p Ser156] Antibody

• 18 kDa phosphoprotein
• CFL
• CFL1
• cofilin 1 (non-muscle)
• cofilin
• Cofilin, non-muscle isoform
• cofilin-1
• HEL-S-15
• p18

Background

Cofilin is a small phosphoinositide sensitive, actin binding protein capable of depolymerizing actin filaments in vitro. Under certain conditions, it fragments the filaments and accelerates actin subunit dissociation from their ‘pointed’ (minus) ends. Cofilin binds stoichiometrically to monomeric G-actin and to actin protomers in filaments in an apparent pH-dependent, Ca2+ independent manner. Cofilin intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist. Cofilin is ubiquitous in tissues of eukaryotes and is especially abundant in neuronal tissues. It is essential for viability and is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, cytokinesis, and pathogen intracellular motility.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CFL1
• Entrez:
  • 1072(Human)
  • 12631(Mouse)
  • 29271(Rat)
• Uniprot:
  • P23528(Human)