CLIC1 Antibody (2D4)

Western Blot: CLIC1 Antibody (2D4) [H00001192-M01] - CLIC1 monoclonal antibody (M01), clone 2D4 Analysis of CLIC1 expression in HL-60.

Immunocytochemistry/Immunofluorescence: CLIC1 Antibody (2D4) [H00001192-M01] - Analysis of monoclonal antibody to CLIC1 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: CLIC1 Antibody (2D4) [H00001192-M01] - Analysis of monoclonal antibody to CLIC1 on formalin-fixed paraffin-embedded human colon tissue. Antibody concentration 3 ug/ml.

ELISA: CLIC1 Antibody (2D4) [H00001192-M01] - Detection limit for recombinant GST tagged CLIC1 is 1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P
• Clone: 2D4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CLIC1 Antibody (2D4) Summary

• Immunogen: CLIC1 (AAH64527.1, 1 a.a. ~ 241 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAEEQPQVELFVKAGSDGAKIGNCPFSQRLFMVLWLKGVTFNVTTVDTKRRTETVQKLCPGGQLPFLLYGTEVHTDTNKIEEFLEAVLCPPRYPKLAALNPESNTAGLDIFAKFSAYIKNSSPALNDNLEKGLLKALKVLDNYLTSPLPEEVDETSAEDEGVSQRKFLDGNELTLADCNLLPKLHIVQVVCKKYRGFTIPEAFRGVHRYLSNAYAREEFASTCPDDEEIELAYEQVAKALK
• Specificity: CLIC1 - chloride intracellular channel 1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLIC1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CLIC1 Antibody (2D4)

• Chloride channel ABP
• chloride intracellular channel 1
• chloride intracellular channel protein 1
• G6
• hRNCC
• NCC27p64CLCP
• Nuclear chloride ion channel 27
• nuclear chloride ion channel protein
• Regulatory nuclear chloride ion channel protein
• RNCC protein

Background

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family; the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CLIC1 Antibody (H00001192-M01)(2)

Publications using H00001192-M01

• Poschmann G, Grzendowski M, Stefanski A et al. Redox proteomics reveal stress responsive proteins linking peroxiredoxin-1 status in glioma to chemosensitivity and oxidative stress. Biochim Biophys Acta. 2014 Dec 04 [PMID: 25484280]
• Megger DA, Bracht T, Kohl M et al. Proteomic differences between hepatocellular carcinoma and nontumorous liver tissue investigated by a combined gel-based and label-free quantitative proteomics study. Mol Cell Proteomics. 2013 Jul [PMID: 23462207] (Human)

Bioinformatics

• Gene Symbol: CLIC1
• Entrez:
  • 1192(Human)
• Uniprot:
  • AAH64527(Human)