CEP350 Antibody

Immunoprecipitation: CEP350 Antibody [NB100-59810] - Detection of Human CAP350 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CAP350 antibody NB100-59810 used for IP at 3 mcg/mg lysate. CAP350 was also immunoprecipitated by rabbit anti-CAP350 antibody NB100-59811, which recognizes a downstream epitope.

Product Discontinued

CEP350 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 450 and 500 of human centrosome-associated protein 350 using the numbering given in entry NP_055625.3 (GeneID 9857).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CEP350
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation 2 to 5 ug/mg
  • Western Blot
• Application Notes: Use in WB and ICC/IF reported in scientific literature (PMID: 25764135).

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 25764135).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CEP350 Antibody

• CAP350GM133
• centrosomal protein 350kDa
• centrosome-associated protein 350
• Centrosome-associated protein of 350 kDa
• Cep350
• FLJ38282
• FLJ44058
• KIAA0480centrosome associated protein 350

Background

CAP350 (Centrosome-Associated Protein 350) has been shown to localize to the centrosome as well as to dots in the pericentrosomal area. CAP350 functions to stabilize and anchor microtubules to the centrosome and participates in the maintenance of a pericentrosomal Golgi ribbon.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CEP350 Antibody (NB100-59810)(1)

Publication using NB100-59810

• Gavilan MP, Arjona M, Zurbano A et al. Alpha-catenin-Dependent Recruitment of the Centrosomal Protein CAP350 to Adherens Junctions Allows Epithelial Cells to Acquire a Columnar Shape. PLoS Biol. 2015-03-01 [PMID: 25764135] (ICC/IF, WB, Canine)

Bioinformatics

• Gene Symbol: CEP350
• Entrez:
  • 9857(Human)
• Uniprot:
  • NP_055625.3(Human)