CELSR2 Antibody [Alexa Fluor® 405]

• Reactivity: Hu, Mu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 405

CELSR2 Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human CELSR2
  Cys51-Phe231
  Accession # Q9HCU4
• Specificity: Detects human CELSR2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) CELSR1 and rhCELSR3 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CELSR2 Antibody [Alexa Fluor® 405]

• cadherin EGF LAG seven-pass G-type receptor 2
• Cadherin family member 10
• cadherin, EGF LAG seven-pass G-type receptor 2 (flamingo homolog, Drosophila)
• CDHF10
• CDHF10FLJ34118
• CELSR2
• EGFL2
• EGFL2FLJ45143
• EGF-like protein 2
• EGF-like-domain, multiple 2
• epidermal growth factor-like 2
• Epidermal growth factor-like protein 2
• Flamingo homolog 3
• Flamingo1
• FLJ45845
• KIAA0279FLJ42737
• MEGF3
• MEGF3cadherin, EGF LAG seven-pass G-type receptor 2, flamingo (Drosophila) homolog
• Multiple EGF-like domains protein 3
• multiple epidermal growth factor-like domains 3
• Multiple epidermal growth factor-like domains protein 3

Background

CELSR2 (Cadherin EGF LAG seven-pass G-type receptor 2; also cadherin family member 10/CDHF10, Flamingo1 and EGFL2) is a 300-330 kDa member of the LN‑7TM subfamily, GPCR 2 family of proteins. It is expressed on neurons, breast epithelium, Sertoli cells and germ cells, and through homophilic interactions, serves as either an adhesion or guidance molecule. Mature human CELSR2 is 2892 amino acids in length (aa 32-2923). It is a highly complex 7-transmembrane protein that contains a 2349 aa extended N-terminal extracellular region (aa 32-2380) plus a 310 aa C-terminal cytoplasmic domain. The N-terminal region contains nine consecutive cadherin domains (aa 182-1146) followed by a mixture of seven EGF-like and three laminin-like domains. There is a proteolytic cleavage site between Met2356-Thr2357 that generates a 250 kDa soluble fragment and a (mature) 60-65 kDa transmembrane segment that may reside on the cell membrane. Over aa 51‑231, human CELSR2 shares 93% aa identity with mouse CELSR2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.