CELSR2 Antibody

Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human CELSR2. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human CELSR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6739) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CELSR2 at approximately 240 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

SH‑SY5Y human neuroblastoma cell line was stained with Goat Anti-Human CELSR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6739, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Human CELSR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6739, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

CELSR2 was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human CELSR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6739) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to ductal epithelium. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Product Discontinued

CELSR2 Antibody Summary

• Immunogen: E. coli-derived recombinant human CELSR2
  Cys51-Phe231
  Accession # Q9HCU4
• Specificity: Detects human CELSR2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) CELSR1 and rhCELSR3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CELSR2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CELSR2 Antibody

• cadherin EGF LAG seven-pass G-type receptor 2
• Cadherin family member 10
• cadherin, EGF LAG seven-pass G-type receptor 2 (flamingo homolog, Drosophila)
• CDHF10
• CDHF10FLJ34118
• CELSR2
• EGFL2
• EGFL2FLJ45143
• EGF-like protein 2
• EGF-like-domain, multiple 2
• epidermal growth factor-like 2
• Epidermal growth factor-like protein 2
• Flamingo homolog 3
• Flamingo1
• FLJ45845
• KIAA0279FLJ42737
• MEGF3
• MEGF3cadherin, EGF LAG seven-pass G-type receptor 2, flamingo (Drosophila) homolog
• Multiple EGF-like domains protein 3
• multiple epidermal growth factor-like domains 3
• Multiple epidermal growth factor-like domains protein 3

Background

CELSR2 (Cadherin EGF LAG seven-pass G-type receptor 2; also cadherin family member 10/CDHF10, Flamingo1 and EGFL2) is a 300-330 kDa member of the LN-7TM subfamily, GPCR 2 family of proteins. It is expressed on neurons, breast epithelium, Sertoli cells and germ cells, and through homophilic interactions, serves as either an adhesion or guidance molecule. Mature human CELSR2 is 2892 amino acids in length (aa 32-2923). It is a highly complex 7-transmembrane protein that contains a 2349 aa extended N-terminal extracellular region (aa 32-2380) plus a 310 aa C-terminal cytoplasmic domain. The N-terminal region contains nine consecutive cadherin domains (aa 182-1146) followed by a mixture of seven EGF-like and three laminin-like domains. There is a proteolytic cleavage site between Met2356-Thr2357 that generates a 250 kDa soluble fragment and a (mature) 60-65 kDa transmembrane segment that may reside on the cell membrane. Over aa 51-231, human CELSR2 shares 93% aa identity with mouse CELSR2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CELSR2 Antibody (AF6739)(2)

Publications using AF6739

• Lena P. Basta, Parijat Sil, Rebecca A. Jones, Katherine A. Little, Gabriela Hayward-Lara, Danelle Devenport Celsr1 and Celsr2 exhibit distinct adhesive interactions and contributions to planar cell polarity Frontiers in Cell and Developmental Biology 1/12/2023 [PMID: 36712970]
• Xin Wang SNX27 Deletion Causes Hydrocephalus by Impairing Ependymal Cell Differentiation and Ciliogenesis J. Neurosci, 2016-12-14;36(50):12586-12597. 2016-12-14 [PMID: 27974614] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: CELSR2
• Uniprot:
  • Q9HCU4(Human)