CEACAM2 Antibody (935610) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with either (A) mouse CEACAM-2 or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse CEACAM-2 Monoclonal Antibody (Catalog # MAB9214) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB005). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 935610
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

CEACAM2 Antibody (935610) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CEACAM-2
  Gln35-Asp422
  Accession # Q925P2
• Specificity: Detects mouse CEACAM-2 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CEACAM2 Antibody (935610) [Unconjugated]

• AI267129
• Bgp;Bgp2;Carcinoembryonic antigen-related cell adhesion molecule 2;Ceacam2-L
• Bgp2
• CEACAM2
• CEACAM-2
• Ceacam2-L

Background

CEACAM2 is a member of the family of carcinoembryonic antigen-related cell adhesion molecules. CEACAM2 controls energy balance and peripheral insulin action. It is involved in the regulation of feeding behavior particularly in the ventromedial nucleus of hypothalamus (VMH) regulation of food intake. Alternatively spliced transcripts encoding different proteins have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q925P2()