Caspase Antibody Pack

Product Discontinued

Caspase Antibody Pack Summary

• Immunogen: Caspase-1, 0.025mg, 371 RKVRFSFEQPDGRAQMPTTE 390, WB, IHC (paraffin), H, M, Western blot: pro and active forms seen
  Caspase-2, 0.05ml, 400 REGYAPGTEFHRC 412, WB, H
  Caspase-3 (Pro and Active/Cleaved), 0.025mg, Localized to the large domain subunit, WB, IHC (paraffin), IHC (frozen), H, M, R, Western blot: pro and active forms seen
  Caspase-3 (Pro and Active/Cleaved), 0.025mg, Recombinant Caspase-3 protein, WB, H, Western blot: pro and active forms seen
  Caspase-6 (Pro and Active/Cleaved), 0.025ml, Recombinant catalytically active protein, WB, IHC (paraffin), IHC (frozen), H, M, R, D, G, Active form preferentially seen
  Caspase-7 (Pro and Active/Cleaved), 0.025mg, Recombinant Caspase-7 protein, WB, H, M, R, Western blot: pro and active forms seen
  Caspase-8 (Pro and Active/Cleaved), 0.025mg, 370 PVETDSEEQP 379, WB, H, Ch, Rh, Western blot: pro and active forms seen
  Caspase-9, 0.025ml, Recombinant catalytically active protein, WB, IHC, IP, Immuno-EM, H, M, R, Western blot: pro and active forms seen
  Caspase-10, 0.025mg, 1 MKSQGQHWYSSSDKN 15, WB, H, M, R, C,
  Caspase-14, 0.025ml, 2 ESEMSDPQPLQEERYDM 18, WB, IHC, IP, H, M, R, D, G, Western blot: pro and active forms seen
  H: Human, M: Mouse, R: Rat, C: Chicken, D: Dog, G: Gerbil, Ch: Chimpanzee, Rh: Rhesus monkey

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Simple Western
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background

Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.