Carbohydrate Sulfotransferase 5/CHST5 Antibody [Alexa Fluor® 488]

• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

Carbohydrate Sulfotransferase 5/CHST5 Antibody [Alexa Fluor® 488] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Carbohydrate Sulfotransferase 5/CHST5
  Ser28-Arg390
  Accession # Q9GZS9
• Specificity: Detects human Carbohydrate Sulfotransferase 5/CHST5 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) CHST1, rhCHST2, rmCHST5, and rmCHST7 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

CHST5 (Carbohydrate sulfotransferase 5; also GlcNAc6ST-3, I-GlcNAc6ST and GST4 alpha ) is likely a 53‑56 kDa member of the sulfotransfease family of enzymes. It is a Golgi-embedded enzyme that is found in T cells, B cells and intestinal epithelium. It appears to act only on short carbohydrates and transfers sulfate to position # 6 of mucin-associated GlcNAc. Human CHST5 is a 390 amino acid (aa) type II transmembrane protein. It contains a short cytoplasmic segment (aa 1‑9) and a long luminal domain (aa 28‑390) that contains an enzymatic region (aa 180‑358). There is one alternate start site that is 22 aa upstream of the standard site. Humans have a GST4-alpha and -beta gene, the result of gene duplication. Over aa 28‑390, these two gene products share 87% aa identity. Mouse has only one GST4 gene, and it is functionally related to human GST4-beta . Over aa 28‑390, human GST4‑ alpha /CHST5 is 78% aa identical to mouse GST4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.