Carbohydrate Sulfotransferase 1/CHST1/KS6ST Antibody (543128) [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: IP, B/N
• Clone: 543128
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

Carbohydrate Sulfotransferase 1/CHST1/KS6ST Antibody (543128) [Alexa Fluor® 647] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Carbohydrate Sulfotransferase 1/CHST1
  Arg24-Ser411
  Accession # O43916
• Specificity: Detects human Carbohydrate Sulfotransferase 1/CHST1 is direct ELISAs. In direct ELISAs, approximately 25-100% cross-reactivity with recombinant mouse CHST1 and no cross-reactivity with recombinant human CHST2, 3, 4, 5, 6, 7, 10, and 14 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Neutralization

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbohydrate Sulfotransferase 1/CHST1/KS6ST Antibody (543128) [Alexa Fluor® 647]

• 6-O-sulfotransferase 1
• C6ST
• carbohydrate (chondroitin 6/keratan) sulfotransferase 1
• carbohydrate (keratan sulfate Gal-6) sulfotransferase 1
• Carbohydrate Sulfotransferase 1
• CHST1
• EC 2.8.2
• Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1
• GST-1
• Keratan sulfate Gal-6 sulfotransferase
• KS6ST
• KSGal6ST
• KSGal6STEC 2.8.2.21
• KSST

Background

The CHST family is comprised of 14 enzymes in humans. All members of this family are Golgi-localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (1). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (2). CHST1, also known as keratan sulfate Gal-6 sulfotransferase, transfers sulfate to position 6 of galactose residues on keratan sulfate (3). It also has sulfotransferase activity on sialyl N-acetyllactosamine structures and participates in biosynthesis of selectin ligands that play a central role in lymphocyte homing at sites of inflammation (4). Human CHST1 shares 94% amino acid sequence identity with mouse CHST1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.