BTBD7 Antibody

Images

 
Western Blot: BTBD7 Antibody [NBP1-49652] - Lane A: Immune Serum, Lane B: Preimmune. Apparent MW is 138 kDa

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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Catalog# & Conjugate Size Price

BTBD7 Antibody Summary

Immunogen
Synthetic peptide corresponding to unique epitope on Btbd7 protein between aa 1095-1138. The peptide sequence selected was post synthetically modified to achieve desired antigenicity before covalently coupling to a carrier protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BTBD7
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:200
Application Notes
Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 23325674)
Control Peptide
BTBD7 Protein (NBP1-49654)
Publications
Read Publications using
NBP1-49652 in the following applications:

  • 2 publications
  • WB
    2 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 11231300)

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris/Glycine buffer pH 7.5-7.9, stabilizing protein, and glycerol
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BTBD7 Antibody

  • BTB (POZ) domain containing 7
  • DKFZp686N0544
  • FLJ10648
  • FUP1BTB/POZ domain-containing protein 7
  • KIAA1525
  • MGC48310

Background

Cancer cells especially human hepatocellular carcinoma (HCC) differentially express many genes as determined by cDNA microarrays. One of these genes is Btbd7 whose function was not known. When transfected in NIH3T3 cells this gene exhibited enhanced cell proliferation activity by MTT assay. The enhanced tumorigencity was blocked by anti-sense RNA experiments (1). Recently it has been shown that Btbd7 regulate epithelial cell dynamics and branching morphogenesis. The organ development during embryogenesis requires extensive branching of epithelia via formation of cleft and buds. These branches are developed by local outgrowth or by formation of cleft that further divide epithelia in to buds. The bud or tubule extension is controlled and regulated by various growth factors. These branching finally led to formation of various organs. Cellular matrix protein fibronectin is required for salivary, lung and kidney branching (3). The edges of fibronectin translocates inwards as cleft accompanied by loss of E-cadherin in cells adjacent to fibronectin. The Btbd7 activity was noticed at cell cleft forming sites (2). The cleft formation involves buds delineation of buds by conversion of epithelial cell-cell adhesion to cell-matrix adhesion. Btbd7 provides a mechanistic link between the extracellular matrix and cleft propagation via its highly discrete expression that control epithelial cell motility by interaction with other cellular architectural and scaffolding proteins like Snail2 (slug), E-cadherin (4). The Btbd7 antibodies were generated using peptide corresponding to C-terminal domain of the human Btbd7 gene. The Btbd7 antibodies are affinity purified over immobilized antigen based affinity chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer. Antibodies to Btbd7 will label ~144kDa protein in Western blot positive control for Btbd7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-49652
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC-P, IP

Publications for BTBD7 Antibody (NBP1-49652)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(2)
WB
(2)
All Applications
Filter By Species
Human
(3)
Mouse
(2)
All Species
Showing Publications 1 - 9 of 9.
Publications using NBP1-49652 Applications Species
Yang L, Wang T, Zhang J, Wang X. BTBD7 silencing inhibited epithelial- mesenchymal transition (EMT) via regulating Slug expression in human salivary adenoid cystic carcinoma Cancer Biomark 2017 Sep 15 [PMID: 28946551] (Human) Human
Tao YM, Huang JL, Zeng S et al. BTB/POZ domain-containing protein 7: Epithelial-mesenchymal transition promoter and prognostic biomarker of hepatocellular carcinoma Hepatology 2013 Jan 16 [PMID: 23325674] (WB, IHC-P, Human, Mouse)

Details:
This citation used the FITC version of this antibody.
WB, IHC-P Human, Mouse
Onodera T, Sakai T, Hsu JC, Matsumoto K, Chiorini JA, Yamada KM. Btbd7 regulates epithelial cell dynamics and branching morphogenesis. Science;329(5991):562-5. 2010 Jul 30. [PMID: 20671187]
Dressler GR. The cellular basis of kidney development Annu Rev Cell Dev Biol 2006 [PMID: 16822174]

Details:
This citation used the FITC version of this antibody.
Pan W, Zhang Q, Xi QS, Gan RB, Li TP. FUP1, a gene associated with hepatocellular carcinoma, stimulates NIH3T3 cell proliferation and tumor formation in nude mice Biochem Biophys Res Commun 2001 Sep 7 [PMID: 11527404]

Details:
This citation used the FITC version of this antibody.
Nakamasu K, Kawamoto T, Yoshida E, Noshiro M, Matsuda Y, Kato Y. Structure and promoter analysis of the mouse membrane-bound transferrin-like protein (MTf) gene. Eur J Biochem;268(5):1468-76. 2001 Mar. [PMID: 11231300]
Dressler GR. The cellular basis of kidney development Annu Rev Cell Dev Biol Jun 28 1905

Details:
This publication used the FITC conjugated form of this antibody (Cat# NBP1-62182).
Pan W, Zhang Q, Xi QS et al. FUP1, a gene associated with hepatocellular carcinoma, stimulates NIH3T3 cell proliferation and tumor formation in nude mice Biochem Biophys Res Commun

Details:
This publication used the FITC conjugated form of this antibody (Cat# NBP1-62182).
Tao YM, Huang JL, Zeng S et al. BTB/POZ domain-containing protein 7: Epithelial-mesenchymal transition promoter and prognostic biomarker of hepatocellular carcinoma Hepatology (WB, IHC-P, Human, Mouse)

Details:
This publication used the FITC conjugated form of this antibody (Cat# NBP1-62182).
WB, IHC-P Human, Mouse

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Additional BTBD7 Products

BTBD7 NBP1-49652

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Bioinformatics

Gene Symbol BTBD7
Uniprot