BTBD7 Antibody

Western Blot: BTBD7 Antibody [NBP1-49652] - Human 293T cells were infected by lentivirus overexpression of control or BTBD7 for 72h. Total cell lysates were subjected to Western blot. PVDF membrane was probed with BTBD7 Antibody. A specific band was detected for BTBD7 at approximately 138 kDa. Image from verified customer review.

Western Blot: BTBD7 Antibody [NBP1-49652] - Lane A: Immune Serum, Lane B: Preimmune. Apparent MW is 138 kDa

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

BTBD7 Antibody Summary

• Immunogen: Synthetic peptide taken within amino acid region 1050-1150 on human BTBD7 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BTBD7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:200
  • Western Blot 1:5000
• Application Notes: Use in IHC-Paraffin reported in scientific literature (PMID: 23325674).
• Control Peptide:

  BTBD7 Protein (NBP1-49652PEP)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 11231300)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BTBD7 Antibody

• BTB (POZ) domain containing 7
• DKFZp686N0544
• FLJ10648
• FUP1BTB/POZ domain-containing protein 7
• KIAA1525
• MGC48310

Background

Cancer cells especially human hepatocellular carcinoma (HCC) differentially express many genes as determined by cDNA microarrays. One of these genes is Btbd7 whose function was not known. When transfected in NIH3T3 cells this gene exhibited enhanced cell proliferation activity by MTT assay. The enhanced tumorigencity was blocked by anti-sense RNA experiments (1). Recently it has been shown that Btbd7 regulate epithelial cell dynamics and branching morphogenesis. The organ development during embryogenesis requires extensive branching of epithelia via formation of cleft and buds. These branches are developed by local outgrowth or by formation of cleft that further divide epithelia in to buds. The bud or tubule extension is controlled and regulated by various growth factors. These branching finally led to formation of various organs. Cellular matrix protein fibronectin is required for salivary, lung and kidney branching (3). The edges of fibronectin translocates inwards as cleft accompanied by loss of E-cadherin in cells adjacent to fibronectin. The Btbd7 activity was noticed at cell cleft forming sites (2). The cleft formation involves buds delineation of buds by conversion of epithelial cell-cell adhesion to cell-matrix adhesion. Btbd7 provides a mechanistic link between the extracellular matrix and cleft propagation via its highly discrete expression that control epithelial cell motility by interaction with other cellular architectural and scaffolding proteins like Snail2 (slug), E-cadherin (4). The Btbd7 antibodies were generated using peptide corresponding to C-terminal domain of the human Btbd7 gene. The Btbd7 antibodies are affinity purified over immobilized antigen based affinity chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer. Antibodies to Btbd7 will label ~144kDa protein in Western blot positive control for Btbd7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BTBD7 Antibody (NBP1-49652)(8)

Publications using NBP1-49652

• Effects of Btbd7 knockdown on the proliferation of human dental pulp cells and expression of Dspp Bao Q, Zhang J, Wang XX J Clin Exp Pathol [PMID: 31938244] (IHC-P, Human)
• Liu Y, Song J, Zhang J et al. BTB/POZ domain-containing protein 7 is inversely associated with fibronectin expression in salivary adenoid cystic carcinoma. Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology 2017-12-28 [PMID: 29366608] (WB)
• Yang L, Wang T, Zhang J, Wang X. BTBD7 silencing inhibited epithelial- mesenchymal transition (EMT) via regulating Slug expression in human salivary adenoid cystic carcinoma Cancer Biomark 2017-09-15 [PMID: 28946551] (Human)
• Tao YM, Huang JL, Zeng S et al. BTB/POZ domain-containing protein 7: Epithelial-mesenchymal transition promoter and prognostic biomarker of hepatocellular carcinoma Hepatology 2013-01-16 [PMID: 23325674] (IHC-P, WB, Mouse, Human)
  
  Details:
  This citation used the FITC version of this antibody.
• Onodera T, Sakai T, Hsu JC, Matsumoto K, Chiorini JA, Yamada KM. Btbd7 regulates epithelial cell dynamics and branching morphogenesis. Science;329(5991):562-5. 2010-07-30 [PMID: 20671187]
• Dressler GR. The cellular basis of kidney development Annu Rev Cell Dev Biol 2006-01-01 [PMID: 16822174]
  
  Details:
  This citation used the FITC version of this antibody.
• Pan W, Zhang Q, Xi QS, Gan RB, Li TP. FUP1, a gene associated with hepatocellular carcinoma, stimulates NIH3T3 cell proliferation and tumor formation in nude mice Biochem Biophys Res Commun 2001-09-07 [PMID: 11527404]
  
  Details:
  This citation used the FITC version of this antibody.
• Nakamasu K, Kawamoto T, Yoshida E, Noshiro M, Matsuda Y, Kato Y. Structure and promoter analysis of the mouse membrane-bound transferrin-like protein (MTf) gene. Eur J Biochem;268(5):1468-76. 2001-03-01 [PMID: 11231300]

Review for BTBD7 Antibody (NBP1-49652) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 07/17/2018

Summary

• Application: Western Blot
• Sample Tested: 293T cell line
• Species: Human
• Lot: 2029

Comments

• Comments: 293T cells were infected by lentivirus overexpression of control or BTBD7 for 72h. Total cell lysates were subjected to western blot. PVDF membrane were probed with 1mm/ml Human BTBD7 Antibody. A specific band was detected for BTBD7 at approximately 138 kDa. This experiment was conducted under reducing conditions

Bioinformatics

• Gene Symbol: BTBD7
• Uniprot:
  • NP_001002860(Human)