Recombinant Human Biotinidase/BTD Protein

Product Discontinued

Recombinant Human Biotinidase/BTD Protein Summary

• Description: A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 44-545 of Human Biotinidase/BTD
  
  

  Source:Baculovirus

  Amino Acid Sequence: AHTGEESVAD HHEAEYYVAA VYEHPSILSL NPLALISRQE ALELMNQNLD IYEQQVMTAA QKDVQIIVFP EDGIHGFNFT RTSIYPFLDF MPSPQVVRWN PCLEPHRFND TEVLQRLSCM AIRGDMFLVA NLGTKEPCHS SDPRCPKDGR YQFNTNVVFS NNGTLVDRYR KHNLYFEAAF DVPLKVDLIT FDTPFAGRFG IFTCFDILFF DPAIRVLRDY KVKHVVYPTA WMNQLPLLAA IEIQKAFAVA FGINVLAANV HHPVLGMTGS GIHTPLESFW YHDMENPKSH LIIAQVAKNP VGLIGAENAT GETDPSHSKF LKILSGDPYC EKDAQEVHCD EATKWNVNAP PTFHSEMMYD NFTLVPVWGK EGYLHVCSNG LCCYLLYERP TLSKELYALG VFDGLHTVHG TYYIQVCALV RCGGLGFDTC GQEITEATGI FEFHLWGNFS TSYIFPLFLT SGMTLEVPDQ LGWENDHYFL RKSRLSSGLV TAALYGRLYE RDLEHHHHHH

• Source: Baculovirus
• Protein/Peptide Type: Recombinant Protein
• Gene: BTD
• Purity: >85%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 57.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% glycerol
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human Biotinidase/BTD Protein

• Biotinase
• Biotinidase
• BTD
• EC 3.5.1
• EC 3.5.1.12

Background

Biotinidase functions to recycle biotin in the body by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. Biotinidase has also been shown to have biotinyl-transferase activity. Defects in the biotinidase gene cause multiple carboxylase deficiency. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: BTD