beta-Arrestin 2 Antibody

Western Blot: beta-Arrestin 2 Antibody [NB100-1515] - Staining of A549 (A),and U251 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: beta-Arrestin 2 Antibody [NB100-1515] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing plasma membrane, cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Flow Cytometry: beta-Arrestin 2 Antibody [NB100-1515] - Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

beta-Arrestin 2 Antibody Summary

• Description: Novus Biologicals Goat beta-Arrestin 2 Antibody (NB100-1515) is a polyclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. Anti-beta-Arrestin 2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-HDHIPLPRPQS corresponding to internal region according to NP_004304.1, NP_945355.1.
• Specificity: This antibody is expected to recognise both reported isoforms 1, 2, 3, 5 and 6 (NP_004304.1; NP_945355.1; NP_001244257.1; NP_001244259.1; NP_001244260.1). No crossreactivity is expected with Arrestin beta 1.
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ARRB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.5 - 1 ug/mL

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for beta-Arrestin 2 Antibody

• ARB2
• ARR2
• ARRB2
• arrestin 3
• Arrestin beta-2
• arrestin, beta 2
• BARR2
• betaArrestin 2
• beta-Arrestin 2
• beta-arrestin-2
• DKFZp686L0365

Background

Members of arrestin/beta arrestin protein family are thought to participate in agonist mediated desensitization of G protein coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-Arrestin 2 Antibody (NB100-1515)(2)

Publications using NB100-1515

• Zakrzewicz A, Krasteva G, Wilhelm J et al. Reduced expression of arrestin beta 2 by graft monocytes during acute rejection of rat kidneys. Immunobiology 2011-07-01 [PMID: 21193245]
• Lefkowitz RJ, Shenoy SK. Transduction of receptor signals by beta-arrestins. Science 2005-04-22 [PMID: 15845844]

Bioinformatics

• Gene Symbol: ARRB2
• Entrez:
  • 409(Human)
• Uniprot:
  • NP_004304.1(Human)