beta-Arrestin 1 Antibody (ST51-08)

Western Blot: beta-Arrestin 1 Antibody (ST51-08) [NBP2-67510] - Western blot analysis of beta-Arrestin 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goa

Immunocytochemistry/Immunofluorescence: beta-Arrestin 1 Antibody (ST51-08) [NBP2-67510] - Beta Arrestin 1 in HeLa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100 in PBS.

Immunohistochemistry-Paraffin: beta-Arrestin 1 Antibody (ST51-08) [NBP2-67510] - Analysis of paraffin-embedded mouse lung tissue using anti-beta Arrestin 1 antibody. Counter stained with hematoxylin.

Flow Cytometry: beta-Arrestin 1 Antibody (ST51-08) [NBP2-67510] - Analysis of Hela cells with beta Arrestin 1 antibody at 1:50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: beta-Arrestin 1 Antibody (ST51-08) [NBP2-67510] - Analysis of MIN6 and mouse pancreatic islets. Lane 1: Molecular Weight Ladder. Lanes 2-3: MIN6 cells (~10 ug). Lane 4: pooled wild-type pancreatic islets (~5 ug). Lane 5: pooled beta-arrestin 1 knockout pancreatic islets (~5 ug). Primary Antibody: Anti-beta-arrestin 1 Ab at 1:750 overnight at 4C. Secondary Antibody: Goat Anti-rabbit IgG: HRP at 1:3000 for 1hr RT. Image from verified customer review.

Western Blot: beta-Arrestin 1 Antibody (ST51-08) [NBP2-67510] - Analysis of beta Arrestin 1 on different lysates using anti-beta Arrestin 1 antibody at 1:1,000 dilution. Positive control: Lane 1: PC12 Lane 2: Jurkat.

Immunocytochemistry/Immunofluorescence: beta-Arrestin 1 Antibody (ST51-08) [NBP2-67510] - Beta Arrestin 1 in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100 in PBS.

Immunocytochemistry/Immunofluorescence: beta-Arrestin 1 Antibody (ST51-08) [NBP2-67510] - Beta Arrestin 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100i n PBS.

Immunohistochemistry-Paraffin: beta-Arrestin 1 Antibody (ST51-08) [NBP2-67510] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-beta Arrestin 1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: beta-Arrestin 1 Antibody (ST51-08) [NBP2-67510] - Analysis of paraffin-embedded human liver cancer tissue using anti-beta Arrestin 1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: beta-Arrestin 1 Antibody (ST51-08) [NBP2-67510] - Analysis of paraffin-embedded human lung tissue using anti-beta Arrestin 1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: beta-Arrestin 1 Antibody (ST51-08) [NBP2-67510] - Analysis of paraffin-embedded mouse brain tissue using anti-beta Arrestin 1 antibody. Counter stained with hematoxylin.

Product Discontinued

beta-Arrestin 1 Antibody (ST51-08) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human beta-Arrestin 1 aa 1-50 / 418. (SwissProt: P49407 Human; SwissProt: Q8BWG8 Mouse; SwissProt: P29066 Rat)
• Localization: Cell membrane, Cell projection, Coated pit, Cytoplasm, Cytoplasmic vesicle, Membrane, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ARRB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation
  • Knockout Validated
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for beta-Arrestin 1 Antibody (ST51-08)

• ARB1
• ARR1
• ARRB1
• arrestin 2
• Arrestin beta-1
• arrestin, beta 1
• betaArrestin 1
• beta-Arrestin 1
• beta-arrestin-1

Background

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization ofG-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones,neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in thebeta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the centralnervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin systemis believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcriptsencoding different isoforms of arrestin beta 1 have been described, however, their exact functions are not known.(provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for beta-Arrestin 1 Antibody (NBP2-67510) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 04/22/2019

Summary

• Application: Western Blot
• Sample Tested: Min6 mouse insulinoma whole cell lysate,Mouse Pancreatic islet,Mouse Pancreatic islets
• Species: Mouse
• Lot: HJ0307

Comments

• Comments: Lane 1: Molecular Weight Ladder. Lanes 2-3: Samples from MIN6 cells (Load: ~10ug). Lane 4: pooled wild-type pancreatic islets (load: ~5ug). Lane 5: pooled beta-arrestin 1 knockout pancreatic islets (load: ~5ug). Primary Antibody: Anti-beta-arrestin 1 Ab at 1:750 overnight at 4C. Secondary Antibody: Goat Anti-rabbit IgG: HRP at 1:3000 for 1hr RT.

Bioinformatics

• Gene Symbol: ARRB1