BAP28 Antibody - BSA Free

Western Blot: BAP28 Antibody [NBP3-12652] - Lane A: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:242 kDaObserved band size:242 kDa

Immunohistochemistry-Paraffin: BAP28 Antibody [NBP3-12652] - Staining of human HEATR1 in human adrenal gland with rabbit polyclonal antibody at 1:300 dilution.

Immunohistochemistry-Paraffin: BAP28 Antibody [NBP3-12652] - Staining of human HEATR1 in human kidney with rabbit polyclonal antibody at 1:300 dilution.

Immunoprecipitation: BAP28 Antibody [NBP3-12652] - Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-HEATR1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-HEATR1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 242 kDaObserved band size :242 kDa

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

BAP28 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
  
  Protein A & Antigen Affinity purified
• Immunogen: Produced in rabbits immunized with E. coli-derived Human BAP28 fragment
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HEATR1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for BAP28 Antibody - BSA Free

• BAP28HEAT repeat-containing protein 1
• EC 4.1.1.31
• EC 6.3.4.4
• FLJ10359
• HEAT repeat containing 1
• MGC72083
• Protein BAP28

Background

IRAK-4 is a member of the interleukine-1 receptor-associated kinase (IRAK) family. The IRAK family is implicated in the Toll-like receptor (TLR) and Il-1R signaling pathway (1). Upon phosphorylation, IRAK-4 interacts with IRAK-1, MYD88 and TRAF6 to form a complex, and activate NF-kB and MAPK pathways (2-3). IRAK-4 phosphorylates and activates IRAK-1 at thr387 and is also known to interact with IRAK-1 but not with the other IRAK members. Additionally, like IRAK-1, IRAK-4 possesses an auto and cross-phosphorylation kinase activity (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HEATR1