AP3M1 Antibody - BSA Free

Western Blot: AP3M1 Antibody [NBP1-76589] - Analysis of AP3M1 in human brain tissue lysate with AP3M1 antibody at (A) 1 and (B) 2 ug/mL .

Immunohistochemistry: AP3M1 Antibody - BSA Free [NBP1-76589] - Immunohistochemistry of AP3M1 in mouse brain tissue with AP3M1 antibody at 5 ug/mL.

Immunocytochemistry/ Immunofluorescence: AP3M1 Antibody - BSA Free [NBP1-76589] - Immunofluorescence of AP3M1 in mouse brain tissue with AP3M1 antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt, Bv, Ch
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

AP3M1 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human AP3M1. The immunogen is located within amino acids 340 - 390 of AP3M1. Amino Acid Squence: VNLQSGAPKPEENPSLN
• Specificity: AP3M1 antibody may cross-react with AP3M2.
• Predicted Species: Bovine (100%), Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AP3M1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-2 ug/ml
• Theoretical MW: 47s kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  AP3M1 Peptide (NBP1-76589PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for AP3M1 Antibody - BSA Free

• adaptor-related protein complex 3, mu 1 subunit
• AP-3 adapter complex mu3A subunit
• MGC22164
• mu-3A subunit
• mu3A-adaptin

Background

The AP3 complex is a heterotetramer composed of two large adaptins (AP3D1, AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. AP3M1 is highly homologous to AP3M2 but is the preferred binding partner to the YQRL motif from the cytosolic domain of TGN38. AP3M1 has also been shown to interact with the HIV-1 virulence protein Nef.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AP3M1
• Entrez:
  • 26985(Human)
• Uniprot:
  • Q9Y2T2(Human)