AP2S1 Overexpression Lysate

Western Blot: AP2S1 Overexpression Lysate (Adult Normal) [NBL1-07582] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AP2S1.

• Reactivity: Hu
• Applications: WB

AP2S1 Overexpression Lysate Summary

• Description:

  AP2S1 Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC202919
  
  Accession#: NM_004069
  
  Protein Tag: C-MYC/DDK
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
• Gene: AP2S1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
  
  Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
• Theoretical MW: 16.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for AP2S1 Overexpression Lysate

• Adapter-related protein complex 2 sigma subunit
• Adaptor protein complex AP-2 subunit sigma
• adaptor-related protein complex 2, sigma 1 subunit
• AP17HA2 17 kDa subunit
• AP-2 complex subunit sigma
• CLAPS2AP17-DELTA
• Clathrin assembly protein 2 small chain
• Clathrin coat assembly protein AP17
• Clathrin coat-associated protein AP17
• clathrin-associated/assembly/adaptor protein, small 2 (17kD)
• Plasma membrane adaptor AP-2 17 kDa protein
• sigma2-adaptin

Background

One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this complex. Alternative splicing has been observed in this gene and results in two known transcripts. Transcript Variant: This variant (AP17) encodes the longer isoform (AP17).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: AP2S1