Angiopoietin-like protein 8/Betatrophin Antibody (910248) [Unconjugated]

Angiopoietin-like Protein 8/Betatrophin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Angiopoietin-like Protein 8/Betatrophin Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB8548) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western blot shows lysates of CHO Chinese hamster ovary cell line and HEK293 human embryonic kidney cell line either mock transfected or transfected with human Angiopoietin-like Protein 8 (ANGPTL8)/Betatrophin. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Angiopoietin-like Protein 8/Betatrophin Antigen Affinity-purified Polyclonal Antibody (Catalog # MAB8548) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Angiopoietin-like Protein 8/Betatrophin at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Angiopoietin-like Protein 8/Betatrophin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Angiopoietin-like Protein 8/Betatrophin Monoclonal Antibody (Catalog # MAB8548) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clone: 910248
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Angiopoietin-like protein 8/Betatrophin Antibody (910248) [Unconjugated] Summary

• Immunogen: Human Angiopoietin-like Protein 8/Betatrophin synthetic peptide with sequences corresponding to the N-term region
  Accession # Q6UXH0
• Specificity: Detects human Angiopoietin-like Protein 8/Betatrophin in direct ELISA and Western Blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANGPTL8
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiopoietin-like protein 8/Betatrophin Antibody (910248) [Unconjugated]

• Angiopoietin like Protein 8
• Angiopoietin-like Protein 8
• ANGPTL8
• Betatrophin
• C19orf80
• chromosome 19 open reading frame 80
• Gm6484
• hepatocellular carcinoma-associated gene TD26
• hepatocellular carcinoma-associated protein TD26
• Lipasin
• PRO1185
• PVPA599
• RIFL
• TD26
• UNQ599

Background

Betatrophin also known as C19orf80, ANGPTL8, and TD26, is a secreted hormone protein.  It is 198 amino acids (aa) in length, and shares 72% aa identity with mouse Betatrophin.  It is primarily expressed in the liver and adipose tissue and has been shown to be increased in the plasma of diabetic patients.  Additionally, Betatrophin has been demonstrated to increase the proliferation of beta cells in the pancreas of mice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Angiopoietin-like protein 8/Betatrophin Antibody (MAB8548)(1)

Publication using MAB8548

• H Arefanian, I Al-Khairi, NA Khalaf, P Cherian, S Kavalakatt, D Madhu, A Mathur, MG Qaddoumi, F Al-Mulla, J Abubaker, M Abu-Farha Increased expression level of ANGPTL8 in white adipose tissue under acute and chronic cold treatment Lipids in Health and Disease, 2021-09-26;20(1):117. 2021-09-26 [PMID: 34565390] (IHC, Mouse)

Bioinformatics

• Gene Symbol: ANGPTL8
• Uniprot:
  • Q6UXH0()