Amiloride-sensitive cation channel 3 Antibody

Images

 
Immunofluorescence: Amiloride-sensitive cation channel 3 Antibody [NBP1-20095] - Rat hypothalamus stained with anti-Amiloride-sensitive cation channel 3 antibody. Image from verified customer review.

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Amiloride-sensitive cation channel 3 Antibody Summary

Immunogen
A synthetic peptide from the extracellular domain of rat amiloride sensitive cation channel 3 (ACCN3, ASIC3, SLNAC1, TNAC1) conjugated to immunogenic carrier protein was used as the immunogen. The antigen is homologous in mouse.
Specificity
ASIC3.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ASIC3
Purity
Unpurified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:300-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Reviewed Applications
Read 1 Review rated 4
using
NBP1-20095 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Concentration
LYOPH
Purity
Unpurified
Reconstitution Instructions
Reconstitute with 0.1 ml sterilized water to desired concentration.

Alternate Names for Amiloride-sensitive cation channel 3 Antibody

  • Acid-sensing ion channel 3
  • amiloride-sensitive cation channel 3
  • amiloride-sensitive cation channel 3, testis
  • ASIC3hTNaC1
  • DRASIC
  • hASIC3
  • modulatory subunit of ASIC2a
  • Neuronal amiloride-sensitive cation channel 3
  • proton-gated cation channel subunit
  • SLNAC1
  • Testis sodium channel 1
  • TNaC1

Background

This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, two hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is an acid sensor and may play an important role in the detection of lasting pH changes. In addition, a heteromeric association between this member and ACCN1 has been observed as proton-gated channels sensitive to gadolinium. Alternative splicing of this gene generates three transcript variants encoding distinct isoforms.; FUNCTION: Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase. In sensory neurons is proposed to mediate the pain induced by acidosis that occurs in ischemic, damaged or inflamed tissue. May be involved in hyperalgesia. May play a role in mechanoreception. Heteromeric channel assembly seems to modulate channel properties.; SUBUNIT: Homotetramer or heterotetramer with other ASIC proteins (Probable). Interacts with STOM and DLG4. Interacts with LIN7B, MAGI1/BAIAP1, GOPC and ACCN1.; SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cytoplasm. Note: Cell surface expression may be stabilized by interaction with LIN7B and cytoplasmic retention by interaction with DLG4. In part cytoplasmic in cochlea cells.; TISSUE SPECIFICITY: Expressed by sensory neurons. Strongly expressed in brain, spinal chord, lung, lymph nodes, kidney, pituitary, heart and testis.; DEVELOPMENTAL STAGE: Expressed in fetal tissues, expression increases in lung and kidney adult tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for Amiloride-sensitive cation channel 3 Antibody (NBP1-20095) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.
We have 1 review tested in 1 application: IF.

Reviews using NBP1-20095:
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Images Ratings Applications Species Date Details
Immunofluorescence Amiloride-sensitive cation channel 3 NBP1-20095
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reviewed by:
Chris Holton
IF Rat 07/08/2016
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Summary

ApplicationImmunofluorescence
Sample Testedrat hypothalamus
SpeciesRat
LotRB0714-22108-WS

Blocking

Blocking DetailsBlocked 10% goat serum

Primary Anitbody

Dilution Ratio1:100 NBP1-20095 overnight in free floating 30um sections.

Secondary Antibody

Secondary DescriptionBiotinylated goat anti rabbit 2ndary plus A/B conjugate plus Flurocein Avidin D plus Tyramide amplification
Secondary Concentration1:500 each antibody, 1:100 tyramide 5 minutes

Details

Detection NotesFluorcein
Fixation Details4% paraformaldehyde perfusion-fixed. Sliced at 30um
Wash DescriptionPBS with 0.1% triton X

Comments

CommentsWorks without Tyramide amplification and at a slightly lower conc primary too.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Recent Reviews

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1
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Chris Holton
07/08/2016
Application: IF
Species: Rat

Bioinformatics

Gene Symbol ASIC3
Entrez
Uniprot