AR013 and
AR003, respectively) to select and enrich for neural stem cell populations, and a panel of growth factors for effective dopaminergic differentiation, including Recombinant Human FGF-basic, Recombinant Mouse FGF-8b (
423-F8), and Recombinant Mouse Shh-N (
464-SH). Cells were stained with a Mouse Anti-Human/Mouse Tyrosine Hydroxylase Monoclonal Antibody (
MAB7566) followed by a NorthernLights™ 493-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (
NL009; green), and a Mouse Neuron-specific beta III Tubulin Tuj1 Monoclonal Antibody (
MAB1195) followed by a NorthernLights 557-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (
NL007; red) and counterstained with DAPI (
5748; blue). " class="big_lightbox">
AR013 and
AR003, respectively) to select and enrich for neural stem cell populations, and a panel of growth factors for effective dopaminergic differentiation, including Recombinant Human FGF-basic, Recombinant Mouse FGF-8b (
423-F8), and Recombinant Mouse Shh-N (
464-SH). Cells were stained with a Mouse Anti-Human/Mouse Tyrosine Hydroxylase Monoclonal Antibody (
MAB7566) followed by a NorthernLights™ 493-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (
NL009; green), and a Mouse Neuron-specific beta III Tubulin Tuj1 Monoclonal Antibody (
MAB1195) followed by a NorthernLights 557-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (
NL007; red) and counterstained with DAPI (
5748; blue). " alt="Dopaminergic neurons were generated from human pluripotent stem cells in media that included Bovine Fibronectin Protein (Catalog # 1030-FN) to support cell attachment and spreading, the ITS and N-2 Plus Media Supplements (
AR013 and
AR003, respectively) to select and enrich for neural stem cell populations, and a panel of growth factors for effective dopaminergic differentiation, including Recombinant Human FGF-basic, Recombinant Mouse FGF-8b (
423-F8), and Recombinant Mouse Shh-N (
464-SH). Cells were stained with a Mouse Anti-Human/Mouse Tyrosine Hydroxylase Monoclonal Antibody (
MAB7566) followed by a NorthernLights™ 493-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (
NL009; green), and a Mouse Neuron-specific beta III Tubulin Tuj1 Monoclonal Antibody (
MAB1195) followed by a NorthernLights 557-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (
NL007; red) and counterstained with DAPI (
5748; blue). " class="big_thumb" />
AR013 and
AR003, respectively), and a panel of growth factors for effective dopaminergic differentiation, including Recombinant Human FGF-basic, Recombinant Mouse FGF-8b (
423-F8), and Recombinant Mouse Shh-N (
464-SH). Tyrosine Hydroxylase was detected using a Mouse Anti-Human Tyrosine Hydroxylase Monoclonal Antibody (
MAB7566). The cells were stained with the NorthernLights™ 557-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (
NL007; red). Neuron-specific beta-III Tubulin was detected using a Mouse Anti-Neuron-specific beta-III Tubulin (Clone Tuj-1) Monoclonal Antibody (
MAB1195) followed by the NorthernLights™ 493-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (
NL009; green). Cells were counterstained with DAPI (
5748; blue)." class="big_lightbox">
AR013 and
AR003, respectively), and a panel of growth factors for effective dopaminergic differentiation, including Recombinant Human FGF-basic, Recombinant Mouse FGF-8b (
423-F8), and Recombinant Mouse Shh-N (
464-SH). Tyrosine Hydroxylase was detected using a Mouse Anti-Human Tyrosine Hydroxylase Monoclonal Antibody (
MAB7566). The cells were stained with the NorthernLights™ 557-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (
NL007; red). Neuron-specific beta-III Tubulin was detected using a Mouse Anti-Neuron-specific beta-III Tubulin (Clone Tuj-1) Monoclonal Antibody (
MAB1195) followed by the NorthernLights™ 493-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (
NL009; green). Cells were counterstained with DAPI (
5748; blue)." class="big_thumb" />
AR013 and
AR003), and a panel of growth factors for effective oligodendrocyte differentiation, including Recombinant Human FGF-basic, Recombinant Human EGF (
236-EG), and Recombinant Human PDGF-AA (
221-AA). Oligodendrocytes were detected using a Mouse Anti-Human/Mouse/Rat/Chicken Oligodendrocyte Marker O4 Monoclonal Antibody (
MAB1326). The cells were stained with the NorthernLights™-557 Affinity-purified Goat Anti-Mouse IgM Secondary Antibody (
NL019; red). The nuclei were counterstained with DAPI (
5748;blue). " class="big_lightbox">
AR013 and
AR003), and a panel of growth factors for effective oligodendrocyte differentiation, including Recombinant Human FGF-basic, Recombinant Human EGF (
236-EG), and Recombinant Human PDGF-AA (
221-AA). Oligodendrocytes were detected using a Mouse Anti-Human/Mouse/Rat/Chicken Oligodendrocyte Marker O4 Monoclonal Antibody (
MAB1326). The cells were stained with the NorthernLights™-557 Affinity-purified Goat Anti-Mouse IgM Secondary Antibody (
NL019; red). The nuclei were counterstained with DAPI (
5748;blue). " class="big_thumb" />
2604-FG) induces proliferation in BaF3 mouse pro-B cells transfected with human FGFR3 (IIIc). The ED50 for this effect is 0.1-0.4 μg/mL in the presence of recombinant mouse Klotho (1819-KL) and heparin." alt="Recombinant human FGF-23 (2604-FG) induces proliferation in BaF3 mouse pro-B cells transfected with human FGFR3 (IIIc). The ED50 for this effect is 0.1-0.4 μg/mL in the presence of recombinant mouse Klotho (1819-KL) and heparin." class="big_thumb" />
1324-WN)." alt="Recombinant R-Spondin 2 (Catalog # 3266-RS) induces activation of beta -catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is 0.7-2.8 ng/mL in the presence of 5 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN)." class="big_thumb" />
AR013 and AR003, respectively) to select and enrich for neural stem cell populations, and a panel of growth factors for effective dopaminergic differentiation, including Recombinant Human FGF-basic, Recombinant Mouse FGF-8b (423-F8), and Recombinant Mouse Shh-N (464-SH). Cells were stained with a Mouse Anti-Human/Mouse Tyrosine Hydroxylase Monoclonal Antibody (MAB7566) followed by a NorthernLights™ 493-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (NL009; green), and a Mouse Neuron-specific beta III Tubulin Tuj1 Monoclonal Antibody (MAB1195) followed by a NorthernLights 557-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (NL007; red) and counterstained with DAPI (5748; blue). " alt="Dopaminergic neurons were generated from human pluripotent stem cells in media that included Bovine Fibronectin Protein (Catalog # 1030-FN) to support cell attachment and spreading, the ITS and N-2 Plus Media Supplements (AR013 and AR003, respectively) to select and enrich for neural stem cell populations, and a panel of growth factors for effective dopaminergic differentiation, including Recombinant Human FGF-basic, Recombinant Mouse FGF-8b (423-F8), and Recombinant Mouse Shh-N (464-SH). Cells were stained with a Mouse Anti-Human/Mouse Tyrosine Hydroxylase Monoclonal Antibody (MAB7566) followed by a NorthernLights™ 493-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (NL009; green), and a Mouse Neuron-specific beta III Tubulin Tuj1 Monoclonal Antibody (MAB1195) followed by a NorthernLights 557-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (NL007; red) and counterstained with DAPI (5748; blue). " class="big_thumb" />
ES001)." alt="Recombinant Mouse MMP-9 (Catalog # 909-MM) is measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (Catalog # 
AR013 and 
AR013 and 
