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![Western Blot: Mre11-Rad50-NBS1 Complex Antibody Pack [NB100-914] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and Nbs1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human Polyclonal [NB100-143] followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific band was detected for Nbs1 at approximately 95 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Mre11-Rad50-NBS1-Complex-Antibody-Pack-Knockout-Validated-NB100-914-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Mre11-Rad50-NBS1 Complex Antibody Pack [NB100-914] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and Nbs1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human Polyclonal [NB100-143] followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific band was detected for Nbs1 at approximately 95 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Simple Western: Mre11-Rad50-NBS1 Complex Antibody Pack [NB100-914] - Image shows a specific band for Mre11 in 0.5 mg/mL of HeLa lysate using NB100-142. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Mre11-Rad50-NBS1-Complex-Antibody-Pack-Simple-Western-NB100-914-img0008.jpg)
![Simple Western: Mre11-Rad50-NBS1 Complex Antibody Pack [NB100-914] - Simple Western lane view shows a specific band for Rad50 in 0.5 mg/ml of HeLa lysate using NB100-154. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Mre11-Rad50-NBS1-Complex-Antibody-Pack-Simple-Western-NB100-914-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Mre11-Rad50-NBS1 Complex Antibody Pack [NB100-914] - ICC/IF analysis of Nbs1 in HeLa cells with Nbs1 Antibody [NB100-143]. Image from verified customer review.](http://images.novusbio.com/fullsize/Mre11-Rad50-NBS1-Complex-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-914-img0011.jpg)
![Immunohistochemistry-Paraffin: Mre11-Rad50-NBS1 Complex Antibody Pack [NB100-914] - FFPE human testis using Nbs1 antibody [NB100-143] at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Mre11-Rad50-NBS1-Complex-Antibody-Pack-Immunohistochemistry-Paraffin-NB100-914-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Mre11-Rad50-NBS1 Complex Antibody Pack [NB100-914] - Staining of Mre11 using NB100-142 on HeLa cells. Image from verified customer review.](http://images.novusbio.com/fullsize/Mre11-Rad50-NBS1-Complex-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-914-img0010.jpg)
![Immunohistochemistry: Mre11-Rad50-NBS1 Complex Antibody Pack [NB100-914] - MRE11 staining using NB100-142 in the human epidermis detected.](http://images.novusbio.com/fullsize/Mre11-Rad50-NBS1-Complex-Antibody-Pack-Immunohistochemistry-NB100-914-img0005.jpg)
![Immunohistochemistry-Paraffin: TERT Antibody Pack [NB100-913] - TERT was detected in immersion fixed paraffin-embedded sections of human liver cancer using anti-human mouse monoclonal antibody (Catalog # NB100-297) at 1:600 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/TERT-Antibody-Pack-Immunohistochemistry-Paraffin-NB100-913-img0011.jpg "Immunohistochemistry-Paraffin: TERT Antibody Pack [NB100-913] - TERT was detected in immersion fixed paraffin-embedded sections of human liver cancer using anti-human mouse monoclonal antibody (Catalog # NB100-297) at 1:600 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.")
![Western Blot: TERT Antibody Pack [NB100-913] - Increased levels of IFI16 protein in WI-38 cells reduce hTERT levels and inhibit telomerase activity. Total protein extracts prepared from WI-38 cells, either transfected with control (pCMV) vector (lane 1) or pCMV-IFI16 plasmid (lane 2), were subjected to immunoblotting using antibodies specific to the indicated proteins. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0008569), licensed under a CC-BY license. TERT Antibody (2C4) [NB100-317]](http://images.novusbio.com/fullsize/TERT-Antibody-Pack-Western-Blot-NB100-913-img0010.jpg)
![Immunohistochemistry-Paraffin: TERT Antibody Pack [NB100-913] - Normal human pancreas showing moderate TERT staining of exocrine cells and a subset of islets of Langerhans using TERT antibody (NB100-317).](http://images.novusbio.com/fullsize/TERT-Antibody-Pack-Immunohistochemistry-Paraffin-NB100-913-img0009.jpg)
![Beclin 1 Antibody Pack [NB910-95610] - Lane view shows a specific band for Beclin in 1.0 mg/ml of HeLa lysate using NB110-87318. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-NB910-95610-img0014.jpg "Beclin 1 Antibody Pack [NB910-95610] - Lane view shows a specific band for Beclin in 1.0 mg/ml of HeLa lysate using NB110-87318. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![<b>Orthogonal Strategies Validation. </b>Beclin 1 Antibody Pack [NB910-95610] - Expression of Beclin 1 mRNA and protein in cells and tissues. (A) Beclin 1 mRNA expression level up-regulated in gastric cancer cell lines compared with normal gastric epithelial cells by reverse transcription-PCR. (B) Beclin 1 protein expression level up-regulated in gastric cancer cell lines compared with normal gastric epithelial cells by western blotting. (C) Beclin 1 mRNA expression is elevated in primary gastric tumors compared with paired gastric adjacent noncancerous tissues by reverse transcription-PCR. (D) Beclin 1 protein expression is elevated in primary gastric tumors compared with paired gastric adjacent noncancerous tissues by western blotting. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0045968) licensed under a CC-BY license. Anti-Beclin-1 Antibody NB500-249](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-NB910-95610-img0013.jpg)
![Immunohistochemistry: Beclin 1 Antibody Pack [NB910-95610] - Analysis of a FFPE tissue section of human breast cancer using Beclin 1 antibody (NB110-87318) at 1:300 dilution. The primary antibody bound to Beclin 1 antigen in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. Cytoplasmic staining can be seen in the cancer as well as the stromal cells. The signal was strongest in a subset of stromal cells which appears to be the cancer associated fibroblasts.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Immunohistochemistry-NB910-95610-img0018.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Beclin 1 Antibody Pack [NB910-95610] - siRNA knockdown of becn1 (Beclin 1). Lysates from OKP7 cells treated with non-targeting siRNA (non-target) or an siRNA pool directed against Beclin 1 (becn1) were analyzed by Western blot for Beclin 1 production using NB500-249. GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.ppat.1003394) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Knockdown-Validated-NB910-95610-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Beclin 1 Antibody Pack [NB910-95610] - Beclin 1 antibody (NB500-249) was tested in HeLa cells with Dylight 488 (green, left panel). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red), shown in the overlay (right panel)](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB910-95610-img0020.jpg)
![Immunohistochemistry: Beclin 1 Antibody Pack [NB910-95610] - Detecting Beclin 1 using NB500-249 in Pheochromocytes of the Adrenal Medulla at 40x.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Immunohistochemistry-NB910-95610-img0011.jpg)
![Western Blot: Beclin 1 Antibody Pack [NB910-95610] - Analysis of Beclin1 using NB500-249. Lane 1: human brain. Lane 2: mouse brain.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Western-Blot-NB910-95610-img0015.jpg)
![Western Blot: Beclin 1 Antibody Pack [NB910-95610] - Detection of Beclin 1 in liver lysates using NB 500-266. Lane 1: mouse liver Lane 2: human liver.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Western-Blot-NB910-95610-img0012.jpg)
![Simple Western: Beclin 1 Antibody Pack [NB910-95609] - Lane view shows a specific band for Beclin in 1.0 mg/ml of HeLa lysate using NB110-87318. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Simple-Western-NB910-95609-img0005.jpg "Simple Western: Beclin 1 Antibody Pack [NB910-95609] - Lane view shows a specific band for Beclin in 1.0 mg/ml of HeLa lysate using NB110-87318. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Beclin 1 Antibody Pack [NB910-95609] - Expression of Beclin 1 mRNA and protein in cells and tissues. (A) Beclin 1 mRNA expression level up-regulated in gastric cancer cell lines compared with normal gastric epithelial cells by reverse transcription-PCR. (B) Beclin 1 protein expression level up-regulated in gastric cancer cell lines compared with normal gastric epithelial cells by western blotting. (C) Beclin 1 mRNA expression is elevated in primary gastric tumors compared with paired gastric adjacent noncancerous tissues by reverse transcription-PCR. (D) Beclin 1 protein expression is elevated in primary gastric tumors compared with paired gastric adjacent noncancerous tissues by western blotting. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0045968) licensed under a CC-BY license. Anti-Beclin-1 Antibody NB500-249](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Western-Blot-NB910-95609-img0013.jpg)
![Immunohistochemistry: Beclin 1 Antibody Pack [NB910-95609] - Analysis of a FFPE tissue section of human breast cancer using Beclin 1 antibody (NB110-87318) at 1:300 dilution. The primary antibody bound to Beclin 1 antigen in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. Cytoplasmic staining can be seen in the cancer as well as the stromal cells. The signal was strongest in a subset of stromal cells which appears to be the cancer associated fibroblasts.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Immunohistochemistry-NB910-95609-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Beclin 1 Antibody Pack [NB910-95609] - siRNA knockdown of becn1 (Beclin 1). Lysates from OKP7 cells treated with non-targeting siRNA (non-target) or an siRNA pool directed against Beclin 1 (becn1) were analyzed by Western blot for Beclin 1 production using NB500-249. GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.ppat.1003394) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Knockdown-Validated-NB910-95609-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Beclin 1 Antibody Pack [NB910-95609] - Beclin 1 antibody (NB500-249) was tested in HeLa cells with Dylight 488 (green, left panel). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red), shown in the overlay (right panel)](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB910-95609-img0010.jpg)
![Beclin 1 Antibody Pack [NB910-95609] - Detecting Beclin 1 using NB500-249 in Pheochromocytes of the Adrenal Medulla at 40x.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-NB910-95609-img0011.jpg)
![Western Blot: Beclin 1 Antibody Pack [NB910-95609] - Detection of Beclin 1 in liver lysates using NB500-266. (1) mouse liver and (2) human liver.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Western-Blot-NB910-95609-img0014.jpg)
![Western Blot: Beclin 1 Antibody Pack [NB910-95609] - Analysis of Beclin1 using NB500-249. Lane 1: human brain. Lane 2: mouse brain.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Western-Blot-NB910-95609-img0006.jpg)
![Western Blot: ER Stress / UPR Antibody Pack [NBP2-52746] - Hepatic ER stress markers with cachexia progression. ER stress markers Bip, IRE1a, ATF6p50 and CHOP were examined in the liver of non, pre and severely cachectic mice. (n = 6-8 per group, p < 0.05) Dotted line indicates levels of Non-cachectic mice. Non = Non-Cachectic Apc Min/+ Sev = severely cachectic Apc Min/+; * denotes significantly different from Non-cachectic Apc Min/+ Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0119888) licensed under a CC-BY license. IRE1 alpha [p Ser724] Antibody [NB100-2323]](http://images.novusbio.com/fullsize/ER-Stress---UPR-Antibody-Pack-Western-Blot-NBP2-52746-img0007.jpg "Western Blot: ER Stress / UPR Antibody Pack [NBP2-52746] - Hepatic ER stress markers with cachexia progression. ER stress markers Bip, IRE1a, ATF6p50 and CHOP were examined in the liver of non, pre and severely cachectic mice. (n = 6-8 per group, p < 0.05) Dotted line indicates levels of Non-cachectic mice. Non = Non-Cachectic Apc Min/+ Sev = severely cachectic Apc Min/+; * denotes significantly different from Non-cachectic Apc Min/+ Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0119888) licensed under a CC-BY license. IRE1 alpha [p Ser724] Antibody [NB100-2323]")
![Immunohistochemistry: ER Stress / UPR Antibody Pack [NBP2-52746] - XBP1 and TG2 deficiency promote increased IRE1a protein levels in cultured VSMCs and in neointima in situ. (D) Xbp1WT and Xbp1 delta WT and (E) Tgm2 WT and Tgm2 delta carotid artery sections were analyzed by IHC for total IRE1a. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0212235) licensed under a CC-BY license. IRE1 alpha Antibody [NB100-2324]](http://images.novusbio.com/fullsize/ER-Stress---UPR-Antibody-Pack-Immunohistochemistry-NBP2-52746-img0008.jpg)
![Immunohistochemistry: ER Stress / UPR Antibody Pack [NBP2-52746] - P-Ire1a activation correlates with WFS1 cell expression. Cryosections of retina from 12 month old Wfs1+/+ and Wfs1-/- mouse were immunostained with anti-P-Ire1a antibody (red). DAPI was used for staining of cell nuclei (blue). RPE, retinal pigment epithelium; ONL, outer nuclear layer; INL, inner nuclear layer, RGC, retinal ganglion cells. Scale bars=50 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0097222) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ER-Stress---UPR-Antibody-Pack-Immunohistochemistry-NBP2-52746-img0009.jpg)
![Western Blot: ER Stress / UPR Antibody Pack [NBP2-52746] - NASTRp induces ER stress and eventually leads to cell death with Bim up-regulation. NASTRp induced ER stress and activated UPR led to apoptosis with Bim induction in NSCLC cell lines. Cells were treated with the indicated concentrations of NASTRp for 24 hours and cell lysates were subjected to SDS-PAGE/Western blot analysis using the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0122628) licensed under a CC-BY license. GADD153/CHOP Antibody [NBP2-13172]](http://images.novusbio.com/fullsize/ER-Stress---UPR-Antibody-Pack-Western-Blot-NBP2-52746-img0011.jpg)
![Western Blot: ER Stress / UPR Antibody Pack [NBP2-52746] - WB detection of total IRE1 alpha protein in lysates from Min6 cells which were transfected with GFP-siRNA or Ire1-siRNA.](http://images.novusbio.com/fullsize/ER-Stress---UPR-Antibody-Pack-Western-Blot-NBP2-52746-img0002.jpg)
![Western Blot: ER Stress / UPR Antibody Pack [NBP2-52746] - WB analysis of 293 cells transfected with full-length ATF6 (Lane 1), or with partial length amino acids 1-373 of ATF6 (Lane 2), and the non-transfected control cells (Lane 3). The ATF6* band is possibly an under-glycosylated or cleaved/active ATF6.](http://images.novusbio.com/fullsize/ER-Stress---UPR-Antibody-Pack-Western-Blot-NBP2-52746-img0006.jpg)
![Western Blot: ER Stress / UPR Antibody Pack [NBP2-52746] - Total protein from HeLa cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-XBP1 ([NBP1-77681) in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Arrow delineates XBP1s and arrowhead XBP1u.](http://images.novusbio.com/fullsize/ER-Stress---UPR-Antibody-Pack-Western-Blot-NBP2-52746-img0010.jpg)
![Immunohistochemistry-Paraffin: ER Stress / UPR Antibody Pack [NBP2-52746] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse bladder using XBP1 antibody ([NBP1-77681) with HRP-DAB detection and hematoxylin counterstaining.](http://images.novusbio.com/fullsize/ER-Stress---UPR-Antibody-Pack-Immunohistochemistry-Paraffin-NBP2-52746-img0005.jpg)
![Simple Western: ER Stress / UPR Antibody Pack [NBP2-52746] - Simple Western lane view shows a specific band for GRP78/HSPA5 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/ER-Stress---UPR-Antibody-Pack-Simple-Western-NBP2-52746-img0004.jpg)
![Flow Cytometry: TLR (Cell Surface) Screening Antibody Pack [NBP2-25086] - TLR1 and TLR2 expression pattern in primary BMNCs from 3 MM patients analyzed by flow cytometry. CD138-positive cells were gated from the total cell population. Staining for was compared with isotype-matched controls (NC). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license. TLR1 Antibody [NB100-56563] and TLR2 Antibody (TL2.1) [NB100-56722] .](http://images.novusbio.com/fullsize/TLR-Cell-Surface-Screening-Antibody-Pack-Flow-Cytometry-NBP2-25086-img0007.jpg "Flow Cytometry: TLR (Cell Surface) Screening Antibody Pack [NBP2-25086] - TLR1 and TLR2 expression pattern in primary BMNCs from 3 MM patients analyzed by flow cytometry. CD138-positive cells were gated from the total cell population. Staining for was compared with isotype-matched controls (NC). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license. TLR1 Antibody [NB100-56563] and TLR2 Antibody (TL2.1) [NB100-56722] .")
![<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: TLR (Cell Surface) Screening Antibody Pack [NBP2-25086] - Formalin-fixed paraffin-embedded tissue sections of human tonsil were probed for TLR4 mRNA (ACD RNAScope Probe, catalog #311281; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Mouse Monoclonal (Novus Biologicals catalog #NB100-56566) at 5ug/mL with 1 hour incubation at room temperature followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes.](http://images.novusbio.com/fullsize/TLR-Cell-Surface-Screening-Antibody-Pack-Dual-RNAscope-ISH-IHC-NBP2-25086-img0008.jpg)
![Western Blot: TLR (Cell Surface) Screening Antibody Pack [NBP2-25086] - Expression of TLR2 was determined by western blotting. The immunoreactivity of the anti-TLR2 was confirmed with human intestinal lysate. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license. TLR2 Antibody (TL2.1) [NB100-56722]](http://images.novusbio.com/fullsize/TLR-Cell-Surface-Screening-Antibody-Pack-Western-Blot-NBP2-25086-img0006.jpg)
![Immunohistochemistry-Paraffin: TLR (Cell Surface) Screening Antibody Pack [NBP2-25086] - Analysis of FFPE tissue section of normal human skin using 5 ug/ml concentration of TLR5 antibody (clone 19D759.2). Intense membrane staining of TLR5 was observed specifically in the pigmented basel cells of epidermal layer [10X Magnification]. TLR5 Antibody (19D759.2) [NBP2-24787]](http://images.novusbio.com/fullsize/TLR-Cell-Surface-Screening-Antibody-Pack-Immunohistochemistry-Paraffin-NBP2-25086-img0010.jpg)
![Flow Cytometry: TLR (Cell Surface) Screening Antibody Pack [NBP2-25086] - Detection of TLR10 on human PBMC using 10 ul (1 ug) of TLR10 antibody and 1 ug of CD19 antibody. Analysis using the Alexa Fluor (R) 647 conjugate of TLR10 antibody [NBP1-70343].](http://images.novusbio.com/fullsize/TLR-Cell-Surface-Screening-Antibody-Pack-Flow-Cytometry-NBP2-25086-img0011.jpg)
![Immunohistochemistry: TLR (Cell Surface) Screening Antibody Pack [NBP2-25086] - Pericryptal Myofibroblasts are Responsible for Increased TLR4 Expression in a Subset of CRCs. Double-stained immunofluorescence for TLR4 (green) and vimentin (red) in normal (I), adenoma (II), and colon adenocarcinoma (III) (10x). In the stromal compartment of CRCs, immunofluorescent staining for TLR4 localized to the pericryptal myofibroblasts in a subset of samples. Image collected and cropped by CiteAb from the following publication (//www.jeccr.com/content/33/1/45), licensed under a CC-BY license. TLR4 Antibody (76B357.1) [NB100-56566]](http://images.novusbio.com/fullsize/TLR-Cell-Surface-Screening-Antibody-Pack-Immunohistochemistry-NBP2-25086-img0009.jpg)
![Live Imaging Microscopy: Human ESC Marker Antibody Pack [NBP1-42825] - Pluripotent Li-iPSCs colonies derived from reprogramming of PLCs. iPSC colonies (encircled by dashed line) were positive for the surface pluripotency marker TRA-1-60 (red) during live staining. TRA-1-60 staining (red) is shown before (C) and afteroverlaying onto the corresponding phase contrast image (D). Scale bars = 250um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0221762) licensed under a CC-BY license. TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730]](http://images.novusbio.com/fullsize/Human-ESC-Marker-Antibody-Pack-Live-Imaging--Microscopy-NBP1-42825-img0011.jpg "Live Imaging Microscopy: Human ESC Marker Antibody Pack [NBP1-42825] - Pluripotent Li-iPSCs colonies derived from reprogramming of PLCs. iPSC colonies (encircled by dashed line) were positive for the surface pluripotency marker TRA-1-60 (red) during live staining. TRA-1-60 staining (red) is shown before (C) and afteroverlaying onto the corresponding phase contrast image (D). Scale bars = 250um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0221762) licensed under a CC-BY license. TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730]")
![Immunocytochemistry/Immunofluorescence: Human ESC Marker Antibody Pack [NBP1-42825] - TRA-1-81 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TRA-1-81 Monoclonal Antibody (Catalog # NB100-1833) at 10 ug/mL for 3 hours at room temperature. Cells were stained using an Anti-Mouse IgM Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. TRA-1-81 Antibody (TRA-1-81) [NB100-1833]](http://images.novusbio.com/fullsize/Human-ESC-Marker-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NBP1-42825-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Human ESC Marker Antibody Pack [NBP1-42825] - Analysis of human embryonic stem cells stained with SSEA-3 antibody detected with Alexa Fluor 488 anti-rat IgM secondary antibody. SSEA-3 Antibody (MC-631) [NB100-1832]](http://images.novusbio.com/fullsize/Human-ESC-Marker-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NBP1-42825-img0013.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Human ESC Marker Antibody Pack [NBP1-42825] - Detection of Mouse Nanog on HeLa whole cell lysate using NB100-588. Nanog was also IPed using NB100-587 and another Nanog. IP reactions were controlled using normal rabbit IgG. IPed Nanog was blotted using NB100-587.](http://images.novusbio.com/fullsize/Human-ESC-Marker-Antibody-Pack-Immunoprecipitation-NBP1-42825-img0009.jpg)
![Western Blot: Human ESC Marker Antibody Pack [NBP1-42825] - Whole cell protein from human ES cells (lane 1), human NTERA-2 cells (lane 2), mouse embryonic fibroblasts (MEF, lane 3) and mouse V6.5 ES cells (lane 4, NBP1-97433) were separated on a 7.5% gel and transferred to PVDF membrane. The membrane was then incubated with anti-TRA1-60(R) antibody at 2 ug/ml and detected with an anti-mouse IgM secondary antibody using chemiluminescence. Note how this antibody specifically detects the TRA1-60(R) epitope only on human cells. TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730]](http://images.novusbio.com/fullsize/Human-ESC-Marker-Antibody-Pack-Western-Blot-NBP1-42825-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Human ESC Marker Antibody Pack [NBP1-42825] - SSEA1 antibody was tested in Ntera-2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red). SSEA-1 Antibody (MC-480) [NB100-1831]](http://images.novusbio.com/fullsize/Human-ESC-Marker-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NBP1-42825-img0014.jpg)
![Flow Cytometry: Human ESC Marker Antibody Pack [NBP1-42825] - FACS analysis of Nanog expression in primary mouse hepatic stellate cell cultured for 7 days. Image from verified customer review.](http://images.novusbio.com/fullsize/Human-ESC-Marker-Antibody-Pack-Flow-Cytometry-NBP1-42825-img0015.jpg)
![Western Blot: Human ESC Marker Antibody Pack [NBP1-42825] - Detection of OCT4 in mouse brain lysate](http://images.novusbio.com/fullsize/Human-ESC-Marker-Antibody-Pack-Western-Blot-NBP1-42825-img0005.jpg)
![Flow Cytometry: Human ESC Marker Antibody Pack [NBP1-42825] - FACS staining of NTERA-2 cells using NB100-1831 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-mouse IgM secondary antibody.](http://images.novusbio.com/fullsize/Human-ESC-Marker-Antibody-Pack-Flow-Cytometry-NBP1-42825-img0003.jpg)
![Flow Cytometry: Human ESC Marker Antibody Pack [NBP1-42825] - FACS staining of NTERA-2 cells using NB100-2379 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit secondary antibody.](http://images.novusbio.com/fullsize/Human-ESC-Marker-Antibody-Pack-Flow-Cytometry-NBP1-42825-img0004.jpg)
![Western Blot: LC3 Antibody Pack [NBP2-59703] - LC3B was detected in immersion fixed Chloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B monoclonal antibody (1251A) [Catalog #NBP2-46892] at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NBP2-59703-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: LC3 Antibody Pack [NBP2-59703] - LC3B was detected in immersion fixed Chloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B monoclonal antibody (1251A) [Catalog #NBP2-46892] at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.")
![<b>Genetic Strategies Validation. </b>Western Blot: LC3 Antibody Pack [NBP2-59703] - LC3B was detected in immersion fixed Cloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B polyclonal antibody (Catalog #NB100-2220) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NBP2-59703-img0002.jpg)
![Immunohistochemistry: LC3 Antibody Pack [NBP2-59703] - IHC (Immunohistochemical) analysis of a formalin fixed and paraffin embedded tissue section of normal mouse brain using rabbit monoclonal LC3B (1251A) antibody [Catalog # NBP2-46892] at 1:100 dilution with HRP-DAB detection. The antibody generated a weak diffused cytoplasmic staining in most of the cells but some cells, especially within empty areas on the section, showed punctate signal also which signifies the presence of autophagy in those areas.](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NBP2-59703-img0005.jpg)
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: LC3 Antibody Pack [NBP2-59703] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NBP2-59703-img0001.jpg)
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: LC3 Antibody Pack [NBP2-59703] - Western Blot analysis shows lysates of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 2.0 ug/mL of Rabbit Anti-Human/Mouse/Rat LC3B Monoclonal Antibody (1251A) [Catalog # NBP2-46892] followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NBP2-59703-img0003.jpg)
![Western Blot: IRE1 alpha Antibody Pack [NBP2-50067] - Western Blot: IRE1 alpha [p Ser724] Antibody [NB100-2323] - Western blot of anti-IRE1 alpha (pSer724) using Lot AN and AO of NB100-2323. HeLa cells were treated (+) or untreated (-) with 10 mM DTT for 60 min to activate the UPR. Total protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% BSA in TBST. The membrane was probed with 2.0 ug/ml antibody in 5% BSA, and detected with an anti-rabbit HRP secondary antibody using chemiluminecence. Theoretical molecular weight: 110 kDa.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Pack-Western-Blot-NBP2-50067-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: IRE1 alpha Antibody Pack [NBP2-50067] - Western Blot: IRE1 alpha [p Ser724] Antibody [NB100-2323] - Western blot of anti-IRE1 alpha (pSer724) using Lot AN and AO of NB100-2323. HeLa cells were treated (+) or untreated (-) with 10 mM DTT for 60 min to activate the UPR. Total protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% BSA in TBST. The membrane was probed with 2.0 ug/ml antibody in 5% BSA, and detected with an anti-rabbit HRP secondary antibody using chemiluminecence. Theoretical molecular weight: 110 kDa.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: IRE1 alpha Antibody Pack [NBP2-50067] - P-Ire1a activation correlates with WFS1 cell expression. Cryosections of retina from 12 month old Wfs1+/+ and Wfs1-/- mouse were immunostained with anti-P-Ire1a antibody (red). DAPI was used for staining of cell nuclei (blue). RPE, retinal pigment epithelium; ONL, outer nuclear layer; INL, inner nuclear layer, RGC, retinal ganglion cells. Scale bars=50 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0097222) licensed under a CC-BY license. IRE1 alpha [p Ser724] Antibody [NB100-2323]](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Pack-Immunohistochemistry-NBP2-50067-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: IRE1 alpha Antibody Pack [NBP2-50067] - Western Blot detection of Total IRE1 Alpha in transfected Min6 cells using IRE1 alpha Antibody [NB100-2324]](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Pack-Western-Blot-NBP2-50067-img0002.jpg)
![Immunohistochemistry: IRE1 alpha Antibody Pack [NBP2-50067] - XBP1 and TG2 deficiency promote increased IRE1a protein levels in cultured VSMCs and in neointima in situ. (D) Xbp1WT and Xbp1 delta WT and (E) Tgm2 WT and Tgm2 delta carotid artery sections were analyzed by IHC for total IRE1a. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0212235) licensed under a CC-BY license. IRE1 alpha Antibody [NB100-2324]](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Pack-Immunohistochemistry-NBP2-50067-img0008.jpg)
![Immunohistochemistry-Frozen: LRRK2 Antibody Pack [NB100-938] - Staining of muscle sections as described in PMID 24312256. Image from verified customer review. LRRK2 Antibody [NB300-268]](http://images.novusbio.com/fullsize/LRRK2-Antibody-Pack-Immunohistochemistry-Frozen-NB100-938-img0017.jpg "Immunohistochemistry-Frozen: LRRK2 Antibody Pack [NB100-938] - Staining of muscle sections as described in PMID 24312256. Image from verified customer review. LRRK2 Antibody [NB300-268]")
![Western Blot: LRRK2 Antibody Pack [NB100-938] - LRRK2 N-terminal sequences show increased aggregation. Western blots of cell lysates of SH-SY5Y cells transfected with indicated LRRK2 construct showed equal expression of the LRRK2 protein with anti-HA (top panel) or anti-C-terminal- (bottom panel) LRRK2 antibody. Equal lysate loading is indicated by Beta-actin loading control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0045149) licensed under a CC-BY license. LRRK2 Antibody [NB300-268]](http://images.novusbio.com/fullsize/LRRK2-Antibody-Pack-Western-Blot-NB100-938-img0016.jpg)
![Immunohistochemistry: LRRK2 Antibody Pack [NB100-938] - Staining of neurons and glia using NB110-55289 at 2.5ug/ml. Mouse brain, 40x.](http://images.novusbio.com/fullsize/LRRK2-Antibody-Pack-Immunohistochemistry-NB100-938-img0013.jpg)
![Western Blot: LRRK2 Antibody Pack [NB100-938] - Detection of murine LRRK2 in mouse brain lysate using NB110-55289.](http://images.novusbio.com/fullsize/LRRK2-Antibody-Pack-Western-Blot-NB100-938-img0014.jpg)
![Western Blot: Neural Stem Cell Marker Antibody Pack [NBP1-42826] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and HMGB1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1.0 ug/ml of Rabbit Anti-Human HMGB1 Polyclonal Antibody (Catalog # NB100-2322) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HMGB1 at approximately 30 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Neural-Stem-Cell-Marker-Antibody-Pack-Knockout-Validated-NBP1-42826-img0018.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Neural Stem Cell Marker Antibody Pack [NBP1-42826] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and HMGB1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1.0 ug/ml of Rabbit Anti-Human HMGB1 Polyclonal Antibody (Catalog # NB100-2322) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HMGB1 at approximately 30 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry Free-Floating: Neural Stem Cell Marker Antibody Pack [NBP1-42826] - Analysis of rat cerebellum section stained with mouse GFAP mAb (NBP1-05197), dilution 1:1,000 (Green), costained with rabbit neurofilament NF-L pAb, dilution 1:2,000 (Red). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with antibodies. The GFAP antibody stains a network of astroglial cells, while the NF-L antibody labels neuronal cells and their processes.](http://images.novusbio.com/fullsize/Neural-Stem-Cell-Marker-Antibody-Pack-Immunohistochemistry-Free-Floating-NBP1-42826-img0019.jpg)
![Simple Western: Neural Stem Cell Marker Antibody Pack [NBP1-42826] - Simple Western lane view shows lysates of rat cortical neurons, loaded at 0.2 mg/mL. A specific band was detected for beta -III Tubulin at approximately 56 kDa (as indicated) using 10 ug/mL of Mouse Anti-Neuron-specific beta -III Tubulin Monoclonal Antibody (Catalog # MAB1195). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Neural-Stem-Cell-Marker-Antibody-Pack-Simple-Western-NBP1-42826-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Neural Stem Cell Marker Antibody Pack [NBP1-42826] - beta -III Tubulin in Differentiated Human Neural Progenitor Cells. beta -III Tubulin was detected in immersion fixed differentiated human neural progenitor cells using Neuron-specific beta -III Tubulin Monoclonal Antibody (clone TuJ-1) (Catalog # MAB1195) for 3 hours at room temperature. Cells were stained (green) and counterstained (red). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.](http://images.novusbio.com/fullsize/Neural-Stem-Cell-Marker-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NBP1-42826-img0013.jpg)
![Western Blot: Neural Stem Cell Marker Antibody Pack [NBP1-42826] - Detection of SOX2 in mouse brain lysate using NB110-37235 (0.5ug/ml).](http://images.novusbio.com/fullsize/Neural-Stem-Cell-Marker-Antibody-Pack-Western-Blot-NBP1-42826-img0003.jpg)
![Western Blot: Neural Stem Cell Marker Antibody Pack [NBP1-42826] - Western blot shows lysates of human brain (cerebellum) tissue, human brain (hypothalamus) tissue, mouse brain (cerebellum) tissue, and mouse brain (stem) tissue. PVDF membrane was probed with 0.2 ug/mL of Mouse Anti-Neuron-specific beta -III Tubulin Monoclonal Antibody (Catalog # MAB1195) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for beta -III Tubulin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.](http://images.novusbio.com/fullsize/Neural-Stem-Cell-Marker-Antibody-Pack-Western-Blot-NBP1-42826-img0023.jpg)
![Simple Western: Neural Stem Cell Marker Antibody Pack [NBP1-42826] - Simple Western lane view shows a specific band for GFAP in 0.05 mg/ml of Human Brain lysate using NBP1-05197. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Neural-Stem-Cell-Marker-Antibody-Pack-Simple-Western-NBP1-42826-img0020.jpg)
![Neural Stem Cell Marker Antibody Pack [NBP1-42826] - Total protein from SHSY-5Y, MCF7, Neuro2A and HeLa was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-HMGB1 [NB100-2322] in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Neural-Stem-Cell-Marker-Antibody-Pack-NBP1-42826-img0022.jpg)
![Immunohistochemistry: TLR (Intracellular) Screening Antibody Pack [NBP2-25085] - The expression of TLR-7 in the hippocampal brain region. The immunofluorescence of TLR7 recognized by Alexa 488, green. GFAP recognized by Alexa 594, red. NEUN recognized by Alexa 633, (blue) and merged image in the hippocampal region. NeuN and GFAP were applied to show the distribution of TLR7 within neuronal and supportive tissue populations. Scale bar 80 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0222818) licensed under a CC-BY license. TLR7 Antibody [NBP2-24906]](http://images.novusbio.com/fullsize/TLR-Intracellular-Screening-Antibody-Pack-Immunohistochemistry-NBP2-25085-img0007.jpg "Immunohistochemistry: TLR (Intracellular) Screening Antibody Pack [NBP2-25085] - The expression of TLR-7 in the hippocampal brain region. The immunofluorescence of TLR7 recognized by Alexa 488, green. GFAP recognized by Alexa 594, red. NEUN recognized by Alexa 633, (blue) and merged image in the hippocampal region. NeuN and GFAP were applied to show the distribution of TLR7 within neuronal and supportive tissue populations. Scale bar 80 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0222818) licensed under a CC-BY license. TLR7 Antibody [NBP2-24906]")
![Flow Cytometry: TLR (Intracellular) Screening Antibody Pack [NBP2-25085] - Expression of TLR3 and TLR8 in Fravel, L363, OPM1, RPMI8226, XG1, and NCI-H929 as determined by flow cytometry. HCMLs were stained using an intracellular staining protocol with TLR3 and TLR8 antibodies followed by relevant secondary fluorescent-conjugated antibodies. Filled histograms (purple) represent the isotype controls and the open histograms (red) indicate TLR8. Data are representative for analysis of >= 2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license. TLR3 Antibody (40C1285.6) [NBP2-24875] and TLR8 Antibody (44C143) [NBP2-24917]](http://images.novusbio.com/fullsize/TLR-Intracellular-Screening-Antibody-Pack-Flow-Cytometry-NBP2-25085-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TLR (Intracellular) Screening Antibody Pack [NBP2-25085] - Expression of TLR-9 protein in HB cells before and after 48 hr of transfection. Beta-actin was used as an internal control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092748) licensed under a CC-BY license. TLR9 Antibody (26C593.2) [NBP2-24729]](http://images.novusbio.com/fullsize/TLR-Intracellular-Screening-Antibody-Pack-Knockdown-Validated-NBP2-25085-img0010.jpg)
![Western Blot: TLR (Intracellular) Screening Antibody Pack [NBP2-25085] - All the cell lines displayed a strong expression of TLR3 and TLR8 proteins. To confirm the immunoreactivity of the antibody, different positive controls were included. Beta-actin was served as loading control and was used to normalize expression levels between cells. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR-Intracellular-Screening-Antibody-Pack-Western-Blot-NBP2-25085-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: TLR (Intracellular) Screening Antibody Pack [NBP2-25085] - Cbl-b is required for the endocytic transit of TLR9. Representative confocal microscopic images of splenocytes from mice with indicated genotypes. For experiments, cells were stimulated through the BCR (green) for 30 minutes then fixed and stained for TLR9 (blue) and Lamp-1 (red)(n = 3). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0089792) licensed under a CC-BY license. TLR9 Antibody (26C593.2) [NBP2-24729]](http://images.novusbio.com/fullsize/TLR-Intracellular-Screening-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NBP2-25085-img0011.jpg)
![Simple Western: TLR (Intracellular) Screening Antibody Pack [NBP2-25085] - Lane view shows a specific band for TLR9 in 0.5 mg/ml of Ramos lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system. Image using the Azide Free format of this antibody. TLR9 Antibody (26C593.2) [NBP2-24729]](http://images.novusbio.com/fullsize/TLR-Intracellular-Screening-Antibody-Pack-Simple-Western-NBP2-25085-img0009.jpg)