ER Stress / UPR Antibody Pack

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Western Blot: ER Stress / UPR Antibody Pack [NBP2-52746] - Hepatic ER stress markers with cachexia progression. ER stress markers Bip, IRE1a, ATF6p50 and CHOP were examined in the liver of non, pre and severely ...read more
Immunohistochemistry: ER Stress / UPR Antibody Pack [NBP2-52746] - XBP1 and TG2 deficiency promote increased IRE1a protein levels in cultured VSMCs and in neointima in situ. (D) Xbp1WT and Xbp1 delta WT and (E) Tgm2 WT ...read more
Immunohistochemistry: ER Stress / UPR Antibody Pack [NBP2-52746] - P-Ire1a activation correlates with WFS1 cell expression. Cryosections of retina from 12 month old Wfs1+/+ and Wfs1-/- mouse were immunostained with ...read more
Western Blot: ER Stress / UPR Antibody Pack [NBP2-52746] - NASTRp induces ER stress and eventually leads to cell death with Bim up-regulation. NASTRp induced ER stress and activated UPR led to apoptosis with Bim ...read more
Western Blot: ER Stress / UPR Antibody Pack [NBP2-52746] - WB detection of total IRE1 alpha protein in lysates from Min6 cells which were transfected with GFP-siRNA or Ire1-siRNA.
Western Blot: ER Stress / UPR Antibody Pack [NBP2-52746] - WB analysis of 293 cells transfected with full-length ATF6 (Lane 1), or with partial length amino acids 1-373 of ATF6 (Lane 2), and the non-transfected control ...read more
Western Blot: ER Stress / UPR Antibody Pack [NBP2-52746] - Total protein from HeLa cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was ...read more
Immunohistochemistry-Paraffin: ER Stress / UPR Antibody Pack [NBP2-52746] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse bladder using XBP1 antibody ([NBP1-77681) with HRP-DAB detection ...read more
Simple Western: ER Stress / UPR Antibody Pack [NBP2-52746] - Simple Western lane view shows a specific band for GRP78/HSPA5 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Applications WB, IHC, IHC-P

Order Details

ER Stress / UPR Antibody Pack Summary

Description
This UPR / ER Stress Sampler Kit is an antibody pack containing sample size vials of the top markers namely: GRP78/HSPA5, pSer724 IRE1 alpha, total IRE1 alpha, XBP1, ATF6, and CHOP/GADD153.
Immunogen
See individual datasheets.

Applications/Dilutions

Application Notes
Applications are antibody dependent. See individual datasheets for validated applications.

Reactivity Notes

Species reactivity is antibody dependent. Please refer to individual datasheets for more specific information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Kit Components

Notes

This pack contains 1 vial each of: NBP1-06277SS (0.025 mL), NB100-2323SS (0.025 mL), NB100-2324SS (0.025 mL), NBP1-77681SS (0.025 mL), NBP1-40256SS (0.025 mg), NBP2-13172-0.025ml (0.025 mL).

Alternate Names for ER Stress / UPR Antibody Pack

  • anti-Growth arrest and DNA damage-inducible protein GADD153 antibody
  • ire1 alpha p Ser724
  • phospho ire1 alpha
  • phospho-ire1 alpha
  • p-ire1 alpha

Background

Unfolded protein response (UPR) signaling is a mechanism used by eukaryotic cells to deal with endoplasmic reticulum/ER stress: an accumulation of unfolded proteins in the ER lumen. It can be triggered by conditions such as high protein demand, viral infection, mutant protein expression, hypoxia, energy deprivation, or exposure to excessive oxidative stress. UPR executes three major functions: (i) blocking of protein translation for restoring homeostasis; (ii) positive regulation of protein folding related molecular chaperones; and (iii) up-regulation of signaling responsible for targeting mis-/un-folded proteins in ER for ubiquitination mediated degradation. Besides promoting survival, UPR can induce apoptosis and cause cell death, especially under ER-stress. A sustained over-activation of UPR is known to be involved in many human diseases including cancer, hyperglycemia, autoimmune conditions, hepatic disorders, retinopathies, acute lung injury and neuro-degeneration. This is why, a complete mechanistic understanding of UPR signaling is very critical for the evaluation of its biological effects in normal and/or disease conditions. UPR is regulated via three major effector proteins, namely inositol-requiring enzyme 1 (IRE1), activating transcription factor 6 (ATF6) and protein kinase RNA-like ER kinase (PERK), which are maintained in their inactive/membrane bound state through binding with glucose response protein 78 (GRP78/BiP). In UPR/ER stress experiments, the expression of major marker proteins such as IRE1-alpha, XBP1, GPR78, ATF6, and CHOP are often analyzed using various immunoassays. Modulation of individual marker proteins can provide an understanding of which signaling arm (i.e. IRE1 alpha, PERK, ATF6) of the UPR is being inhibited or activated. For example, an increase in phospho-IRE1 alpha (Ser724) and/or XBP1-S levels indicates the activation of IRE1 alpha mediated signaling. CHOP induction is an indication of ATF6 and PERK signaling activation, while the levels of GRP78 and ERp72 are more specific indicators of ATF6 activation. ER Stress/UPR Antibody Pack contains sample sizes of six antibodies against major UPR signaling markers - GRP78, pIRE1 alpha, total IRE1 alpha, XBP1, ATF6, and CHOP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

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Video Protocols

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