TLR (Intracellular) Screening Antibody Pack

Immunohistochemistry: TLR (Intracellular) Screening Antibody Pack [NBP2-25085] - The expression of TLR-7 in the hippocampal brain region. The immunofluorescence of TLR7 recognized by Alexa 488, green. GFAP recognized by Alexa 594, red. NEUN recognized by Alexa 633, (blue) and merged image in the hippocampal region. NeuN and GFAP were applied to show the distribution of TLR7 within neuronal and supportive tissue populations. Scale bar 80 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0222818) licensed under a CC-BY license. TLR7 Antibody [NBP2-24906]

Flow Cytometry: TLR (Intracellular) Screening Antibody Pack [NBP2-25085] - Expression of TLR3 and TLR8 in Fravel, L363, OPM1, RPMI8226, XG1, and NCI-H929 as determined by flow cytometry. HCMLs were stained using an intracellular staining protocol with TLR3 and TLR8 antibodies followed by relevant secondary fluorescent-conjugated antibodies. Filled histograms (purple) represent the isotype controls and the open histograms (red) indicate TLR8. Data are representative for analysis of >= 2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license. TLR3 Antibody (40C1285.6) [NBP2-24875] and TLR8 Antibody (44C143) [NBP2-24917]

Western Blot: TLR (Intracellular) Screening Antibody Pack [NBP2-25085] - Expression of TLR-9 protein in HB cells before and after 48 hr of transfection. Beta-actin was used as an internal control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092748) licensed under a CC-BY license. TLR9 Antibody (26C593.2) [NBP2-24729]

Western Blot: TLR (Intracellular) Screening Antibody Pack [NBP2-25085] - All the cell lines displayed a strong expression of TLR3 and TLR8 proteins. To confirm the immunoreactivity of the antibody, different positive controls were included. Beta-actin was served as loading control and was used to normalize expression levels between cells. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: TLR (Intracellular) Screening Antibody Pack [NBP2-25085] - Cbl-b is required for the endocytic transit of TLR9. Representative confocal microscopic images of splenocytes from mice with indicated genotypes. For experiments, cells were stimulated through the BCR (green) for 30 minutes then fixed and stained for TLR9 (blue) and Lamp-1 (red)(n = 3). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0089792) licensed under a CC-BY license. TLR9 Antibody (26C593.2) [NBP2-24729]

Simple Western: TLR (Intracellular) Screening Antibody Pack [NBP2-25085] - Lane view shows a specific band for TLR9 in 0.5 mg/ml of Ramos lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system. Image using the Azide Free format of this antibody. TLR9 Antibody (26C593.2) [NBP2-24729]

• Reactivity: Hu, Mu
• Applications: WB, DB, Flow, ICC/IF, IHC, IP

TLR (Intracellular) Screening Antibody Pack Summary

• Immunogen: See individual datasheets.

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: See individual datasheets of components for their validated applications

Reactivity Notes

See individual datasheets of components for their validated species

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background

The Toll-like receptor (TLR) family in mammals comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLR3, 7, 8, and 9 are expressed in the intracellular organelles. TLR7 and TLR8 have a common ligand, viral single-stranded RNA. TLR3 is activated by viral double-stranded RNA. TLR9 responds to bacterial unmethylated CpG DNA. Ten human homologs of TLRs (TLR1-10) have been described

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.