Vimentin Antibodies
Vimentin is a type III intermediate filament protein that plays an essential role in cytoskeletal function and is largely expressed by mesenchymal cells. Human vimentin monomer protein is 466 amino acids in length with theoretical molecular weight of ~54 kDa. We offer multiple 5-star rated antibodies to detect Vimentin that have been cited in 100+ publications. Browse our extensive catalog for recombinant monoclonal antibodies, monoclonal antibodies, polyclonal antibodies, and Vimentin-conjugated antibodies. Validated research applications for our Vimentin-antibodies include CyTOF-Ready, Dual RNAscope ISH-IHC, ELISA, Flow Cytometry, ICC, Immunofluorescence, IHC-Frozen, IHC-Paraffin, Immunoprecipitation, Protein Array, Simple Western, and Knockout Validated Western Blot. Our Vimentin antibodies are suitable for use in many species: Human, Mouse, Rat, Bovine, Chicken, Goat, Hamster, Porcine, Primate, and Rabbit, to name a few. Each Vimentin antibody is fully covered by Novus’ Guarantee+. Choose the anti-Vimentin antibody best suited for your research needs by viewing the technical details, images, references, reviews, and related products.
Alternate Names for Vimentin Antibodies
anti-Vimentin antibody, anti-VIM antibody, anti-FLJ36605 antibody
39 Results for "vimentin" in Primary Antibodies
![Knockout Validated: Vimentin Antibody (VM452) [NBP2-29433] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-29433) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Knockout-Validated-NBP2-29433-img0003.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Vimentin Antibody (VM452) [NBP2-29433] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-29433) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (VM452) [NBP2-29433] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Western-Blot-NBP2-29433-img0008.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM452) [NBP2-29433] - Formalin-paraffin human melanoma stained with Vimentin Ab (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Immunohistochemistry-Paraffin-NBP2-29433-img0002.jpg)
![Flow Cytometry: Vimentin Antibody (VM452) [NBP2-29433] - Flow Cytometry of human Vimentin on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Flow-Cytometry-NBP2-29433-img0004.jpg)
![Western Blot: Vimentin Antibody (VM452) [NBP2-29433] - Western Blot Analysis Raji cell lysate Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Western-Blot-NBP2-29433-img0007.jpg)
![Protein Array: Vimentin Antibody (VM452) [NBP2-29433] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Vimentin Antibody (VM452). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Protein-Array-NBP2-29433-img0006.jpg)
![Western Blot: Vimentin Antibody (VM1170) [NBP2-44833] - Western Blot Analysis of HEP2 cell lysate using Vimentin Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Western-Blot-NBP2-44833-img0008.jpg "Western Blot: Vimentin Antibody (VM1170) [NBP2-44833] - Western Blot Analysis of HEP2 cell lysate using Vimentin Antibody (VM1170).")
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Vimentin Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0007.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Angiosarcoma stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0001.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Ewing's Sarcoma stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0002.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Leiomyosarcoma stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0003.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Melanoma stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0004.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Rhabdomyosarcoma stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0005.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Uterus stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0006.jpg)
![Western Blot: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Western Blot Analysis of human U-87 cell lysate using Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Western-Blot-NBP3-07191-img0005.jpg "Western Blot: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Western Blot Analysis of human U-87 cell lysate using Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).")
![Immunohistochemistry-Paraffin: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Formalin-fixed, paraffin-embedded human tonsil stained with Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Immunohistochemistry-Paraffin-NBP3-07191-img0003.jpg)
![Western Blot: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Western Blot Analysis of human A375 cell lysate using Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Western-Blot-NBP3-07191-img0004.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Formalin-fixed, paraffin-embedded human melanoma stained with Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Immunohistochemistry-Paraffin-NBP3-07191-img0001.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Immunohistochemistry-Paraffin-NBP3-07191-img0002.jpg)
![Immunoprecipitation: Vimentin Antibody (SC60-05) [NBP3-33094] - Vimentin was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-33094 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33094 at 1/10,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-33094 in HeLa cell lysate Lane 3: NBP3-33094 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-2682024158265.jpg "Immunoprecipitation: Vimentin Antibody (SC60-05) [NBP3-33094] - Vimentin was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-33094 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33094 at 1/10,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-33094 in HeLa cell lysate Lane 3: NBP3-33094 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds")
![Immunohistochemistry-Frozen: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunofluorescence analysis of frozen mouse hippocampus tissue labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33094).The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33094, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-2682024159390.jpg)
![Immunohistochemistry: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33094) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-298202414161822.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunocytochemistry analysis of C6 cells labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-29202410234261.jpg)
![Flow Cytometry: Vimentin Antibody (SC60-05) [NBP3-33094] - Flow cytometric analysis of HeLa (positive, red) and MCF7 (negative, green) cells labeling Vimentin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33094) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (HeLa black, MCF7 light green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-29202416453228.jpg)
![Western Blot: Vimentin Antibody (SC60-05) [NBP3-33094] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/20,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: HEK-293 cell lysate (10 µg/Lane) Lane 3: Jurkat cell lysate (10 µg/Lane) Lane 4: C2C12 cell lysate (10 µg/Lane) Lane 5: RAW264.7 cell lysate (10 µg/Lane) Lane 6: C6 cell lysate (10 µg/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-3920248453527.jpg)
![Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-52867) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Knockout-Validated-NBP2-52867-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-52867) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Analysis in human cell lines U-251MG and MCF-7 using Anti-VIM antibody. Corresponding VIM RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Western-Blot-NBP2-52867-img0010.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human skin shows distinct positivity in fibroblasts.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Immunohistochemistry-Paraffin-NBP2-52867-img0005.jpg)
![Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Lane 1: Marker [kDa] Lane 2: Human tonsil tissue lysate](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Western-Blot-NBP2-52867-img0006.jpg)
![Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Lane 1: Marker [kDa], Lane 2: Human cell line HeLa cytoplasmic fraction , Lane 3: Human cell line HeLa membrane fraction , Lane 4: Human cell line HeLa nuclear fraction , Lane 5: Human cell line HeLa chromatin fraction , Lane 6: Human cell line HeLa cytos](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Western-Blot-NBP2-52867-img0007.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human cerebellum shows immunoreactivity in the endothelial cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Immunohistochemistry-Paraffin-NBP2-52867-img0001.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human kidney shows strong immunoreactivity in renal glomeruli but not tubuli cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Immunohistochemistry-Paraffin-NBP2-52867-img0002.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human prostate shows strong immunoreactivity in the connective tissue.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Immunohistochemistry-Paraffin-NBP2-52867-img0003.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human rectum shows strong positivity in mesenchymal and lymphoid cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Immunohistochemistry-Paraffin-NBP2-52867-img0004.jpg)
![Western Blot: Vimentin Antibody (SPM576) [NBP2-32910] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (SPM576).](http://images.novusbio.com/fullsize/Vimentin-Antibody-SPM576-Western-Blot-NBP2-32910-img0002.jpg "Western Blot: Vimentin Antibody (SPM576) [NBP2-32910] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (SPM576).")
![Immunohistochemistry-Paraffin: Vimentin Antibody (SPM576) [NBP2-32910] - Formalin-paraffin human melanoma stained with Vimentin MAb (SPM576).](http://images.novusbio.com/fullsize/Vimentin-Antibody-SPM576-Immunohistochemistry-Paraffin-NBP2-32910-img0001.jpg)
![Western Blot: Vimentin Antibody (2A52) [NBP1-92688] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 1:10,000 of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP1-92688) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2A52-Knockout-Validated-NBP1-92688-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (2A52) [NBP1-92688] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 1:10,000 of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP1-92688) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Vimentin Antibody (2A52) [NBP1-92688] - Immunocytochemistry/Immunofluorescence: [NBP1-92688] - View of mixed neuron/glial cultures stained with NBP1-92687 (green) and the GFAP rabbit polyclonal (NB300-141, red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the imate which appear green. Astrocytes may express primarily GFAP, or GFAP and Vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and Vimentin, the two proteins assemble to produce heteropolymer filaments.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2A52-Immunocytochemistry-Immunofluorescence-NBP1-92688-img0005.jpg)
![Western Blot: Vimentin Antibody (2A52) [NBP1-92688] - Analysis of cell and whole brain tissue lysates using mouse mAb to vimentin, NBP1-92688, dilution 1:5,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS-1, [6] C6, and [7] rat brain. The band at about 50kDa mark corresponds to vimentin protein. The antibody does not recognize mouse vimentin (not shown).](http://images.novusbio.com/fullsize/Vimentin-Antibody-2A52-Western-Blot-NBP1-92688-img0006.jpg)
![Western Blot: Vimentin Antibody (PDH0-01) [NBP3-33093] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/5,000 dilution. Lane 1: L6 cell lysate Lane 2: C2C12 cell lysate Lane 3: HeLa cell lysate Lane 4: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 54 kDa Observed band size: 57 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33093) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-288202414191539.jpg "Western Blot: Vimentin Antibody (PDH0-01) [NBP3-33093] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/5,000 dilution. Lane 1: L6 cell lysate Lane 2: C2C12 cell lysate Lane 3: HeLa cell lysate Lane 4: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 54 kDa Observed band size: 57 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33093) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Vimentin Antibody (PDH0-01) [NBP3-33093] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33093) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-29820241433367.jpg)
![Immunohistochemistry: Vimentin Antibody (PDH0-01) [NBP3-33093] - Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33093, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-298202414304144.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (PDH0-01) [NBP3-33093] - Immunocytochemistry analysis of HeLa cells labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-2920241114843.jpg)
![Flow Cytometry: Vimentin Antibody (PDH0-01) [NBP3-33093] - Flow cytometric analysis of Hela cells labeling Vimentin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33093, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-392024884612.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (979542) [Unconjugated] [MAB21053] - Vimentin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # MAB21053) at 8 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm (cytoskeleton).](http://images.novusbio.com/fullsize/Vimentin-Antibody-979542-Unconjugated-Immunocytochemistry-Immunofluorescence-MAB21053-img0001.jpg "Immunocytochemistry/Immunofluorescence: Vimentin Antibody (979542) [Unconjugated] [MAB21053] - Vimentin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # MAB21053) at 8 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm (cytoskeleton).")
![Immunohistochemistry-Paraffin: Vimentin Antibody (979542) [Unconjugated] [MAB21053] - Vimentin was detected in immersion fixed paraffin-embedded sections of human lymph node tissue using Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # MAB21053) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes in germinal centers.](http://images.novusbio.com/fullsize/Vimentin-Antibody-979542-Unconjugated-Immunohistochemistry-Paraffin-MAB21053-img0002.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (LN-6) [NBP2-44832] - Human Melanoma stained with Vimentin Monoclonal Antibody (LN-6).](http://images.novusbio.com/fullsize/Vimentin-Antibody-LN-6-Immunohistochemistry-Paraffin-NBP2-44832-img0002.jpg "Immunohistochemistry-Paraffin: Vimentin Antibody (LN-6) [NBP2-44832] - Human Melanoma stained with Vimentin Monoclonal Antibody (LN-6).")
![Western Blot: Vimentin Antibody (SPM576) - Azide and BSA Free [NBP2-34809] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (SPM576).](http://images.novusbio.com/fullsize/Vimentin-Antibody-SPM576-Azide-and-BSA-Free-Western-Blot-NBP2-34809-img0001.jpg "Western Blot: Vimentin Antibody (SPM576) - Azide and BSA Free [NBP2-34809] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (SPM576).")
![Immunohistochemistry-Paraffin: Vimentin Antibody (SPM576) - Azide and BSA Free [NBP2-34809] - Formalin-paraffin human melanoma stained with Vimentin MAb (SPM576).](http://images.novusbio.com/fullsize/Vimentin-Antibody-SPM576-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34809-img0002.jpg)
![Western Blot: Vimentin Antibody [NBP3-03221] - Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Vimentin antibody (NBP3-03221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Western-Blot-NBP3-03221-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody [NBP3-03221] - Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Vimentin antibody (NBP3-03221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Vimentin Antibody [NBP3-03221] - Immunohistochemistry of paraffin-embedded human colon using [KO Validated] Vimentin Rabbit pAb (NBP3-03221) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP3-03221-img0002.jpg)
![Western Blot: Vimentin Antibody [NBP3-03221] - Western blot analysis of extracts of various cell lines, using Vimentin antibody (NBP3-03221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Vimentin Antibody-Western Blot-NBP3-03221-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody - Azide and BSA Free [Vimentin] - Immunofluorescence analysis of U2OS cells using [KD Validated] Vimentin Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03221_rabbit-polyclonal-vimentin-antibody-5220251628096.jpg)