VCAM-1/CD106 Antibodies

210-TA) for 24 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human VCAM‑1/CD106 Monoclonal Antibody (Catalog # MAB8091) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for VCAM‑1/CD106 at approximately 110 kDa (as indicated). GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> 210-TA) for 24 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human VCAM‑1/CD106 Monoclonal Antibody (Catalog # MAB8091) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for VCAM‑1/CD106 at approximately 110 kDa (as indicated). GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 10 ng/mL Recombinant Human TNF‑ alpha (210-TA) for 24 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human VCAM‑1/CD106 Monoclonal Antibody (Catalog # MAB8091) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for VCAM‑1/CD106 at approximately 110 kDa (as indicated). GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_lightbox"> MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to splenocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to splenocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

210-TA) for 24 hrs, loaded at 0.2 mg/mL. A specific band was detected for VCAM‑1/CD106 at approximately 132 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human VCAM‑1/CD106 Monoclonal Antibody (Catalog # MAB8091) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox"> 210-TA) for 24 hrs, loaded at 0.2 mg/mL. A specific band was detected for VCAM‑1/CD106 at approximately 132 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human VCAM‑1/CD106 Monoclonal Antibody (Catalog # MAB8091) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

210-TA) for 24 hours. PVDF membrane was probed with 4 µg/mL of Rabbit Anti-Human VCAM‑1/CD106 Monoclonal Antibody (Catalog # MAB8092) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for VCAM‑1/CD106 at approximately 110 kDa (as indicated). GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> 210-TA) for 24 hours. PVDF membrane was probed with 4 µg/mL of Rabbit Anti-Human VCAM‑1/CD106 Monoclonal Antibody (Catalog # MAB8092) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for VCAM‑1/CD106 at approximately 110 kDa (as indicated). GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 10 ng/mL Recombinant Human TNF‑ alpha (210-TA) for 24 hours. PVDF membrane was probed with 4 µg/mL of Rabbit Anti-Human VCAM‑1/CD106 Monoclonal Antibody (Catalog # MAB8092) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for VCAM‑1/CD106 at approximately 110 kDa (as indicated). GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

MAB1050, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (F0110). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_lightbox"> MAB1050, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (F0110). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_thumb" />

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox"> DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." alt="VCAM1 was detected in immersion fixed paraffin-embedded sections of human Tonsil using Mouse Anti-Human VCAM1, Monoclonal Antibody (Catalog #MAB11671) at 15ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins." alt="HuT 78 human cutaneous T cell lymphoma cell line was stained with Mouse Anti-Human VCAM-1/CD106 PE-conjugated Monoclonal Antibody (Catalog # FAB5649P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />