TNF-alpha Antibodies

HAF017). Specific bands were detected for TNF-a at approximately 17 and 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). Specific bands were detected for TNF-a at approximately 17 and 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- alpha. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for TNF-a at approximately 17 and 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF017). Specific bands were detected for TNF-a at approximately 16 and 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). Specific bands were detected for TNF-a at approximately 16 and 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

410-MT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF-a (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Human/Mouse TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA). The ND₅₀ is typically 1.5-10 ng/mL in the presence of the metabolic inhibitor actinomycin D." class="big_lightbox"> 410-MT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF-a (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Human/Mouse TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA). The ND₅₀ is typically 1.5-10 ng/mL in the presence of the metabolic inhibitor actinomycin D." class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with saponin. View our protocol for Staining Intracellular Molecules." class="big_lightbox"> AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with saponin. View our protocol for Staining Intracellular Molecules." class="big_thumb" />

410-MT) was serially diluted and captured by Goat Anti-Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 410-MT) was serially diluted and captured by Goat Anti-Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

410-MT) was serially diluted and captured by Goat Anti-Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 410-MT) was serially diluted and captured by Goat Anti-Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

210-TA), Recombinant Mouse TNF-a aa 80-235 (Catalog # 410-MT), Recombinant Rat TNF-a (Catalog # 510-RT), and Recombinant Human APRIL/TNFSF13 (Catalog # 5860-AP). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human TNF-a Monoclonal Antibody (Catalog # MAB610) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TNF-a at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox"> 210-TA), Recombinant Mouse TNF-a aa 80-235 (Catalog # 410-MT), Recombinant Rat TNF-a (Catalog # 510-RT), and Recombinant Human APRIL/TNFSF13 (Catalog # 5860-AP). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human TNF-a Monoclonal Antibody (Catalog # MAB610) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TNF-a at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows 25 ng of Recombinant Human TNF-a (Catalog # 210-TA), Recombinant Mouse TNF-a aa 80-235 (Catalog # 410-MT), Recombinant Rat TNF-a (Catalog # 510-RT), and Recombinant Human APRIL/TNFSF13 (Catalog # 5860-AP). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human TNF-a Monoclonal Antibody (Catalog # MAB610) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TNF-a at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF-a (0.25 ng/mL) is neutral-ized (green line) by increasing concentrations of Mouse Anti-Human TNF-a Monoclonal Antibody (Catalog # MAB610). The ND₅₀ is typically 0.01-0.04 µg/mL in the presence of the metabolic inhibitor actinomycin D." class="big_lightbox"> 210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF-a (0.25 ng/mL) is neutral-ized (green line) by increasing concentrations of Mouse Anti-Human TNF-a Monoclonal Antibody (Catalog # MAB610). The ND₅₀ is typically 0.01-0.04 µg/mL in the presence of the metabolic inhibitor actinomycin D." class="big_thumb" />

DY990). Goat Anti-Human TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-210-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> DY990). Goat Anti-Human TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-210-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

510-RT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin. Cytotoxicity elicited by Recombinant Rat TNF-a (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-510-NA). The ND₅₀ is typically 0.15-0.75 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_lightbox"> 510-RT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin. Cytotoxicity elicited by Recombinant Rat TNF-a (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-510-NA). The ND₅₀ is typically 0.15-0.75 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." alt="Recombinant Rat TNF-a (Catalog # 510-RT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin. Cytotoxicity elicited by Recombinant Rat TNF-a (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-510-NA). The ND₅₀ is typically 0.15-0.75 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

510-RT) was serially diluted and captured by Mouse Anti-Rat TNF‑ alpha Monoclonal Antibody (Catalog # MAB510) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-510-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 510-RT) was serially diluted and captured by Mouse Anti-Rat TNF‑ alpha Monoclonal Antibody (Catalog # MAB510) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-510-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

210-TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF-a (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-210-NA). The ND₅₀ is typically 0.01-0.06 µg/mL." class="big_lightbox"> 210-TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF-a (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-210-NA). The ND₅₀ is typically 0.01-0.06 µg/mL." alt="Recombinant Human TNF-a (Catalog # 210-TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF-a (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-210-NA). The ND₅₀ is typically 0.01-0.06 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

MAB610) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-210-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> MAB610) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-210-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

MAB005, open histogram) followed by Anti-Rat IgG CFS-conjugated Secondary Antibody (F0104). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol. " class="big_lightbox"> MAB005, open histogram) followed by Anti-Rat IgG CFS-conjugated Secondary Antibody (F0104). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol. " alt="Raw264 mouse macrophage cell line treated with 1 μg/mL LPS overnight was stained with Rat Anti-Mouse TNF-alpha Monoclonal Antibody (Catalog # MAB4101, filled histogram) or isotype control antibody (MAB005, open histogram) followed by Anti-Rat IgG CFS-conjugated Secondary Antibody (F0104). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol. " class="big_thumb" />

F0105B) and Rat anti-Mouse CD3 APC-conjugated Monoclonal Antibody (FAB4841A). Quadrant markers were set based on isotype control antibody (MAB005). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our protocol for Staining Intracellular Molecules. " class="big_lightbox"> F0105B) and Rat anti-Mouse CD3 APC-conjugated Monoclonal Antibody (FAB4841A). Quadrant markers were set based on isotype control antibody (MAB005). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our protocol for Staining Intracellular Molecules. " class="big_thumb" />

410-MT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF-a (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse TNF-a Monoclonal Antibody (Catalog # MAB4101). The ND₅₀ is typically 0.15-0.75 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_lightbox"> 410-MT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF-a (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse TNF-a Monoclonal Antibody (Catalog # MAB4101). The ND₅₀ is typically 0.15-0.75 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_thumb" />

210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF-a (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF-a Monoclonal Antibody (Catalog # MAB210). The ND₅₀ is typically 0.01-0.06 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_lightbox"> 210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF-a (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF-a Monoclonal Antibody (Catalog # MAB210). The ND₅₀ is typically 0.01-0.06 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." alt="Recombinant Human TNF-a (Catalog # 210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF-a (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF-a Monoclonal Antibody (Catalog # MAB210). The ND₅₀ is typically 0.01-0.06 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_thumb" />

510-RT) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Rat TNF‑a (0.025 ng/mL) is neutralized (green line) by increasing concentrations of Rat TNF‑a Monoclonal Antibody (Catalog # MAB510). The ND₅₀ is typically 10-40 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_lightbox"> 510-RT) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Rat TNF‑a (0.025 ng/mL) is neutralized (green line) by increasing concentrations of Rat TNF‑a Monoclonal Antibody (Catalog # MAB510). The ND₅₀ is typically 10-40 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." alt="Recombinant Rat TNF‑a (Catalog # 510-RT) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Rat TNF‑a (0.025 ng/mL) is neutralized (green line) by increasing concentrations of Rat TNF‑a Monoclonal Antibody (Catalog # MAB510). The ND₅₀ is typically 10-40 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_thumb" />

510-RT) was serially diluted and captured by Mouse Anti-Rat TNF‑ alpha Monoclonal Antibody (Catalog # MAB510) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-510-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 510-RT) was serially diluted and captured by Mouse Anti-Rat TNF‑ alpha Monoclonal Antibody (Catalog # MAB510) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-510-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

410-MT) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Mouse TNF‑a (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse TNF‑a Polyclonal Antibody (Catalog # AB-410-NA). The ND₅₀ is typically 0.2-0.6 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_lightbox"> 410-MT) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Mouse TNF‑a (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse TNF‑a Polyclonal Antibody (Catalog # AB-410-NA). The ND₅₀ is typically 0.2-0.6 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." alt="Recombinant Mouse TNF‑a (Catalog # 410-MT) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Mouse TNF‑a (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse TNF‑a Polyclonal Antibody (Catalog # AB-410-NA). The ND₅₀ is typically 0.2-0.6 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_thumb" />

210-TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF-a (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human TNF-a Monoclonal Antibody (Catalog # MAB2101). The ND₅₀ is typically 0.015-0.06 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_lightbox"> 210-TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF-a (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human TNF-a Monoclonal Antibody (Catalog # MAB2101). The ND₅₀ is typically 0.015-0.06 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." alt="Recombinant Human TNF-a (Catalog # 210-TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF-a (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human TNF-a Monoclonal Antibody (Catalog # MAB2101). The ND₅₀ is typically 0.015-0.06 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_thumb" />

MAB002) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B) and Mouse anti-Human CD14 APC-conjugated Monoclonal Antibody (FAB3832A). Staining was perfomed using our Staining Membrane-associated Proteins protocol." class="big_lightbox"> MAB002) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B) and Mouse anti-Human CD14 APC-conjugated Monoclonal Antibody (FAB3832A). Staining was perfomed using our Staining Membrane-associated Proteins protocol." class="big_thumb" />