TNF-alpha Antibodies

210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF-a (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human TNF-a Polyclonal Antibody (Catalog # AB-210-NA). The ND₅₀ is typically 0.05-0.25 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_lightbox"> 210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF-a (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human TNF-a Polyclonal Antibody (Catalog # AB-210-NA). The ND₅₀ is typically 0.05-0.25 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." alt="Recombinant Human TNF-a (Catalog # 210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF-a (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human TNF-a Polyclonal Antibody (Catalog # AB-210-NA). The ND₅₀ is typically 0.05-0.25 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_thumb" />

410-MT), Recombinant Human TNF-a (Catalog # 210-TA), Recombinant Rat TNF-a (Catalog # 510-RT) and Recombinant Mouse Lymphotoxin- a/TNF- beta . PVDF Membrane was probed with 1 µg/mL of Hamster Anti-Mouse TNF-a Monoclonal Antibody (Catalog # MAB410) followed by HRP-conjugated Anti-Hamster IgG Secondary Antibody. A specific band was detected for TNF-a at approximately 15 kDa (as indicated). This experiment was conducted under reducing and non reducing conditions using Immunoblot Buffer Group 3." class="big_lightbox"> 410-MT), Recombinant Human TNF-a (Catalog # 210-TA), Recombinant Rat TNF-a (Catalog # 510-RT) and Recombinant Mouse Lymphotoxin- a/TNF- beta . PVDF Membrane was probed with 1 µg/mL of Hamster Anti-Mouse TNF-a Monoclonal Antibody (Catalog # MAB410) followed by HRP-conjugated Anti-Hamster IgG Secondary Antibody. A specific band was detected for TNF-a at approximately 15 kDa (as indicated). This experiment was conducted under reducing and non reducing conditions using Immunoblot Buffer Group 3." alt="Western blot shows 25 ng of Recombinant Mouse TNF-a aa 80-235 (Catalog # 410-MT), Recombinant Human TNF-a (Catalog # 210-TA), Recombinant Rat TNF-a (Catalog # 510-RT) and Recombinant Mouse Lymphotoxin- a/TNF- beta . PVDF Membrane was probed with 1 µg/mL of Hamster Anti-Mouse TNF-a Monoclonal Antibody (Catalog # MAB410) followed by HRP-conjugated Anti-Hamster IgG Secondary Antibody. A specific band was detected for TNF-a at approximately 15 kDa (as indicated). This experiment was conducted under reducing and non reducing conditions using Immunoblot Buffer Group 3." class="big_thumb" />

690-PT) induces cytotoxicity in the the PK-15 porcine kidney epithelial cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Porcine TNF-a (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF690). The ND₅₀ is typically 0.2-0.8 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_lightbox"> 690-PT) induces cytotoxicity in the the PK-15 porcine kidney epithelial cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Porcine TNF-a (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF690). The ND₅₀ is typically 0.2-0.8 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." alt="Recombinant Porcine TNF-a (Catalog # 690-PT) induces cytotoxicity in the the PK-15 porcine kidney epithelial cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Porcine TNF-a (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF690). The ND₅₀ is typically 0.2-0.8 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

690-PT) induces cytotoxicity in the the PK-15 porcine kidney epithelial cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Porcine TNF-a (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine TNF-a Monoclonal Antibody (Catalog # MAB6903). The ND₅₀ is typically 0.005-0.02 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_lightbox"> 690-PT) induces cytotoxicity in the the PK-15 porcine kidney epithelial cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Porcine TNF-a (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine TNF-a Monoclonal Antibody (Catalog # MAB6903). The ND₅₀ is typically 0.005-0.02 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." alt="Recombinant Porcine TNF-a (Catalog # 690-PT) induces cytotoxicity in the the PK-15 porcine kidney epithelial cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Porcine TNF-a (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine TNF-a Monoclonal Antibody (Catalog # MAB6903). The ND₅₀ is typically 0.005-0.02 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

690-PT) was serially diluted and captured by Mouse Anti-Porcine TNF‑ alpha Monoclonal Antibody (Catalog # MAB6902) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Porcine TNF‑ alpha Monoclonal Antibody (Catalog # MAB6903) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 690-PT) was serially diluted and captured by Mouse Anti-Porcine TNF‑ alpha Monoclonal Antibody (Catalog # MAB6902) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Porcine TNF‑ alpha Monoclonal Antibody (Catalog # MAB6903) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

1507-CT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Canine TNF-a (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine TNF-a Monoclonal Antibody (Catalog # MAB1507). The ND₅₀ is typically 1.25-5.0 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_lightbox"> 1507-CT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Canine TNF-a (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine TNF-a Monoclonal Antibody (Catalog # MAB1507). The ND₅₀ is typically 1.25-5.0 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." alt="Recombinant Canine TNF-a (Catalog # 1507-CT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Canine TNF-a (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine TNF-a Monoclonal Antibody (Catalog # MAB1507). The ND₅₀ is typically 1.25-5.0 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell secretion. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cell secretion. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

1507-CT) was serially diluted and captured by Mouse Anti-Canine TNF‑ alpha Monoclonal Antibody (Catalog # MAB1507) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Canine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1507) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1507-CT) was serially diluted and captured by Mouse Anti-Canine TNF‑ alpha Monoclonal Antibody (Catalog # MAB1507) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Canine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1507) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="TNF-a was detected in immersion fixed bovine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Bovine TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2279) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

2279-BT) was serially diluted and captured by Goat Anti-Bovine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2279) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Bovine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2279) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 2279-BT) was serially diluted and captured by Goat Anti-Bovine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2279) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Bovine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2279) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

2279-BT) was serially diluted and captured by Goat Anti-Bovine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2279) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Bovine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2279) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 2279-BT) was serially diluted and captured by Goat Anti-Bovine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2279) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Bovine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2279) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

690-PT) was serially diluted and captured by Mouse Anti-Porcine TNF‑ alpha Monoclonal Antibody (Catalog # MAB6902) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Porcine TNF‑ alpha Monoclonal Antibody (Catalog # MAB6903) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 690-PT) was serially diluted and captured by Mouse Anti-Porcine TNF‑ alpha Monoclonal Antibody (Catalog # MAB6902) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Porcine TNF‑ alpha Monoclonal Antibody (Catalog # MAB6903) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Porcine TNF‑ alpha (Catalog # 690-PT) was serially diluted and captured by Mouse Anti-Porcine TNF‑ alpha Monoclonal Antibody (Catalog # MAB6902) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Porcine TNF‑ alpha Monoclonal Antibody (Catalog # MAB6903) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />