Leptin R Antibodies

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="Leptin R was detected in immersion fixed frozen sections of mouse brain using Goat Anti-Mouse Leptin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF497) at 1 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cells in the choroid plexus. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cells in the choroid plexus. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

AF497) followed by Allophycocyanin-conjugated anti-Goat IgG Secondary Antibody (F0108). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_lightbox"> AF497) followed by Allophycocyanin-conjugated anti-Goat IgG Secondary Antibody (F0108). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_thumb" />

497-LR) was serially diluted and captured by Rat Anti-Mouse Leptin R Monoclonal Antibody (Catalog # MAB497) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Leptin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF497) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 497-LR) was serially diluted and captured by Rat Anti-Mouse Leptin R Monoclonal Antibody (Catalog # MAB497) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Leptin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF497) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

389-LR) and Recombinant Mouse Leptin R Fc Chimera (Catalog # 497-LR). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human Leptin R Monoclonal Antibody (Catalog # MAB867) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Leptin R at approximately 155-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox"> 389-LR) and Recombinant Mouse Leptin R Fc Chimera (Catalog # 497-LR). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human Leptin R Monoclonal Antibody (Catalog # MAB867) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Leptin R at approximately 155-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows 25 ng of Recombinant Human Leptin R Fc Chimera (Catalog # 389-LR) and Recombinant Mouse Leptin R Fc Chimera (Catalog # 497-LR). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human Leptin R Monoclonal Antibody (Catalog # MAB867) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Leptin R at approximately 155-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface of neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface of neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

MAB004) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> MAB004) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

389-LR), Recombinant Mouse Leptin R Fc Chimera (Catalog # 7814-LR) and Recombinant Rat Leptin R. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human Leptin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF389) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Leptin R at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox"> 389-LR), Recombinant Mouse Leptin R Fc Chimera (Catalog # 7814-LR) and Recombinant Rat Leptin R. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human Leptin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF389) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Leptin R at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows 25 ng of Recombinant Human Leptin R Fc Chimera (Catalog # 389-LR), Recombinant Mouse Leptin R Fc Chimera (Catalog # 7814-LR) and Recombinant Rat Leptin R. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human Leptin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF389) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Leptin R at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

MAB006), followed by Allophycocyanin-conjugated anti-Rat IgG Secondary Antibody (F0113). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_lightbox"> MAB006), followed by Allophycocyanin-conjugated anti-Rat IgG Secondary Antibody (F0113). Staining was performed using our Staining Membrane-associated Proteins protocol. " alt="HEK293 human cell line transfected with Mouse Leptin R and EGFP was stained with (A) Rat Anti-Mouse Leptin R Biotinylated Monoclonal Antibody (Catalog # MAB497) or (B) isotype control antibody (MAB006), followed by Allophycocyanin-conjugated anti-Rat IgG Secondary Antibody (F0113). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_thumb" />

497-LR) was serially diluted and captured by Rat Anti-Mouse Leptin R Monoclonal Antibody (Catalog # MAB497) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Leptin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF497) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 497-LR) was serially diluted and captured by Rat Anti-Mouse Leptin R Monoclonal Antibody (Catalog # MAB497) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Leptin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF497) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell bodies and processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell bodies and processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="Leptin R was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human Leptin R Monoclonal Antibody (Catalog # MAB867R) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell bodies and processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

398-LP) stimulates proliferation in the BaF3 mouse pro-B cell line transfected with human Leptin R in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR0002). Proliferation elicited by Recombinant Human Leptin/OB (10 ng/mL) is neutralized (green line) by increasing concentrations of Human Leptin R Monoclonal Antibody (Catalog # MAB86712). The ND₅₀ is typically 0.2-1 µg/mL." class="big_lightbox"> 398-LP) stimulates proliferation in the BaF3 mouse pro-B cell line transfected with human Leptin R in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR0002). Proliferation elicited by Recombinant Human Leptin/OB (10 ng/mL) is neutralized (green line) by increasing concentrations of Human Leptin R Monoclonal Antibody (Catalog # MAB86712). The ND₅₀ is typically 0.2-1 µg/mL." alt="Recombinant Human Leptin/OB (Catalog # 398-LP) stimulates proliferation in the BaF3 mouse pro-B cell line transfected with human Leptin R in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR0002). Proliferation elicited by Recombinant Human Leptin/OB (10 ng/mL) is neutralized (green line) by increasing concentrations of Human Leptin R Monoclonal Antibody (Catalog # MAB86712). The ND₅₀ is typically 0.2-1 µg/mL." class="big_thumb" />