FAB3832P) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> FAB3832P) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." alt="PBMC's + LPS (1ug/mL;24 hrs) and monensin (3 hrs) (A) vs untreated PBMC's (B) were stained with Mouse Anti-Human/Mouse IL‑12/IL‑35 p35 Monoclonal Antibody (Catalog # mab1570) and Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

CTS017) and counterstained with hemotoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS017) and counterstained with hemotoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="IL-12/IL-35 p35 was detected in immersion fixed frozen sections of mouse spleen using Rat Anti-Mouse IL-12/IL-35 p35 Monoclonal Antibody (Catalog # MAB6688) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hemotoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

285-IF, 75 ng/mL for 2 hours), then LPS (1 µg/mL for 12 hours) and lastly monensin (3 µM for 3 hours), or B) untreated, were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and Rabbit Anti-Human IL-12/IL-35 p35 Monoclonal Antibody (Catalog # MAB15701) followed by APC-conjugated Goat Anti-Rabbit Secondary Antibody (Catalog # F0111). Quadrant markers were set based on isotype control antibody (Catalog # MAB1050, data not shown). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> 285-IF, 75 ng/mL for 2 hours), then LPS (1 µg/mL for 12 hours) and lastly monensin (3 µM for 3 hours), or B) untreated, were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and Rabbit Anti-Human IL-12/IL-35 p35 Monoclonal Antibody (Catalog # MAB15701) followed by APC-conjugated Goat Anti-Rabbit Secondary Antibody (Catalog # F0111). Quadrant markers were set based on isotype control antibody (Catalog # MAB1050, data not shown). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." alt="Human peripheral blood mononuclear cells (PBMCs), A) treated with recombinant human IFN- gamma (Catalog # 285-IF, 75 ng/mL for 2 hours), then LPS (1 µg/mL for 12 hours) and lastly monensin (3 µM for 3 hours), or B) untreated, were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and Rabbit Anti-Human IL-12/IL-35 p35 Monoclonal Antibody (Catalog # MAB15701) followed by APC-conjugated Goat Anti-Rabbit Secondary Antibody (Catalog # F0111). Quadrant markers were set based on isotype control antibody (Catalog # MAB1050, data not shown). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />