IL-1 alpha/IL-1F1 Antibodies

400-ML), Recombinant Human IL-1a/IL-1F1 (200-LA) and Recombinant Rat IL-1a/IL-1F1 (500-RL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for IL-1a/IL-1F1 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox"> 400-ML), Recombinant Human IL-1a/IL-1F1 (200-LA) and Recombinant Rat IL-1a/IL-1F1 (500-RL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for IL-1a/IL-1F1 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows 50 ng of Recombinant Mouse IL-1a/IL-1F1 (400-ML), Recombinant Human IL-1a/IL-1F1 (200-LA) and Recombinant Rat IL-1a/IL-1F1 (500-RL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for IL-1a/IL-1F1 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA). The ND₅₀ is typically 0.002‑0.004 µg/mL." class="big_lightbox"> 400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA). The ND₅₀ is typically 0.002‑0.004 µg/mL." class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

400-ML) was serially diluted and captured by Rat Anti-Mouse IL‑1 alpha /IL‑1F1 Monoclonal Antibody (Catalog # MAB400) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 400-ML) was serially diluted and captured by Rat Anti-Mouse IL‑1 alpha /IL‑1F1 Monoclonal Antibody (Catalog # MAB400) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1a/IL-1F1 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-1a/IL-1F1 Monoclonal Antibody (Catalog # MAB200). The ND₅₀ is typically 0.05-0.15 µg/mL." class="big_lightbox"> 200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1a/IL-1F1 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-1a/IL-1F1 Monoclonal Antibody (Catalog # MAB200). The ND₅₀ is typically 0.05-0.15 µg/mL." alt="Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1a/IL-1F1 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-1a/IL-1F1 Monoclonal Antibody (Catalog # MAB200). The ND₅₀ is typically 0.05-0.15 µg/mL." class="big_thumb" />

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

200-LA) was serially diluted and captured by Mouse Anti-Human IL‑1 alpha /IL‑1F1 Monoclonal Antibody (Catalog # MAB200) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-200-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 200-LA) was serially diluted and captured by Mouse Anti-Human IL‑1 alpha /IL‑1F1 Monoclonal Antibody (Catalog # MAB200) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-200-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-200-NA). The ND₅₀ is typically 0.75-4.5 ng/mL." class="big_lightbox"> 200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-200-NA). The ND₅₀ is typically 0.75-4.5 ng/mL." alt="Recombinant Human IL-1a/IL-1F1 (200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-200-NA). The ND₅₀ is typically 0.75-4.5 ng/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

200-LA) was serially diluted and captured by Mouse Anti-Human IL‑1 alpha /IL‑1F1 Monoclonal Antibody (Catalog # MAB200) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-200-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 200-LA) was serially diluted and captured by Mouse Anti-Human IL‑1 alpha /IL‑1F1 Monoclonal Antibody (Catalog # MAB200) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-200-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-1a/IL-1F1 Monoclonal Antibody (Catalog # MAB400). The ND₅₀ is typically 0.2-0.6 µg/mL." class="big_lightbox"> 400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-1a/IL-1F1 Monoclonal Antibody (Catalog # MAB400). The ND₅₀ is typically 0.2-0.6 µg/mL." alt="Recombinant Mouse IL-1a/IL-1F1 (Catalog # 400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-1a/IL-1F1 Monoclonal Antibody (Catalog # MAB400). The ND₅₀ is typically 0.2-0.6 µg/mL." class="big_thumb" />

400-ML) was serially diluted and captured by Rat Anti-Mouse IL‑1 alpha /IL‑1F1 Monoclonal Antibody (Catalog # MAB400) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 400-ML) was serially diluted and captured by Rat Anti-Mouse IL‑1 alpha /IL‑1F1 Monoclonal Antibody (Catalog # MAB400) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse IL-1a/IL-1F1 Monoclonal Antibody (Catalog # MAB4001). The ND₅₀ is typically 0.005-0.02 µg/mL." class="big_lightbox"> 400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse IL-1a/IL-1F1 Monoclonal Antibody (Catalog # MAB4001). The ND₅₀ is typically 0.005-0.02 µg/mL." alt="Recombinant Mouse IL-1a/IL-1F1 (Catalog # 400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse IL-1a/IL-1F1 Monoclonal Antibody (Catalog # MAB4001). The ND₅₀ is typically 0.005-0.02 µg/mL." class="big_thumb" />

400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1a/IL-1F1 Polyclonal Antibody (Catalog # AB-400-NA). The ND₅₀ is typically 0.04-0.2 µg/mL." class="big_lightbox"> 400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1a/IL-1F1 Polyclonal Antibody (Catalog # AB-400-NA). The ND₅₀ is typically 0.04-0.2 µg/mL." alt="Recombinant Mouse IL-1a/IL-1F1 (Catalog # 400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1a/IL-1F1 Polyclonal Antibody (Catalog # AB-400-NA). The ND₅₀ is typically 0.04-0.2 µg/mL." class="big_thumb" />

200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Human IL-1a/IL-1F1 Polyclonal Antibody (Catalog # AB-200-NA). The ND₅₀ is typically 0.008-0.02 µg/mL." class="big_lightbox"> 200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Human IL-1a/IL-1F1 Polyclonal Antibody (Catalog # AB-200-NA). The ND₅₀ is typically 0.008-0.02 µg/mL." alt="Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Human IL-1a/IL-1F1 Polyclonal Antibody (Catalog # AB-200-NA). The ND₅₀ is typically 0.008-0.02 µg/mL." class="big_thumb" />

500-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF500). The ND₅₀ is typically 0.003-0.009 µg/mL." class="big_lightbox"> 500-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF500). The ND₅₀ is typically 0.003-0.009 µg/mL." alt="Recombinant Rat IL-1a/IL-1F1 (Catalog # 500-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF500). The ND₅₀ is typically 0.003-0.009 µg/mL." class="big_thumb" />

500-RL) was serially diluted and captured by Mouse Anti-Rat IL‑1 alpha /IL‑1F1 Monoclonal Antibody (Catalog # MAB500) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF500) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 500-RL) was serially diluted and captured by Mouse Anti-Rat IL‑1 alpha /IL‑1F1 Monoclonal Antibody (Catalog # MAB500) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF500) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

680-PI) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-1a/IL-1F1 (75 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF680). The ND₅₀ is typically 0.006-0.012 µg/mL." class="big_lightbox"> 680-PI) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-1a/IL-1F1 (75 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF680). The ND₅₀ is typically 0.006-0.012 µg/mL." alt="Recombinant Porcine IL-1a/IL-1F1 (Catalog # 680-PI) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-1a/IL-1F1 (75 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF680). The ND₅₀ is typically 0.006-0.012 µg/mL." class="big_thumb" />

5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010) To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox"> 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010) To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." alt="Human peripheral blood mononuclear cells were treated for 6 hours with 1 µg/mL LPS then stained with Human IL-1a/IL-1F1 Propeptide Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4154, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010) To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />

500-RL) was serially diluted and captured by Mouse Anti-Rat IL‑1 alpha /IL‑1F1 Monoclonal Antibody (Catalog # MAB500) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF500) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 500-RL) was serially diluted and captured by Mouse Anti-Rat IL‑1 alpha /IL‑1F1 Monoclonal Antibody (Catalog # MAB500) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF500) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Rat IL‑1 alpha /IL‑1F1 (Catalog # 500-RL) was serially diluted and captured by Mouse Anti-Rat IL‑1 alpha /IL‑1F1 Monoclonal Antibody (Catalog # MAB500) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF500) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />