IL-1 alpha/IL-1F1 Antibodies

200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Human IL-1a/IL-1F1 Membrane Form Monoclonal Antibody (Catalog # MAB2001). The ND₅₀ is typically 5-20 µg/mL." class="big_lightbox"> 200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Human IL-1a/IL-1F1 Membrane Form Monoclonal Antibody (Catalog # MAB2001). The ND₅₀ is typically 5-20 µg/mL." alt="Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Human IL-1a/IL-1F1 Membrane Form Monoclonal Antibody (Catalog # MAB2001). The ND₅₀ is typically 5-20 µg/mL." class="big_thumb" />

552-R1) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF552). The ND₅₀ is typically 0.006-0.036 µg/mL." class="big_lightbox"> 552-R1) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF552). The ND₅₀ is typically 0.006-0.036 µg/mL." alt="Recombinant Cotton Rat IL-1a/IL-1F1 (Catalog # 552-R1) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF552). The ND₅₀ is typically 0.006-0.036 µg/mL." class="big_thumb" />

552-R1) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Cotton Rat IL-1a/IL-1F1 Monoclonal Antibody (Catalog # MAB552). The ND₅₀ is typically 1-5 µg/mL." class="big_lightbox"> 552-R1) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Cotton Rat IL-1a/IL-1F1 Monoclonal Antibody (Catalog # MAB552). The ND₅₀ is typically 1-5 µg/mL." alt="Recombinant Cotton Rat IL-1a/IL-1F1 (Catalog # 552-R1) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Cotton Rat IL-1a/IL-1F1 Monoclonal Antibody (Catalog # MAB552). The ND₅₀ is typically 1-5 µg/mL." class="big_thumb" />

F0135). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol." class="big_lightbox"> F0135). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol." alt="Human peripheral blood mononuclear cells were (A) treated overnight with 1 µg/mL LPS or (B) untreated, then stained with Human IL-1a/IL-1F1 (Bermekimab) Monoclonal Antibody (Catalog # MAB10568, filled histogram) or unstained (open histogram), followed by Anti-Human IgG Allophycoerythrin-conjugated Secondary Antibody (F0135). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="IL‑1 alpha /IL‑1F1 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human IL‑1 alpha /IL‑1F1 Monoclonal Antibody (Catalog # MAB2002) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

HAF018). A specific band was detected for IL‑1 alpha /IL‑1F1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for IL‑1 alpha /IL‑1F1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of human skeletal muscle. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL‑1 alpha /IL‑1F1 Monoclonal Antibody (Catalog # MAB10891) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for IL‑1 alpha /IL‑1F1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL‑1 alpha /IL‑1F1 was detected in immersion fixed RT‑4 human urinary bladder transitional cell papilloma cell line (positive staining) and THP‑1 human acute monocytic leukemia cell line (negative staining) using Mouse Anti-Human IL‑1 alpha /IL‑1F1 Monoclonal Antibody (Catalog # MAB2003) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-1a/IL-1F1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-1a/IL-1F1 Recombinant Monoclonal Antibody (Catalog # MAB200R) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by the Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Human IL-1a/IL-1F1 Recombinant Monoclonal Antibody (Catalog # MAB200R). The ND₅₀ is typically 0.05-0.15 µg/mL." class="big_lightbox"> 200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by the Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Human IL-1a/IL-1F1 Recombinant Monoclonal Antibody (Catalog # MAB200R). The ND₅₀ is typically 0.05-0.15 µg/mL." class="big_thumb" />