| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24697SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26418-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26227-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21599-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26046 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-171-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-30141SS | |||
| Azide and BSA Free | NBP2-80769 | |||
![Western Blot: Histone H3.3 Antibody [NBP2-24697] - Total protein from human HeLa and A431 cells and mouse Neuro2A cells was separated on a 4-15% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-Histone H3.3 in blocking buffer with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Histone-H3.3-Antibody-Western-Blot-NBP2-24697-img0003.jpg "Western Blot: Histone H3.3 Antibody [NBP2-24697] - Total protein from human HeLa and A431 cells and mouse Neuro2A cells was separated on a 4-15% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-Histone H3.3 in blocking buffer with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody [NBP2-24697] - Human small intestine stained with Histone H3.3 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/Histone-H3.3-Antibody-Immunohistochemistry-Paraffin-NBP2-24697-img0002.jpg)
![Western Blot: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Western blot analysis of Histone H3.3 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Western-Blot-NBP2-67530-img0008.jpg "Western Blot: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Western blot analysis of Histone H3.3 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Staining Histone H3.3 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunocytochemistry-Immunofluorescence-NBP2-67530-img0006.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human placenta tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0014.jpg)
![Western Blot: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of Histone H3.3 on BT-20 cell lysates using anti-Histone H3.3 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Western-Blot-NBP2-67530-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Staining Histone H3.3 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunocytochemistry-Immunofluorescence-NBP2-67530-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0002.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded mouse brain tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded mouse colon tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded mouse stomach tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0005.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0009.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0010.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human lung tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0011.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human cervix tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0012.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human skin tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-ST50-08-Immunohistochemistry-Paraffin-NBP2-67530-img0013.jpg)
![Western Blot: Histone H3.3 Antibody (5C5B8) [NBP3-16489] - Western blot analysis of extracts of various cell lines, using Histone H3.3 Rabbit mAb (NBP3-16489) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-5C5B8-Western-Blot-NBP3-16489-img0005.jpg "Western Blot: Histone H3.3 Antibody (5C5B8) [NBP3-16489] - Western blot analysis of extracts of various cell lines, using Histone H3.3 Rabbit mAb (NBP3-16489) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: Histone H3.3 Antibody (5C5B8) [NBP3-16489] - Western blot analysis of extracts of Rat lung, using Histone H3.3 Rabbit mAb (NBP3-16489) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-5C5B8-Western-Blot-NBP3-16489-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (5C5B8) [NBP3-16489] - Immunohistochemistry of paraffin-embedded rat kidney using Histone H3.3 Rabbit mAb (NBP3-16489) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-5C5B8-Immunohistochemistry-Paraffin-NBP3-16489-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (5C5B8) [NBP3-16489] - Immunohistochemistry of paraffin-embedded human esophageal using Histone H3.3 Rabbit mAb (NBP3-16489) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-5C5B8-Immunohistochemistry-Paraffin-NBP3-16489-img0002.jpg)
![Chromatin Immunoprecipitation: Histone H3.3 Antibody (5C5B8) [Histone H3.3] - Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and Histone H3.3 Rabbit mAb . The ChIP sequencing results indicate the enrichment pattern of Histone H3.3 in selected genomic region and representative gene loci (GAPDH), as shown in figure.](http://images.novusbio.com/fullsize/nbp3-16489_rabbit-histone-h3-3-mab-5c5b8-5220251718458.jpg)
![Western Blot: Histone H3.3 [p Thr3] Antibody (28H4) [NBP3-26418] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, NIH/3T3 whole cell lysate. All lanes: Histone H3.3 [p Thr3] Antibody at 1.41 ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 16 KDa Observed band size: 16 KDa](http://images.novusbio.com/fullsize/nbp3-26418_rabbit-histone-h3-3-p-thr-3-mab-28h4-282202411472125.jpg "Western Blot: Histone H3.3 [p Thr3] Antibody (28H4) [NBP3-26418] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, NIH/3T3 whole cell lysate. All lanes: Histone H3.3 [p Thr3] Antibody at 1.41 ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 16 KDa Observed band size: 16 KDa")
![Immunocytochemistry/Immunofluorescence: Histone H3.3 [p Thr3] Antibody (28H4) [NBP3-26418] - Analysis of Histone H3.3 [p Thr3] Antibody (28H4) diluted at 1:100 and staining in Hela cells. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26418_rabbit-histone-h3-3-p-thr-3-mab-28h4-28220241323624.jpg)
![Flow Cytometry: Histone H3.3 [p Thr3] Antibody (28H4) [NBP3-26418] - Overlay histogram showing Hela cells stained with Histone H3.3 [p Thr3] Antibody (28H4) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26418_rabbit-histone-h3-3-p-thr-3-mab-28h4-282202414183663.jpg)
![Immunohistochemistry: Histone H3.3 Antibody (4H2) [NBP3-26227] - Image of Histone H3.3 Antibody (4H2) diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26227_rabbit-histone-h3-3-mab-4h2-262202415475637.jpg "Immunohistochemistry: Histone H3.3 Antibody (4H2) [NBP3-26227] - Image of Histone H3.3 Antibody (4H2) diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Immunohistochemistry: Histone H3.3 Antibody (4H2) [NBP3-26227] - Image of Histone H3.3 Antibody (4H2) diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26227_rabbit-histone-h3-3-mab-4h2-27220248203911.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3.3 Antibody (4H2) [NBP3-26227] - Staining of Hela cells with Histone H3.3 Antibody (4H2) at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26227_rabbit-histone-h3-3-mab-4h2-282202412493619.jpg)
![Western Blot: Histone H3.3 [p Ser 31] Antibody (SR1217) [NBP3-21599] - Western blot analysis using NBP3-21599 in HeLa cell treated with Calyculin A lysate.](http://images.novusbio.com/fullsize/nbp3-21599_rabbit-histone-h3-3-p-ser-31-mab-sr1217-57202317215133.jpg "Western Blot: Histone H3.3 [p Ser 31] Antibody (SR1217) [NBP3-21599] - Western blot analysis using NBP3-21599 in HeLa cell treated with Calyculin A lysate.")
![Western Blot: Histone H3.3 Antibody (S04-5J3) [NBP3-14894] - Western blot detection of Histone H3. 3 in K562, C6, 3T3 cell lysates using Histone H3. 3 Rabbit mAb (1:1000 diluted). Predicted band size: 15kDa. Observed band size: 15kDa.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-S04-5J3-Western-Blot-NBP3-14894-img0001.jpg "Western Blot: Histone H3.3 Antibody (S04-5J3) [NBP3-14894] - Western blot detection of Histone H3. 3 in K562, C6, 3T3 cell lysates using Histone H3. 3 Rabbit mAb (1:1000 diluted). Predicted band size: 15kDa. Observed band size: 15kDa.")
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody (S04-5J3) [NBP3-14894] - Immunohistochemistry of Histone H3. 3 in paraffin-embedded Human lung cancer tissue using Histone H3. 3 Rabbit mAb at dilution 1/50](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-S04-5J3-Immunohistochemistry-Paraffin-NBP3-14894-img0002.jpg)
![Western Blot: Histone H3.3 Antibody (RM190) - Azide and BSA Free [NBP3-26046] - Western Blot of HeLa whole cell lysate, recombinant Histone H3.3, and Histone H3.1 proteins, using RM190 at 1 ug/mL, showed a band of histone H3.3 in HeLa cells and recombinant H3.3 protein, and no cross activity with Histone H3.1.](http://images.novusbio.com/fullsize/nbp3-26046_rabbit-histone-h3-3-mab-rm190-azide-and-bsa-free-2262024231103.png "Western Blot: Histone H3.3 Antibody (RM190) - Azide and BSA Free [NBP3-26046] - Western Blot of HeLa whole cell lysate, recombinant Histone H3.3, and Histone H3.1 proteins, using RM190 at 1 ug/mL, showed a band of histone H3.3 in HeLa cells and recombinant H3.3 protein, and no cross activity with Histone H3.1.")
![Multiplex Immunoassay: Histone H3.3 Antibody (RM190) - Azide and BSA Free [NBP3-26046] - RM190 reacts specifically to Histone H3.3. No cross reactivity with Histone H3.1.](http://images.novusbio.com/fullsize/nbp3-26046_rabbit-histone-h3-3-mab-rm190-azide-and-bsa-free-24620241245913.png)
![Western Blot: Histone H3.3 Antibody (190) [NBP2-61548] - HeLa whole cell lysate, recombinant Histone H3.3 and Histone H3.1 proteins, using 190 at 1 ug/mL, showed a band of histone H3.3 in HeLa cells and recombinant H3.3 protein, and no cross activity with Histone H3.1.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-190-Western-Blot-NBP2-61548-img0003.jpg "Western Blot: Histone H3.3 Antibody (190) [NBP2-61548] - HeLa whole cell lysate, recombinant Histone H3.3 and Histone H3.1 proteins, using 190 at 1 ug/mL, showed a band of histone H3.3 in HeLa cells and recombinant H3.3 protein, and no cross activity with Histone H3.1.")
![ELISA: Histone H3.3 Antibody (190) [NBP2-61548] - Reacts specifically to Histone H3.3. No cross reactivity with Histone H3.1.](http://images.novusbio.com/fullsize/Histone-H3-3-Antibody-190-ELISA-NBP2-61548-img0001.jpg)
![Western Blot: Histone H3 Antibody [NB500-171] - Analysis of HeLa, COS, C6, and K562 cell lysate using histone H3 antibody [NB500-171] antibody at 1:100. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-171-img0013.jpg "Western Blot: Histone H3 Antibody [NB500-171] - Analysis of HeLa, COS, C6, and K562 cell lysate using histone H3 antibody [NB500-171] antibody at 1:100. Observed molecular weight is ~17 kDa.")
![Chromatin Immunoprecipitation: Histone H3 Antibody [NB500-171] - Chromatin from one million formaldehyde cross-linked HeLa cells was precipitated using 2 ug of NB500-171 and 25 uL of magnetic IgG beads, using standard ChIP methods. A similar sample containing no antibody (No Ab) was included as a negative control. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin. Representative target genes from active, inactive, and heterochromatic regions of the genome show amplification, indicative of the presence of Histone H3.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Chromatin-Immunoprecipitation-NB500-171-img0016.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE mouse testis.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-Paraffin-NB500-171-img0017.jpg)
![Western Blot: Histone H3 Antibody [NB500-171] - MDA-MB-231 cells were treated with vehicle (V) or paclitaxel (P). Cytosolic and nuclear lysates were prepared, and immunoblot assay was performed. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-171-img0020.jpg)
![Immunohistochemistry: Histone H3 Antibody [NB500-171] - Analysis of Histone H3 in human breast cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-NB500-171-img0012.jpg)
![Western Blot: Histone H3 Antibody [NB500-171] - Reduced histone levels in senescent cells is induced in vitro by different means, and in vivo from aged donors. (b) Western immunoblot analyses of histones in fibroblasts described in (a). (c) Histone levels in dermal fibroblasts isolated from human neonatal (age 0, donors a and b) and adult donors. Anti- used at 1:20,000 and Anti-Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] (NBP2-16848) used at 1:10,000. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1038/s41598-020-59163-4) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-171-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody [NB500-171] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB500-171 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB500-171-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody [NB500-171] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB500-171 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB500-171-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody [NB500-171] - Immunofluorescence staining to detect histone H3 in human blood. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB500-171-img0023.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Analysis of Histone H3 antibody on Mouse pancreas tissue. Histone H3 antibody dilution 1:200. Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-Paraffin-NB500-171-img0025.jpg)
![Western Blot: Histone H3 Antibody [NB500-171] - (1) HeLa, (2) S. cerevisiae whole cell lysates, (3) Histones purified from HeLa cells. Theoretical molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-171-img0014.jpg)
![Western Blot: Histone H3 Antibody [NB500-171] - Total histone H3 levels in MCF-7 and HCT-116 cells. Observed molecular weight is ~17 kDa. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-171-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody [NB500-171] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-Histone H3 at 5 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB500-171-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody [NB500-171] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-Histone H3 at 5 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB500-171-img0019.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE rat pancreas.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-Paraffin-NB500-171-img0009.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE rat colon.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-Paraffin-NB500-171-img0010.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE mouse brain.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-Paraffin-NB500-171-img0007.jpg)
![Western Blot: Histone H3 Antibody - BSA Free [NB500-171] - p53 but not ATM is required for chronic gamma -radiation-induced histone reductions. (a) Western blots of histone proteins in wild-type, TP53−/− & ATM−/− RPE-1 cells following exposures to 20 mGy/h chronic radiation for 7 days. (b) ATM activation, as shown by auto-phosphorylation on S1981, in response to chronic radiation in primary fibroblasts. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32042076), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-171_rabbit-polyclonal-histone-h3-antibody-31020241539715.jpg)
![Western Blot: Histone H3 Antibody - BSA Free [NB500-171] - Reduced histone levels in senescent cells is induced in vitro by different means, & in vivo from aged donors. (a) Phase-contrast images of primary fibroblasts induced into senescence by chronic gamma -radiation, oncogene over-expression or exhaustive replication (replicative senescence), & DNA damage from a single acute 4 Gy X-ray dose at an early time-point (1 hour) as a control for DNA damage without senescence. Scale bars 200 µm. (b) Western immunoblot analyses of histones in fibroblasts described in (a). (c) Histone levels in dermal fibroblasts isolated from human neonatal (age 0, donors a & b) & adult donors. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32042076), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-171_rabbit-polyclonal-histone-h3-antibody-31020241534368.jpg)
![Western Blot: Histone H3 Antibody - BSA Free [NB500-171] - Reduced histone levels in senescent cells is induced in vitro by different means, & in vivo from aged donors. (a) Phase-contrast images of primary fibroblasts induced into senescence by chronic gamma -radiation, oncogene over-expression or exhaustive replication (replicative senescence), & DNA damage from a single acute 4 Gy X-ray dose at an early time-point (1 hour) as a control for DNA damage without senescence. Scale bars 200 µm. (b) Western immunoblot analyses of histones in fibroblasts described in (a). (c) Histone levels in dermal fibroblasts isolated from human neonatal (age 0, donors a & b) & adult donors. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32042076), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-171_rabbit-polyclonal-histone-h3-antibody-310202415291940.jpg)
![Western Blot: Histone H3 Antibody - BSA Free [NB500-171] - Chronic gamma -radiation reduces histone levels. (a) Western blot analyses of histone H2AX & gamma H2AX in primary fibroblasts exposed to various dose-rates of chronic gamma -radiation for 7 days. Cells irradiated with a single acute dose of 4 Gy X-ray were included as control. (b) Effect of chronic gamma -irradiation on H2AX levels in three different isogenic primary cell types from a different donor to that used in (a) at the same dose rates as in (a). (c) Immunoblots of other histones in chronically irradiated primary fibroblasts. (d) All significant histone level changes detected by SILAC LC-MS/MS protein analyses of samples from primary fibroblasts exposed or mock-exposed to chronic gamma -radiation for 7 days. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32042076), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-171_rabbit-polyclonal-histone-h3-antibody-310202415392548.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Western-Blot-NBP1-30141-img0012.jpg "Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Observed molecular weight is ~15 kDa.")
![Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Histone 3 was detected in immersion fixed paraffin-embedded sections of human colon cancer using anti-human mouse monoclonal antibody (Catalog # NBP1-30141) at 1:1000 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Immunohistochemistry-Paraffin-NBP1-30141-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-Histone H3 [Trymethyl Lys9] (6F12-H4) at 2 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (green) at a 1:500 dilution. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Immunocytochemistry-Immunofluorescence-NBP1-30141-img0011.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Analysis of Histone H3 (NBP1-30141) using HeLa nuclear lysate [NB800-PC9]. Observed molecular weight ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Western-Blot-NBP1-30141-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Analysis of A549 cells (fixed with methanol) using Histone H3 [Trimethyl Lys9] antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Immunocytochemistry-Immunofluorescence-NBP1-30141-img0014.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Used to perform ChIP on HeLa cells.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Chromatin-Immunoprecipitation-NBP1-30141-img0002.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - H3K9Me3 analysis of MCF7 and HCT116. Theorectical molecular weight is ~15 kDa. Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Western-Blot-NBP1-30141-img0008.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Representative Western blot showing M-track protein protein proximity signals obtained for Kic1. Hog1-protA-H3: background control, Nup2: positive control. Below: Proximity signals. n = 3 replicates per sample except when indicated differently. Ratios are log2-transformed. Black lines indicate average proximity signal. Proximity signals that differ significantly from background are marked in green (q 0.01) filled circles. Grey filled triangles: q > 0.05. N: N-terminal HKMTmyc fusion. Image collected and cropped by CiteAb from the following publication (//biosignaling.biomedcentral.com/articles/10.1186/s12964-019-0381-z), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Western-Blot-NBP1-30141-img0013.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Analysis of Histone H3 K9-me3 in HeLa histone lysates. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Western-Blot-NBP1-30141-img0004.jpg)
![Histone H3 [Trimethyl Lys9] (6F12-H4) was detected in immersion fixed MCF7 human breast cancer cell line using Mouse anti- Histone H3 [Trimethyl Lys9] (6F12-H4) Protein G Purified Monoclonal Antibody conjugated to DyLight 550 (Catalog # NBP1-30141R) (red) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/antibody/nbp1-30141_mouse-monoclonal-histone-h3-trimethyl-lys9-antibody-6f12-h4-immunocytochemistry-immunofluorescence-2382024134224..jpg)
![Western Blot: Histone H3.3B Antibody (2D7-H1) [H00003021-M01] - Analysis of H3F3B expression in transfected 293T cell line by H3F3B monoclonal antibody (M01), clone 2D7-H1.Lane 1: H3F3B transfected lysate(15 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Histone-H3-3B-Antibody-2D7-H1-Western-Blot-H00003021-M01-img0009.jpg "Western Blot: Histone H3.3B Antibody (2D7-H1) [H00003021-M01] - Analysis of H3F3B expression in transfected 293T cell line by H3F3B monoclonal antibody (M01), clone 2D7-H1.Lane 1: H3F3B transfected lysate(15 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Histone H3.3B Antibody (2D7-H1) [H00003021-M01] - Analysis of monoclonal antibody to H3F3B on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Histone-H3-3B-Antibody-2D7-H1-Immunocytochemistry-Immunofluorescence-H00003021-M01-img0005.jpg)
![Immunohistochemistry-Paraffin: Histone H3.3B Antibody (2D7-H1) [H00003021-M01] - Analysis of monoclonal antibody to H3F3B on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Histone-H3-3B-Antibody-2D7-H1-Immunohistochemistry-Paraffin-H00003021-M01-img0007.jpg)
![ELISA: Histone H3.3B Antibody (2D7-H1) [H00003021-M01] - Detection limit for recombinant GST tagged H3F3B is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Histone-H3-3B-Antibody-2D7-H1-ELISA-H00003021-M01-img0010.jpg)